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The map label for this gene is ruvC

Identifier: 15602843

GI number: 15602843

Start: 1149417

End: 1149989

Strand: Reverse

Name: ruvC

Synonym: PM0978

Alternate gene names: 15602843

Gene position: 1149989-1149417 (Counterclockwise)

Preceding gene: 15602844

Following gene: 15602842

Centisome position: 50.94

GC content: 42.41

Gene sequence:

>573_bases
ATGGCAATTATTTTAGGCATCGATCCTGGCTCTCGCGTAACTGGCTATGGCGTTATACGTCAAGCGGGTAGGCATTTAGA
ATATTTAGGTAGTGGCGTGATCCGGACGTCTGTTACAGATTTACCAACTCGTTTGAAACGGATCTACATGGGGGTAAATG
AAATTATTTTGCAATATCAACCGGATATGTTTGCTATTGAAGAAGTGTTTTTAGCCAAAAATGCCAATTCAGCTTTAAAA
CTTGGGCAAGCAAGAGGTGCCGCGATTGTTGCCGCAGTTAATCATGATTTACCCGTTTTCGAATATGCGGCGCGCTTAGT
CAAACAAACGGTAGTAGGGATTGGTTCTGCAGATAAAATTCAAGTACAAGATATGGTAACACGGATTTTAACTTTATCTG
AAAAACCACAAGCCGATGCGGCGGATGCGTTAGCGATAGCGATTACGCATGCCCATTCTCTTCAACACGCTTTCCATGTT
ACAAATAGTGCACAAGCGACAGAAAAACCAGAAAAAACGACCGCACTGCTTAAAGCGCGATACAGTCGAGGACGCTTTCG
TTTAAAAATTTAA

Upstream 100 bases:

>100_bases
TTGTACTAACGCAAAATAATGTGTGTTAACAATTTTTCGGCATAGATGAAAATAATGGATTTACAGTAAAATGTAGATCC
ATTTATTTTTGGGGCAAGTA

Downstream 100 bases:

>100_bases
CTGGATGTTCATCCAGTTTTATTTTATGCTATACAACAAAATAAATGATGTGATGAGGAAGATATGATTGGTCATTTGAC
GGGACGTTTAGTTGAAAAAC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAIILGIDPGSRVTGYGVIRQAGRHLEYLGSGVIRTSVTDLPTRLKRIYMGVNEIILQYQPDMFAIEEVFLAKNANSALK
LGQARGAAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKIQVQDMVTRILTLSEKPQADAADALAIAITHAHSLQHAFHV
TNSAQATEKPEKTTALLKARYSRGRFRLKI

Sequences:

>Translated_190_residues
MAIILGIDPGSRVTGYGVIRQAGRHLEYLGSGVIRTSVTDLPTRLKRIYMGVNEIILQYQPDMFAIEEVFLAKNANSALK
LGQARGAAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKIQVQDMVTRILTLSEKPQADAADALAIAITHAHSLQHAFHV
TNSAQATEKPEKTTALLKARYSRGRFRLKI
>Mature_189_residues
AIILGIDPGSRVTGYGVIRQAGRHLEYLGSGVIRTSVTDLPTRLKRIYMGVNEIILQYQPDMFAIEEVFLAKNANSALKL
GQARGAAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKIQVQDMVTRILTLSEKPQADAADALAIAITHAHSLQHAFHVT
NSAQATEKPEKTTALLKARYSRGRFRLKI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=163, Percent_Identity=76.0736196319018, Blast_Score=226, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PASMU (P57894)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245915.1
- ProteinModelPortal:   P57894
- SMR:   P57894
- GeneID:   1244325
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0978
- NMPDR:   fig|272843.1.peg.978
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- BioCyc:   PMUL272843:PM0978-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 20702; Mature: 20571

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIILGIDPGSRVTGYGVIRQAGRHLEYLGSGVIRTSVTDLPTRLKRIYMGVNEIILQYQ
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHEECC
PDMFAIEEVFLAKNANSALKLGQARGAAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKI
CCHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCE
QVQDMVTRILTLSEKPQADAADALAIAITHAHSLQHAFHVTNSAQATEKPEKTTALLKAR
EHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YSRGRFRLKI
HCCCEEEEEC
>Mature Secondary Structure 
AIILGIDPGSRVTGYGVIRQAGRHLEYLGSGVIRTSVTDLPTRLKRIYMGVNEIILQYQ
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHEECC
PDMFAIEEVFLAKNANSALKLGQARGAAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKI
CCHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCE
QVQDMVTRILTLSEKPQADAADALAIAITHAHSLQHAFHVTNSAQATEKPEKTTALLKAR
EHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YSRGRFRLKI
HCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100