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The map label for this gene is metB [H]

Identifier: 15602860

GI number: 15602860

Start: 1168033

End: 1169142

Strand: Reverse

Name: metB [H]

Synonym: PM0995

Alternate gene names: 15602860

Gene position: 1169142-1168033 (Counterclockwise)

Preceding gene: 15602861

Following gene: 15602859

Centisome position: 51.79

GC content: 40.72

Gene sequence:

>1110_bases
ATGACACAACATTATTCAATTGAAACGTTATTAGCGCAGGCTGGAAATCGAACGGATGAGCGTACTGGTGCAGTTTCTAC
ACCGATCTTTTTATCAACAGCTTATGCCCACCATGGAATTGGTGAAAGTACAGGCTATGACTATACACGTACGAAAAATC
CAACACGTAGCGTTTTAGAAGAAACCATTGCTAAATTAGAAGGTGGGGAACGAGGCTTTGCTTGTGCTTCAGGAATGGCG
GCAATTCAATTAATCATGTCCTTATTTACGTCCCCTGATGAGTGGATTGTTTCGCGTGATGTGTATGGTGGCACCTATCG
TTTATTGGATTTCGCGTACAAAAATACACAAGGCGTTAAACCAGTTTATGTGAATACATCAGAGGTATCCTGTATCGAAG
CGGCGATTACGTCTAATACAAAAGCAATTTTTGTAGAAACTCCTTCTAATCCTTTGATGGAGGAATGTGATGTAGCTGCG
ATTGCAAAAATTGCGAAAAAACATAATCTGCTTTTGATTGTCGATAATACTTTCTTAACACCGGTTTTATTTAGACCAAT
TGAGGCTGGTGCAGATATTGTGATTCATAGTGGAACTAAATACTTGGCTGGTCATAACGATGCTTTGGTTGGACTGGTTG
TGGCAAAAGGTGAAGAACTCTGCCAACGTTTATTCTATATTCAGAATGGGGCAGGTGCAGTTCTGTCGCCTTTCGATTCA
TGGTTAACGATTCGAGGCATGAAAACCCTCGCCTTACGTATGGAACGTCATGAACAAAATGCCAAACAGCTGGCTGCGTT
TCTGGCTTCTCAGCCACAAGTAAAAAATGTGTTATATCCAAATAAGGGCGGTATGCTTTCTTTCCGCTTACATGAAGCAC
ATTGGGTTAATCCTTTTTTGAAAGCGCTGAAACTAATCACTTTTGCTGAAAGTTTGGGGGGAACAGAGAGCTTTATTACT
TATCCAGCGACACAAACCCATATGGATATTCCAGAAGAGGAGCGTATTGCGCGCGGTGTGTGTAATTGTCTTTTGCGTTT
TTCTGTTGGTTTAGAAAATGTAGAGGATATTAAAGCGGATTTATTACAAGCGTTTGCACAATTAAACTAA

Upstream 100 bases:

>100_bases
TATTTTCTTTTTTCACTTTTATGACATAATATCAAGTCTAGACATCTAGATAGCTAAAATAATAAGTTTTTATTCTTTAC
TTAGGGAATACAGAGGTTCT

Downstream 100 bases:

>100_bases
GTTTTCCTCGTGGACGGATTGATTTGCATAATGCTTATGATATTATCATTGCACTTGAAATGATAAAAAATATTCTTATT
ATAAAGAATGTTATTAACAA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLEETIAKLEGGERGFACASGMA
AIQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVKPVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAA
IAKIAKKHNLLLIVDNTFLTPVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDS
WLTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYPNKGGMLSFRLHEAHWVNPFLKALKLITFAESLGGTESFIT
YPATQTHMDIPEEERIARGVCNCLLRFSVGLENVEDIKADLLQAFAQLN

Sequences:

>Translated_369_residues
MTQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLEETIAKLEGGERGFACASGMA
AIQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVKPVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAA
IAKIAKKHNLLLIVDNTFLTPVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDS
WLTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYPNKGGMLSFRLHEAHWVNPFLKALKLITFAESLGGTESFIT
YPATQTHMDIPEEERIARGVCNCLLRFSVGLENVEDIKADLLQAFAQLN
>Mature_368_residues
TQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLEETIAKLEGGERGFACASGMAA
IQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVKPVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAAI
AKIAKKHNLLLIVDNTFLTPVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDSW
LTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYPNKGGMLSFRLHEAHWVNPFLKALKLITFAESLGGTESFITY
PATQTHMDIPEEERIARGVCNCLLRFSVGLENVEDIKADLLQAFAQLN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=367, Percent_Identity=39.7820163487738, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI299473759, Length=367, Percent_Identity=35.6948228882834, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI25453487, Length=366, Percent_Identity=35.2459016393443, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=38.7862796833773, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=27.5689223057644, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=347, Percent_Identity=42.0749279538905, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=40.9221902017291, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71980708, Length=378, Percent_Identity=34.1269841269841, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=380, Percent_Identity=38.4210526315789, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=31.8518518518519, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=32.1100917431193, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=281, Percent_Identity=34.5195729537367, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=387, Percent_Identity=26.0981912144703, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=38.6058981233244, Blast_Score=271, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40566; Mature: 40435

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCCCHHHHHHH
ETIAKLEGGERGFACASGMAAIQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHCCCCCCC
PVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAAIAKIAKKHNLLLIVDNTFLT
EEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHCCEEEEECCCHHH
PVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDS
HHHHHHHCCCCCEEEECCCEEECCCCCCEEEEEEECCHHHHHHHHHHCCCCCCEECCCHH
WLTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYPNKGGMLSFRLHEAHWVNPFL
HEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCHHHHHHH
KALKLITFAESLGGTESFITYPATQTHMDIPEEERIARGVCNCLLRFSVGLENVEDIKAD
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLQAFAQLN
HHHHHHHCC
>Mature Secondary Structure 
TQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLE
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCCCHHHHHHH
ETIAKLEGGERGFACASGMAAIQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHCCCCCCC
PVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAAIAKIAKKHNLLLIVDNTFLT
EEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHCCEEEEECCCHHH
PVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDS
HHHHHHHCCCCCEEEECCCEEECCCCCCEEEEEEECCHHHHHHHHHHCCCCCCEECCCHH
WLTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYPNKGGMLSFRLHEAHWVNPFL
HEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCHHHHHHH
KALKLITFAESLGGTESFITYPATQTHMDIPEEERIARGVCNCLLRFSVGLENVEDIKAD
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLQAFAQLN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]