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The map label for this gene is ruvB

Identifier: 15602841

GI number: 15602841

Start: 1147713

End: 1148720

Strand: Reverse

Name: ruvB

Synonym: PM0976

Alternate gene names: 15602841

Gene position: 1148720-1147713 (Counterclockwise)

Preceding gene: 15602842

Following gene: 15602839

Centisome position: 50.88

GC content: 41.47

Gene sequence:

>1008_bases
ATGATTGAAGCAGATAGAATTATTAGTACAAGTGCGAAAGGCGATGAAGAGTATATTGATCGTGCGATTCGTCCAAAATT
GCTAAATGATTATGTTGGACAACCGCACGTCTGCGAACAAATGGCGATTTTTATTCAAGCGGCTAAATTGCGCCAAGATG
CTTTGGATCATTTATTGATTTTTGGTCCACCTGGGTTAGGTAAAACCACATTAGCCAATATTGTAGCGCATGAAATGGGT
GTGAATATTCGTACTACTTCAGGACCAGTATTAGAAAAAGCGGGCGATCTTGCCGCAATGCTTACGAATTTAGAACCACA
TGATGTGCTATTTATTGATGAAATTCATCGCTTATCCCCTGCAATTGAAGAAGTGTTATATCCAGCGATGGAAGATTATC
AATTAGATATTATGATAGGTGAAGGACCTGCGGCTCGTTCAATCAAGTTAGATCTTCCACCTTTTACATTAATTGGGGCA
ACAACACGTGCGGGCTCGTTAACATCGCCGTTGCGTGATCGTTTTGGGATTGTACAACGGCTGGAGTTTTATGCAGTGGA
AGATTTAACTTCGATCGTAGCGCGTAGTGCAAATTGTTTGAATTTATCTATTTCCGATACCGCGTGTTATGAAATTGCAC
GTCGTTCAAGGGGGACACCTCGAATTGCTAATCGTTTATTAAGACGCGTGAGAGACTTTGCAGATGTCCGTCATGCCGGC
ATTATTTCAGAAGAAAGTGCTAAAGCCGCATTGTTAATGTTGGATGTGGATGATGCAGGTTTTGATTATTTGGATCGCAA
ACTGTTAAATGCTGTGATTGAACGTTTTGACGGGGGACCTGTAGGATTGGATAACCTTGCTGCTGCGATTGGAGAAGAAC
GAGAAACGATAGAAGATGTGCTAGAGCCTTATTTAATACAGCAAGGCTTTTTACAGCGTACACCTCGAGGACGTATGGCA
ACCTCAAGAACGTACCGTTATTTTGGGTTAGAGAAATTAACTGAATAA

Upstream 100 bases:

>100_bases
TGAAAAACTGGTGAAAAAAATCAGCAAGCCGGCACTTTCTAGTGAGCAACTTATCCGTGAAGCATTAAAAGCCGCCTTAT
AATCTTTAAGTGAAAAGAAC

Downstream 100 bases:

>100_bases
GACGAGCGGAGCATTATTAAAAAAGCGTGTTAATGAAAAATTAACACGCTTTTTTAGTTTATTGCTGTTTGAGAGTATTT
AACTTAGTTAAAGCAAAATC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MIEADRIISTSAKGDEEYIDRAIRPKLLNDYVGQPHVCEQMAIFIQAAKLRQDALDHLLIFGPPGLGKTTLANIVAHEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYAVEDLTSIVARSANCLNLSISDTACYEIARRSRGTPRIANRLLRRVRDFADVRHAG
IISEESAKAALLMLDVDDAGFDYLDRKLLNAVIERFDGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TSRTYRYFGLEKLTE

Sequences:

>Translated_335_residues
MIEADRIISTSAKGDEEYIDRAIRPKLLNDYVGQPHVCEQMAIFIQAAKLRQDALDHLLIFGPPGLGKTTLANIVAHEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYAVEDLTSIVARSANCLNLSISDTACYEIARRSRGTPRIANRLLRRVRDFADVRHAG
IISEESAKAALLMLDVDDAGFDYLDRKLLNAVIERFDGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TSRTYRYFGLEKLTE
>Mature_335_residues
MIEADRIISTSAKGDEEYIDRAIRPKLLNDYVGQPHVCEQMAIFIQAAKLRQDALDHLLIFGPPGLGKTTLANIVAHEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYAVEDLTSIVARSANCLNLSISDTACYEIARRSRGTPRIANRLLRRVRDFADVRHAG
IISEESAKAALLMLDVDDAGFDYLDRKLLNAVIERFDGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TSRTYRYFGLEKLTE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=80.6060606060606, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787119, Length=232, Percent_Identity=26.7241379310345, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_PASMU (P57892)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245913.1
- ProteinModelPortal:   P57892
- SMR:   P57892
- GeneID:   1244323
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0976
- NMPDR:   fig|272843.1.peg.976
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   PMUL272843:PM0976-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37180; Mature: 37180

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRIISTSAKGDEEYIDRAIRPKLLNDYVGQPHVCEQMAIFIQAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
FGPPGLGKTTLANIVAHEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
LEFYAVEDLTSIVARSANCLNLSISDTACYEIARRSRGTPRIANRLLRRVRDFADVRHAG
HHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
IISEESAKAALLMLDVDDAGFDYLDRKLLNAVIERFDGGPVGLDNLAAAIGEERETIEDV
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFLQRTPRGRMATSRTYRYFGLEKLTE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHCC
>Mature Secondary Structure
MIEADRIISTSAKGDEEYIDRAIRPKLLNDYVGQPHVCEQMAIFIQAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
FGPPGLGKTTLANIVAHEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
LEFYAVEDLTSIVARSANCLNLSISDTACYEIARRSRGTPRIANRLLRRVRDFADVRHAG
HHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
IISEESAKAALLMLDVDDAGFDYLDRKLLNAVIERFDGGPVGLDNLAAAIGEERETIEDV
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFLQRTPRGRMATSRTYRYFGLEKLTE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100