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The map label for this gene is priA [H]

Identifier: 15603002

GI number: 15603002

Start: 1336037

End: 1338250

Strand: Reverse

Name: priA [H]

Synonym: PM1137

Alternate gene names: 15603002

Gene position: 1338250-1336037 (Counterclockwise)

Preceding gene: 15603004

Following gene: 15603001

Centisome position: 59.28

GC content: 42.64

Gene sequence:

>2214_bases
ATGCAACTCATCCGTGTGGCACTGGCAGTGCCGTTATATCGTTTTTTTGATTATCTTTTACCCCCTGAGCTTGATGCCGT
GATTGGGGGACGTGTCGTTGTGCCTTTTGGTAGGCAACAGCGTGTTGGGATTGTGGTGGCATTGCCTGTGGAAACAGAGG
TTGAAACGGCGCAATTAAAGTCAGTAATTGAGGTGTTGGATACTCAAAGCGTGTTTAATCAAGATATGTGGCAGTTATTG
CATTGGGCTGCTGGCTATTATCAAACTGCATTGGGTGAAGTGTTATTTCAAGCGTTGCCTGTCAAACTTAGGCAAGGGGA
AAGTGCGGTTGAAAAAGCGAAAATTTTTTGGCAATTGACCGAACGTGGTCTGCAAGCATTGGCACAACACGCTGACAGCG
GGGAGTTTAAGCGGGCAAAAAAACAGCTTGAGGTTCTCATCGTGTTATCTCAAACCCCACTAGAAAAAGGCAATAATCCG
TTTAATAGCGCGATTTTCTCAGCTCTTAAACAAAAAGATTACATAGAAGACGTTGAGGTTCAACCGCAGTTACACGCATG
GCAGACCGATTTCACCGATAAACCGATGGTGAATGACGAAAATCGATTACGGTTAAATAAGCAACAAGCATTAGCTTTCA
GCCAATTAGTGTTTCATCAAGGATTTGCGGTGTGGTTACTCGATGGTGTAACGGGATCAGGAAAAACCGAGATTTATTTG
CAACTGATTGAAGAGGTGTTGAAAAAAGGCAAACAAATATTAGTGCTTGTGCCTGAAATTGGCTTAACACCACAAACAGT
ACAACGTTTTGTCGCTCGTTTTCAGGTGGTGATTGATGTATTGCATTCTAATCTAAATGATACCCAACGTTTGGCAGTAT
GGCAGCGTGCAAGAACTGGACAAAGTGCGATTGTCATCGGCACAAGATCCGCATTATTTACGCAATTTGCTGATTTAGGA
TTGATTGTTATTGATGAAGAGCATGATGCCTCTTTCAAACAACAAGACAATTTCCGTTATCATGCTCGTGACTTGTCGGT
TGTATTGGCGAAACAGTTAAATATTCCAGTGTTAATGGGGTCAGCGACACCGAGTTTAGAAAGTCTAAATAATGTTCATA
ATGGCAAGTATCGCCATATTATTTTATCAAACAGAGCGGGTAATGCTGCATTACTACGTCATTCTGTGATCGACTTAAAG
CAACAAGTTGTGCGTAATGGCTTATCTGAAGCGTTGTTACAGCGAATGGAAAAGCACCTACAAAAAGGTAACCAAGTGTT
ACTTTTTTTAAATCGGCGTGGTTTTGCTCCCGTGATGTTATGCCATGAATGTGGGTGGATTGCCGAGTGCCAACATTGTG
AGAAACCTTATACTTATCATCAACATCAGCGTGCTTTACGCTGTCATCACTGTGCGGCACAAAAACCGATTCCGCGGCAA
TGTGAGCATTGTGGGTCCACACATTTGATCACCACTGGGTTGGGAACTGAGCAATTAGAGCAAACTTTAGCAAGTCGTTT
TCCACAATATCAAATTACACGTATTGATCGTGACAGTACGGCACGAAAAGGCAAATTAGAGGATTATCTCACCGATATTC
AACAGGGGAAAAGTCAAATTCTGATTGGTACACAAATGCTCGCAAAAGGACACCATTTTCCTAATGTGACGTTAGTGGCG
TTAGTGAACGTAGATAGTGCCTTATTCTCTTTAGATTTTCGTGCAGAAGAGCGCTTGGCACAACTTTATGTACAAGTGGC
AGGTCGTGCAGGTCGTGTGGAGAAACAAGGGGAAGTGGTGCTACAAACCCATTACCCAGATCATCCATTATTACAGCGTT
TGTTGCAGCAAGGTTATGCAGATTTTGCGGAGTACGCGTTACAATTACGACAAAAAATGGGCTTGCCACCGTTTAGTGCA
CAAGCATTGTTTAAAGCACAAAGCAGGCATTCAGAAGACGCCGAGCAAGTCTTGCAGCAAGTTGCTGAGTATTTTTACAC
TTGGAATCAAAGCTCGCAGTGTGCGATACAAATTTTAGGACCGATTGCGGCACCTTTCAGTAAAAAAGCTGGTCAATATC
GTTGGCAATTATTGCTACAACATCCTTCCAGAGCCCAGTTACACCATGCCTTATCGGCATTTCAAGCAACATCAACGATT
AAATTCGGTTCAGTACGTTGTGTATTAGATGTGGATCCTATTGATTTAAATTAG

Upstream 100 bases:

>100_bases
TATGAGAAGAATTACTCTATGGCATATAATATAACAAAAATTATTCAATTTGAATGAAATGCATACAGGTTCTGTGTGAA
TTATTGGCAGAGTAAAAAAC

Downstream 100 bases:

>100_bases
CTTGTGTTTGAGTTATAAGGGATTTTTCAGTAGAATTGTGCCAATTTTTATGAATGTTTTTAGGAATAGGTTTTAGCGTG
GCAAGACGAAATTATGCCGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MQLIRVALAVPLYRFFDYLLPPELDAVIGGRVVVPFGRQQRVGIVVALPVETEVETAQLKSVIEVLDTQSVFNQDMWQLL
HWAAGYYQTALGEVLFQALPVKLRQGESAVEKAKIFWQLTERGLQALAQHADSGEFKRAKKQLEVLIVLSQTPLEKGNNP
FNSAIFSALKQKDYIEDVEVQPQLHAWQTDFTDKPMVNDENRLRLNKQQALAFSQLVFHQGFAVWLLDGVTGSGKTEIYL
QLIEEVLKKGKQILVLVPEIGLTPQTVQRFVARFQVVIDVLHSNLNDTQRLAVWQRARTGQSAIVIGTRSALFTQFADLG
LIVIDEEHDASFKQQDNFRYHARDLSVVLAKQLNIPVLMGSATPSLESLNNVHNGKYRHIILSNRAGNAALLRHSVIDLK
QQVVRNGLSEALLQRMEKHLQKGNQVLLFLNRRGFAPVMLCHECGWIAECQHCEKPYTYHQHQRALRCHHCAAQKPIPRQ
CEHCGSTHLITTGLGTEQLEQTLASRFPQYQITRIDRDSTARKGKLEDYLTDIQQGKSQILIGTQMLAKGHHFPNVTLVA
LVNVDSALFSLDFRAEERLAQLYVQVAGRAGRVEKQGEVVLQTHYPDHPLLQRLLQQGYADFAEYALQLRQKMGLPPFSA
QALFKAQSRHSEDAEQVLQQVAEYFYTWNQSSQCAIQILGPIAAPFSKKAGQYRWQLLLQHPSRAQLHHALSAFQATSTI
KFGSVRCVLDVDPIDLN

Sequences:

>Translated_737_residues
MQLIRVALAVPLYRFFDYLLPPELDAVIGGRVVVPFGRQQRVGIVVALPVETEVETAQLKSVIEVLDTQSVFNQDMWQLL
HWAAGYYQTALGEVLFQALPVKLRQGESAVEKAKIFWQLTERGLQALAQHADSGEFKRAKKQLEVLIVLSQTPLEKGNNP
FNSAIFSALKQKDYIEDVEVQPQLHAWQTDFTDKPMVNDENRLRLNKQQALAFSQLVFHQGFAVWLLDGVTGSGKTEIYL
QLIEEVLKKGKQILVLVPEIGLTPQTVQRFVARFQVVIDVLHSNLNDTQRLAVWQRARTGQSAIVIGTRSALFTQFADLG
LIVIDEEHDASFKQQDNFRYHARDLSVVLAKQLNIPVLMGSATPSLESLNNVHNGKYRHIILSNRAGNAALLRHSVIDLK
QQVVRNGLSEALLQRMEKHLQKGNQVLLFLNRRGFAPVMLCHECGWIAECQHCEKPYTYHQHQRALRCHHCAAQKPIPRQ
CEHCGSTHLITTGLGTEQLEQTLASRFPQYQITRIDRDSTARKGKLEDYLTDIQQGKSQILIGTQMLAKGHHFPNVTLVA
LVNVDSALFSLDFRAEERLAQLYVQVAGRAGRVEKQGEVVLQTHYPDHPLLQRLLQQGYADFAEYALQLRQKMGLPPFSA
QALFKAQSRHSEDAEQVLQQVAEYFYTWNQSSQCAIQILGPIAAPFSKKAGQYRWQLLLQHPSRAQLHHALSAFQATSTI
KFGSVRCVLDVDPIDLN
>Mature_737_residues
MQLIRVALAVPLYRFFDYLLPPELDAVIGGRVVVPFGRQQRVGIVVALPVETEVETAQLKSVIEVLDTQSVFNQDMWQLL
HWAAGYYQTALGEVLFQALPVKLRQGESAVEKAKIFWQLTERGLQALAQHADSGEFKRAKKQLEVLIVLSQTPLEKGNNP
FNSAIFSALKQKDYIEDVEVQPQLHAWQTDFTDKPMVNDENRLRLNKQQALAFSQLVFHQGFAVWLLDGVTGSGKTEIYL
QLIEEVLKKGKQILVLVPEIGLTPQTVQRFVARFQVVIDVLHSNLNDTQRLAVWQRARTGQSAIVIGTRSALFTQFADLG
LIVIDEEHDASFKQQDNFRYHARDLSVVLAKQLNIPVLMGSATPSLESLNNVHNGKYRHIILSNRAGNAALLRHSVIDLK
QQVVRNGLSEALLQRMEKHLQKGNQVLLFLNRRGFAPVMLCHECGWIAECQHCEKPYTYHQHQRALRCHHCAAQKPIPRQ
CEHCGSTHLITTGLGTEQLEQTLASRFPQYQITRIDRDSTARKGKLEDYLTDIQQGKSQILIGTQMLAKGHHFPNVTLVA
LVNVDSALFSLDFRAEERLAQLYVQVAGRAGRVEKQGEVVLQTHYPDHPLLQRLLQQGYADFAEYALQLRQKMGLPPFSA
QALFKAQSRHSEDAEQVLQQVAEYFYTWNQSSQCAIQILGPIAAPFSKKAGQYRWQLLLQHPSRAQLHHALSAFQATSTI
KFGSVRCVLDVDPIDLN

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=742, Percent_Identity=53.099730458221, Blast_Score=767, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83313; Mature: 83313

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIRVALAVPLYRFFDYLLPPELDAVIGGRVVVPFGRQQRVGIVVALPVETEVETAQLK
CHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCCCCEEEEEECCCCCHHHHHHH
SVIEVLDTQSVFNQDMWQLLHWAAGYYQTALGEVLFQALPVKLRQGESAVEKAKIFWQLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHH
ERGLQALAQHADSGEFKRAKKQLEVLIVLSQTPLEKGNNPFNSAIFSALKQKDYIEDVEV
HHHHHHHHHCCCCCHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
QPQLHAWQTDFTDKPMVNDENRLRLNKQQALAFSQLVFHQGFAVWLLDGVTGSGKTEIYL
CCCHHHCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
QLIEEVLKKGKQILVLVPEIGLTPQTVQRFVARFQVVIDVLHSNLNDTQRLAVWQRARTG
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
QSAIVIGTRSALFTQFADLGLIVIDEEHDASFKQQDNFRYHARDLSVVLAKQLNIPVLMG
CCEEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEC
SATPSLESLNNVHNGKYRHIILSNRAGNAALLRHSVIDLKQQVVRNGLSEALLQRMEKHL
CCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QKGNQVLLFLNRRGFAPVMLCHECGWIAECQHCEKPYTYHQHQRALRCHHCAAQKPIPRQ
CCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
CEHCGSTHLITTGLGTEQLEQTLASRFPQYQITRIDRDSTARKGKLEDYLTDIQQGKSQI
HHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEE
LIGTQMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYVQVAGRAGRVEKQGEVV
EEHHHHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEE
LQTHYPDHPLLQRLLQQGYADFAEYALQLRQKMGLPPFSAQALFKAQSRHSEDAEQVLQQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VAEYFYTWNQSSQCAIQILGPIAAPFSKKAGQYRWQLLLQHPSRAQLHHALSAFQATSTI
HHHHHHHCCCCCCEEEEEHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCEE
KFGSVRCVLDVDPIDLN
EECCEEEEEECCCCCCC
>Mature Secondary Structure
MQLIRVALAVPLYRFFDYLLPPELDAVIGGRVVVPFGRQQRVGIVVALPVETEVETAQLK
CHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCCCCEEEEEECCCCCHHHHHHH
SVIEVLDTQSVFNQDMWQLLHWAAGYYQTALGEVLFQALPVKLRQGESAVEKAKIFWQLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHH
ERGLQALAQHADSGEFKRAKKQLEVLIVLSQTPLEKGNNPFNSAIFSALKQKDYIEDVEV
HHHHHHHHHCCCCCHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
QPQLHAWQTDFTDKPMVNDENRLRLNKQQALAFSQLVFHQGFAVWLLDGVTGSGKTEIYL
CCCHHHCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
QLIEEVLKKGKQILVLVPEIGLTPQTVQRFVARFQVVIDVLHSNLNDTQRLAVWQRARTG
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
QSAIVIGTRSALFTQFADLGLIVIDEEHDASFKQQDNFRYHARDLSVVLAKQLNIPVLMG
CCEEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEC
SATPSLESLNNVHNGKYRHIILSNRAGNAALLRHSVIDLKQQVVRNGLSEALLQRMEKHL
CCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QKGNQVLLFLNRRGFAPVMLCHECGWIAECQHCEKPYTYHQHQRALRCHHCAAQKPIPRQ
CCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
CEHCGSTHLITTGLGTEQLEQTLASRFPQYQITRIDRDSTARKGKLEDYLTDIQQGKSQI
HHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEE
LIGTQMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYVQVAGRAGRVEKQGEVV
EEHHHHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEE
LQTHYPDHPLLQRLLQQGYADFAEYALQLRQKMGLPPFSAQALFKAQSRHSEDAEQVLQQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VAEYFYTWNQSSQCAIQILGPIAAPFSKKAGQYRWQLLLQHPSRAQLHHALSAFQATSTI
HHHHHHHCCCCCCEEEEEHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCEE
KFGSVRCVLDVDPIDLN
EECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]