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The map label for this gene is aspS

Identifier: 15602848

GI number: 15602848

Start: 1152672

End: 1154438

Strand: Reverse

Name: aspS

Synonym: PM0983

Alternate gene names: 15602848

Gene position: 1154438-1152672 (Counterclockwise)

Preceding gene: 15602851

Following gene: 15602847

Centisome position: 51.14

GC content: 41.37

Gene sequence:

>1767_bases
ATGATGCGTACACATTATTGCGGTGCATTAAACCGTGACAATATCGGACAAGAAGTAACATTGAGTGGTTGGGTGCATCG
TCGCCGTGATTTAGGTGGGTTGATTTTTATTGATATGCGTGATCGTGAAGGGATTGTACAAGTGTGTTTCGATCCAGTAT
ATCAAGAAGCACTCACCACAGCAGCAAGTTTACGTAATGAGTTTTGTATTCAAATTAAAGGCGAAGTGATTGCCCGCCCA
GATAATCAAATCAACAAAAATATGGCAACAGGCGAAGTAGAAGTGTTAGCAAAATCCCTGTCTATTTATAACAGTGCAGA
GCCATTACCTCTCGACTTTAACCAAAATAATACAGAAGAACAGCGTTTAAAATACCGTTATTTAGACTTACGTCGCCCAG
AAATGGCGCAACGTTTAAAAACACGAGCCAAAATTACCAGCTTTGTGCGTCGCTTTATGGACGAACATGGTTTCTTAGAT
ATTGAAACACCGATGTTAACCAAAGCAACGCCGGAAGGAGCTCGTGACTATTTAGTACCAAGCCGAGTACATAAAGGTAA
ATTCTATGCATTACCGCAATCACCACAGTTATTTAAACAATTGTTAATGATGTCAGGGTTTGACCGTTATTATCAAATTG
TGAAATGTTTCCGTGATGAAGATTTGCGTGCGGATCGTCAACCTGAATTCACTCAAATCGATGTAGAAACCTCTTTCTTA
ACGGCACCAGAAGTGCGTGAGATAATGGAAAAAATGGTACATGGTTTGTGGCGGGAAACCCTTGGGGTAGATTTAGGTAA
ATTCCCTGTGATGACCTTTGCAGAAGCGATGCGTCGTTTTGGCTCAGATAAACCTGATTTACGTAATCCATTAGAAATGG
TGGACGTAGCGGATTTATTAAAAGAGGTTGAGTTTAAAGTATTCAATGGTCCAGCGAATGACCCTAATGGTCGTATTGCC
GTCATTCGTGTACCAAATGGCACAGACATTACGCGTAAACAAATTGATGAATACACGCAATTTGTTGGGATTTATGGGGC
AAAAGGTTTGGCTTGGTTAAAAGTCAATGATCTTGCTGCGGGGATGGATGGCGTACAAAGTCCAATCGCAAAATTTTTAT
CTGAAGCGGTGTTAAAAGCCCTTTTAGAGCGTGTACAAGCACAAAACGGCGATATTCTTTTCTTTGGTGCCGATAACTAT
AAAGTAACAACTGATGCAATGGGCGCGTTACGTTTGAAGTTAGGACGTGATTTGGGCTTAACGAAATTAGAAGAATGGCA
ACCACTTTGGGTGATTGATTTCCCAATGTTTGAGCGTGATGATGAAGGTGATCTTGCCGCTATGCACCACCCATTTACTT
CACCAAAAGATTTCAGCCCAGAACAATTAGAAGCGGATCCAACTTCAGCCGTTGCTAATGCTTACGATATGGTGATTAAT
GGTTATGAAGTGGGCGGGGGATCGGTGCGTATTTTCGATCCGAAAATGCAACAAACGGTATTCCGTATTTTAGGGATTAA
TGAAGCAGAACAACGTGAAAAATTCGGTTTCTTATTAGATGCGTTAAAATTTGGTACACCACCACATGCAGGCTTGGCTT
TTGGTTTAGATCGTTTAACGATGCTATTGACTGGAACGGACAATATCCGTGATGTGATTGCGTTTCCGAAAACCACAGCC
GCCGCCTGTTTAATGACAGAGGCGCCGAGTTTTGCAAACCCACAAGCATTGAATGAACTGTCAGTTCAAGTAATTAAATC
TGAATAA

Upstream 100 bases:

>100_bases
TGGTTGATTTGTAAGCATAAATAACCTTTTTATCTCTCTTGGTTTTCCGCTATAATTGAGTGAATTTTTGAGCATAATTA
AGAGTACAAAGGATTTTGAA

Downstream 100 bases:

>100_bases
AGTTTATAAAAGGTGCGGTTATGATAACTGCACCTTTTCATGTAAATTTATTGGGAGGTAATTATGCGTTATGCTATCTT
ATTGATTCCTATTTTTTTAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCFDPVYQEALTTAASLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKSLSIYNSAEPLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDEHGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMEKMVHGLWRETLGVDLGKFPVMTFAEAMRRFGSDKPDLRNPLEMVDVADLLKEVEFKVFNGPANDPNGRIA
VIRVPNGTDITRKQIDEYTQFVGIYGAKGLAWLKVNDLAAGMDGVQSPIAKFLSEAVLKALLERVQAQNGDILFFGADNY
KVTTDAMGALRLKLGRDLGLTKLEEWQPLWVIDFPMFERDDEGDLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTA
AACLMTEAPSFANPQALNELSVQVIKSE

Sequences:

>Translated_588_residues
MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCFDPVYQEALTTAASLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKSLSIYNSAEPLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDEHGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMEKMVHGLWRETLGVDLGKFPVMTFAEAMRRFGSDKPDLRNPLEMVDVADLLKEVEFKVFNGPANDPNGRIA
VIRVPNGTDITRKQIDEYTQFVGIYGAKGLAWLKVNDLAAGMDGVQSPIAKFLSEAVLKALLERVQAQNGDILFFGADNY
KVTTDAMGALRLKLGRDLGLTKLEEWQPLWVIDFPMFERDDEGDLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTA
AACLMTEAPSFANPQALNELSVQVIKSE
>Mature_588_residues
MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCFDPVYQEALTTAASLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKSLSIYNSAEPLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDEHGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMEKMVHGLWRETLGVDLGKFPVMTFAEAMRRFGSDKPDLRNPLEMVDVADLLKEVEFKVFNGPANDPNGRIA
VIRVPNGTDITRKQIDEYTQFVGIYGAKGLAWLKVNDLAAGMDGVQSPIAKFLSEAVLKALLERVQAQNGDILFFGADNY
KVTTDAMGALRLKLGRDLGLTKLEEWQPLWVIDFPMFERDDEGDLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTA
AACLMTEAPSFANPQALNELSVQVIKSE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=37.7483443708609, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=250, Percent_Identity=28.8, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI194272210, Length=250, Percent_Identity=28.8, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI45439306, Length=253, Percent_Identity=25.6916996047431, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI226491502, Length=263, Percent_Identity=21.6730038022814, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=77.4358974358974, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=27.5510204081633, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=27.4576271186441, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=33.6120401337793, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17551876, Length=297, Percent_Identity=26.2626262626263, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=251, Percent_Identity=23.5059760956175, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=251, Percent_Identity=22.3107569721116, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=251, Percent_Identity=22.3107569721116, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=633, Percent_Identity=33.4913112164297, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=28, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=186, Percent_Identity=27.9569892473118, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24584738, Length=609, Percent_Identity=34.9753694581281, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=29.0196078431373, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=29.0196078431373, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=26.3598326359833, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921528, Length=244, Percent_Identity=27.0491803278689, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PASMU (P57895)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245920.1
- ProteinModelPortal:   P57895
- SMR:   P57895
- PRIDE:   P57895
- GeneID:   1244330
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0983
- NMPDR:   fig|272843.1.peg.983
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   PMUL272843:PM0983-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66434; Mature: 66434

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCFDPVYQEALTT
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
AASLRNEFCIQIKGEVIARPDNQINKNMATGEVEVLAKSLSIYNSAEPLPLDFNQNNTEE
HHHHCCCEEEEECCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDEHGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMEKMVHGLWRETLGVDLGKFPVMTFAEAMRRFGSDKPDLRNPLEMVDVADLL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KEVEFKVFNGPANDPNGRIAVIRVPNGTDITRKQIDEYTQFVGIYGAKGLAWLKVNDLAA
HHCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHH
GMDGVQSPIAKFLSEAVLKALLERVQAQNGDILFFGADNYKVTTDAMGALRLKLGRDLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCC
TKLEEWQPLWVIDFPMFERDDEGDLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLAFGLDRLT
CEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHE
MLLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPQALNELSVQVIKSE
EEEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHEEEECC
>Mature Secondary Structure
MMRTHYCGALNRDNIGQEVTLSGWVHRRRDLGGLIFIDMRDREGIVQVCFDPVYQEALTT
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
AASLRNEFCIQIKGEVIARPDNQINKNMATGEVEVLAKSLSIYNSAEPLPLDFNQNNTEE
HHHHCCCEEEEECCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDEHGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMEKMVHGLWRETLGVDLGKFPVMTFAEAMRRFGSDKPDLRNPLEMVDVADLL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KEVEFKVFNGPANDPNGRIAVIRVPNGTDITRKQIDEYTQFVGIYGAKGLAWLKVNDLAA
HHCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHH
GMDGVQSPIAKFLSEAVLKALLERVQAQNGDILFFGADNYKVTTDAMGALRLKLGRDLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCC
TKLEEWQPLWVIDFPMFERDDEGDLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGINEAEQREKFGFLLDALKFGTPPHAGLAFGLDRLT
CEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHE
MLLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPQALNELSVQVIKSE
EEEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100