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The map label for this gene is rnt

Identifier: 15602853

GI number: 15602853

Start: 1159095

End: 1159757

Strand: Direct

Name: rnt

Synonym: PM0988

Alternate gene names: 15602853

Gene position: 1159095-1159757 (Clockwise)

Preceding gene: 15602852

Following gene: 15602854

Centisome position: 51.34

GC content: 41.78

Gene sequence:

>663_bases
GTGACAGAAATACAAGCTGAAGCAACAAATACCCATTTATTAAAACATCGTTTTCGCGGTTATTTCCCGGTTATTATTGA
TGTTGAAACTGCAGGGTTCAATGCGAAAACTGACGCATTACTTGAACTTGCCGCCATTACAGTCAAAATGAACGAAGACG
GTCTTTTAGTCCCCGATCAAACTTGCCATTTTCACATTGAACCTTTTGCGGGTGCTAATATTAACCCCGAGTCACTCCAG
TTTAATGGGATTGATATTCACAATCCATTACGAGGGGCAGTCAGTGAAACAGTCGCTATCACAGAGCTCTTCAAAATGGT
TCGTAAAGCACAAAAAGCAGCAGACTGCCAACGCTCTATTATCGTGGCACACAATGCGGCCTTTGATCAAAGTTTCTTAA
TGGCCGCAGCAGAACGAACAGGCGTCAAACGCAATCCATTTCATCCTTTTGGTATGTTTGATACCGCAACGTTAAGTGGT
TTTATGTTTGGACAAACCGTCTTGGTTAAAGCTTGCCAAGCCGCTCAAATTGCTTTTGATGGTAAACAGGCCCATTCTGC
GTTATACGATACAGAGCGGACTGCTGAATTGTTCTGTTATATGGTGAATCATTTAAAACATTTAGGTGGTTTTCCACACC
AACAAGCTAACGAAAAGAAATGA

Upstream 100 bases:

>100_bases
AGGTAATTAAGTTTCTCGCTCATGAAGTGCGGTCAATTTTAAAAAAATTTCTATAGCGTGGCATTTGTCCACGCTATATT
ATTTCAAATAGGATAATAAG

Downstream 100 bases:

>100_bases
TTATTCTGCATAAAAAATCTGTTTTATCACCATTTTTATGCAAAAAGATAGACAAATTATTTTTTTTAAGTAAATTTAGA
ACTCTTTTAAAAAACATAAC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTEIQAEATNTHLLKHRFRGYFPVIIDVETAGFNAKTDALLELAAITVKMNEDGLLVPDQTCHFHIEPFAGANINPESLQ
FNGIDIHNPLRGAVSETVAITELFKMVRKAQKAADCQRSIIVAHNAAFDQSFLMAAAERTGVKRNPFHPFGMFDTATLSG
FMFGQTVLVKACQAAQIAFDGKQAHSALYDTERTAELFCYMVNHLKHLGGFPHQQANEKK

Sequences:

>Translated_220_residues
MTEIQAEATNTHLLKHRFRGYFPVIIDVETAGFNAKTDALLELAAITVKMNEDGLLVPDQTCHFHIEPFAGANINPESLQ
FNGIDIHNPLRGAVSETVAITELFKMVRKAQKAADCQRSIIVAHNAAFDQSFLMAAAERTGVKRNPFHPFGMFDTATLSG
FMFGQTVLVKACQAAQIAFDGKQAHSALYDTERTAELFCYMVNHLKHLGGFPHQQANEKK
>Mature_219_residues
TEIQAEATNTHLLKHRFRGYFPVIIDVETAGFNAKTDALLELAAITVKMNEDGLLVPDQTCHFHIEPFAGANINPESLQF
NGIDIHNPLRGAVSETVAITELFKMVRKAQKAADCQRSIIVAHNAAFDQSFLMAAAERTGVKRNPFHPFGMFDTATLSGF
MFGQTVLVKACQAAQIAFDGKQAHSALYDTERTAELFCYMVNHLKHLGGFPHQQANEKK

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=205, Percent_Identity=67.3170731707317, Blast_Score=293, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_PASMU (P57896)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245925.1
- ProteinModelPortal:   P57896
- SMR:   P57896
- GeneID:   1244335
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0988
- NMPDR:   fig|272843.1.peg.988
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- BioCyc:   PMUL272843:PM0988-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24349; Mature: 24218

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIQAEATNTHLLKHRFRGYFPVIIDVETAGFNAKTDALLELAAITVKMNEDGLLVPDQ
CCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHEEEEEEEEECCCCEEECCC
TCHFHIEPFAGANINPESLQFNGIDIHNPLRGAVSETVAITELFKMVRKAQKAADCQRSI
CEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IVAHNAAFDQSFLMAAAERTGVKRNPFHPFGMFDTATLSGFMFGQTVLVKACQAAQIAFD
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEC
GKQAHSALYDTERTAELFCYMVNHLKHLGGFPHQQANEKK
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TEIQAEATNTHLLKHRFRGYFPVIIDVETAGFNAKTDALLELAAITVKMNEDGLLVPDQ
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHEEEEEEEEECCCCEEECCC
TCHFHIEPFAGANINPESLQFNGIDIHNPLRGAVSETVAITELFKMVRKAQKAADCQRSI
CEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IVAHNAAFDQSFLMAAAERTGVKRNPFHPFGMFDTATLSGFMFGQTVLVKACQAAQIAFD
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEC
GKQAHSALYDTERTAELFCYMVNHLKHLGGFPHQQANEKK
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100