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The map label for this gene is gloA [H]

Identifier: 15602852

GI number: 15602852

Start: 1158597

End: 1159004

Strand: Direct

Name: gloA [H]

Synonym: PM0987

Alternate gene names: 15602852

Gene position: 1158597-1159004 (Clockwise)

Preceding gene: 15602850

Following gene: 15602853

Centisome position: 51.32

GC content: 41.42

Gene sequence:

>408_bases
ATGCGAATTTTACATACAATGTTGCGCGTAACAAATTTAGAGCGTTCAATCCAGTTTTACCAACAGGTTTTAGGTATGCG
TTTACTGCGTACAAGTGACAATCCGGAGTATAAATATACGTTGGCATTTCTTGGTTATGACGATGAAGAGAATGCCTCCG
TATTAGAATTAACCTATAACTGGGGCGTGACTGAATACGAACTGGGTACGGCTTATGGTCATATTGCGATTGGCGTTGAG
GATATTTATGCAACCTGTGATGCGGTACGACAAGCAGGCGGTAAAATCACCCGTGAGCCCGGTCCAGTGAAAGGTGGAAA
AACAGTGATTGCGTTTGTCGAAGATCCTGATGGTTACAAAATCGAATTTATTGAAAATAAACATGCGCAATCTGGCTTAG
GTAATTAA

Upstream 100 bases:

>100_bases
AGTTAGAGAACATAATGATTTTTCACGATGATTATGGCATAATCATTTCCAATTTTGGCAAAAATTTTTCAGGTTCATAA
AAATCAAAATGGGGGGGATA

Downstream 100 bases:

>100_bases
GTTTCTCGCTCATGAAGTGCGGTCAATTTTAAAAAAATTTCTATAGCGTGGCATTTGTCCACGCTATATTATTTCAAATA
GGATAATAAGGTGACAGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRILHTMLRVTNLERSIQFYQQVLGMRLLRTSDNPEYKYTLAFLGYDDEENASVLELTYNWGVTEYELGTAYGHIAIGVE
DIYATCDAVRQAGGKITREPGPVKGGKTVIAFVEDPDGYKIEFIENKHAQSGLGN

Sequences:

>Translated_135_residues
MRILHTMLRVTNLERSIQFYQQVLGMRLLRTSDNPEYKYTLAFLGYDDEENASVLELTYNWGVTEYELGTAYGHIAIGVE
DIYATCDAVRQAGGKITREPGPVKGGKTVIAFVEDPDGYKIEFIENKHAQSGLGN
>Mature_135_residues
MRILHTMLRVTNLERSIQFYQQVLGMRLLRTSDNPEYKYTLAFLGYDDEENASVLELTYNWGVTEYELGTAYGHIAIGVE
DIYATCDAVRQAGGKITREPGPVKGGKTVIAFVEDPDGYKIEFIENKHAQSGLGN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=36.1842105263158, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=69.6296296296296, Blast_Score=201, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=43.1654676258993, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129743, Length=151, Percent_Identity=36.4238410596026, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920404, Length=145, Percent_Identity=28.2758620689655, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15167; Mature: 15167

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVTNLERSIQFYQQVLGMRLLRTSDNPEYKYTLAFLGYDDEENASVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGVTEYELGTAYGHIAIGVEDIYATCDAVRQAGGKITREPGPVKGGKTVIAFVEDPDGYK
CCCEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEECCCCCE
IEFIENKHAQSGLGN
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRILHTMLRVTNLERSIQFYQQVLGMRLLRTSDNPEYKYTLAFLGYDDEENASVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGVTEYELGTAYGHIAIGVEDIYATCDAVRQAGGKITREPGPVKGGKTVIAFVEDPDGYK
CCCEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEECCCCCE
IEFIENKHAQSGLGN
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]