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The map label for this gene is eda [H]

Identifier: 15602812

GI number: 15602812

Start: 1117632

End: 1118294

Strand: Reverse

Name: eda [H]

Synonym: PM0947

Alternate gene names: 15602812

Gene position: 1118294-1117632 (Counterclockwise)

Preceding gene: 15602813

Following gene: 15602811

Centisome position: 49.54

GC content: 44.49

Gene sequence:

>663_bases
ATGGCTTACCGCACCGCAGAGATCGTTGAAAAATTACGCGCTTGGAAAGTCATTCCTGTGATTGCGTTAGATAATCCGGA
GACGATTTTACCGCTAGCAGATTGTTTAGCTGAACATGGTTTACCTGTCGTAGAAATCACGTTTCGTTCAAGTGCAGCGG
AACAAGCTATTCGTTTACTTAAACAACAACGCCCAGCTATTTTTGTCGCGGCTGGCACGGTGCTTAACCCAGAACAAGTG
ATGCAAGCCAAGAATGCGGGGGCAGATTGTGTGGTGACACCGGGATTTAATCCAAAAATTGTTGAACTGTGTCAACGTTT
ATCCTTCCCCATTATCCCCGGCGTGAATAACCCAATGGCAATTGAAGCTGCGCTTGAAATGGGCATTCATGCTGTAAAAT
TTTTCCCCGCGGAAGCTTCGGGCGGGGTGGCAATGATTAAAGCATTGTTAGGACCTTATCCGATGTTACAAATTATGCCA
ACAGGGGGCATTCATTTACAAAATATCTGTGATTATTTGGCAATTCCTAATGTGGTCGCTTGTGGTGGTTCTTGGTTTGT
AGAAAAACAGCTCATTCAACAACAAAATTGGCAAGAAATTGGGCGTTTGACACAAGCGGTGATGGCACAGATAAACAACG
AAAAAGGGCGGTCATTTTTGTGA

Upstream 100 bases:

>100_bases
TGAAATTCAGAAAATTAATTTTGCTTCGGATTTGTCAGATGCTTTTAATAAAATCCGCGTTTTTGAACAACAAGCATAAT
ACGGTTTAGAGGAGAAAAAC

Downstream 100 bases:

>100_bases
AAATTTTTTCACTTTGGGTTTGTGTGAATCTTTTATAAGATAAACAGTCTAATGGAGTTCTTATCGGTACTTTATGAGGA
AAGCTAACATGAATTTTAAT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAYRTAEIVEKLRAWKVIPVIALDNPETILPLADCLAEHGLPVVEITFRSSAAEQAIRLLKQQRPAIFVAAGTVLNPEQV
MQAKNAGADCVVTPGFNPKIVELCQRLSFPIIPGVNNPMAIEAALEMGIHAVKFFPAEASGGVAMIKALLGPYPMLQIMP
TGGIHLQNICDYLAIPNVVACGGSWFVEKQLIQQQNWQEIGRLTQAVMAQINNEKGRSFL

Sequences:

>Translated_220_residues
MAYRTAEIVEKLRAWKVIPVIALDNPETILPLADCLAEHGLPVVEITFRSSAAEQAIRLLKQQRPAIFVAAGTVLNPEQV
MQAKNAGADCVVTPGFNPKIVELCQRLSFPIIPGVNNPMAIEAALEMGIHAVKFFPAEASGGVAMIKALLGPYPMLQIMP
TGGIHLQNICDYLAIPNVVACGGSWFVEKQLIQQQNWQEIGRLTQAVMAQINNEKGRSFL
>Mature_219_residues
AYRTAEIVEKLRAWKVIPVIALDNPETILPLADCLAEHGLPVVEITFRSSAAEQAIRLLKQQRPAIFVAAGTVLNPEQVM
QAKNAGADCVVTPGFNPKIVELCQRLSFPIIPGVNNPMAIEAALEMGIHAVKFFPAEASGGVAMIKALLGPYPMLQIMPT
GGIHLQNICDYLAIPNVVACGGSWFVEKQLIQQQNWQEIGRLTQAVMAQINNEKGRSFL

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=179, Percent_Identity=35.7541899441341, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23938; Mature: 23807

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYRTAEIVEKLRAWKVIPVIALDNPETILPLADCLAEHGLPVVEITFRSSAAEQAIRLL
CCCCHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
KQQRPAIFVAAGTVLNPEQVMQAKNAGADCVVTPGFNPKIVELCQRLSFPIIPGVNNPMA
HHCCCEEEEEECCEECHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCHH
IEAALEMGIHAVKFFPAEASGGVAMIKALLGPYPMLQIMPTGGIHLQNICDYLAIPNVVA
HHHHHHHCHHHEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCEEE
CGGSWFVEKQLIQQQNWQEIGRLTQAVMAQINNEKGRSFL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYRTAEIVEKLRAWKVIPVIALDNPETILPLADCLAEHGLPVVEITFRSSAAEQAIRLL
CCCHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
KQQRPAIFVAAGTVLNPEQVMQAKNAGADCVVTPGFNPKIVELCQRLSFPIIPGVNNPMA
HHCCCEEEEEECCEECHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCHH
IEAALEMGIHAVKFFPAEASGGVAMIKALLGPYPMLQIMPTGGIHLQNICDYLAIPNVVA
HHHHHHHCHHHEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCEEE
CGGSWFVEKQLIQQQNWQEIGRLTQAVMAQINNEKGRSFL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]