Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 15602810

GI number: 15602810

Start: 1115472

End: 1116851

Strand: Direct

Name: cysS

Synonym: PM0945

Alternate gene names: 15602810

Gene position: 1115472-1116851 (Clockwise)

Preceding gene: 15602806

Following gene: 15602818

Centisome position: 49.41

GC content: 40.36

Gene sequence:

>1380_bases
ATGCTAAAAATTTTTAATACATTAACAAGAGAAAAGGAAATCTTTAAACCCATTCATGCCAACAAAGTGGGTATGTATGT
TTGCGGTATTACGGTTTATGATTTATGCCACGTAGGACACGGGCGTACATTCGTTTGTTTTGATGTGATTGCTCGTTATC
TTCGGTATTTAGGCTATGACTTAACTTATGTGCGTAACATCACAGATGTGGACGATAAAATTATCAAACGGGCATTAGAA
AATAATGAGACATGTAATCAACTTGTTGAAAAAATGATTGCAGAAATGCACAAAGACTTTGATGCACTCAATGTATTACG
TCCAGATGTAGAACCCCGTGCAACCCACCATATTCCTGAAATTATCGCAATGATTGAAAAGCTGATAGCTCGTCAACATG
CTTATGTATCTGCTAATGGTGATGTGATGTTTGATGTCGAAAGTTTTAAAGAATACGGAAAGCTTTCTCGTCAAAATTTA
GAACAGCTCCAAGCAGGCGCTCGTGTTGAAATTGTTAATGTCAAAAAAAATCCGATGGATTTTGTGTTATGGAAAATGTC
CAAACCAGGAGAGCCAAGTTGGCCATCCCCTTGGGGAGAAGGTCGTCCCGGTTGGCATATTGAATGTTCTGCCATGAACC
ATAAAGAATTAGGTGAGCATTTTGATATTCATGGCGGAGGCTCGGATTTAACCTTCCCTCACCATGAAAATGAAATTGCA
CAGTCTTGCTGTGCCCATAGTGGACGCTACGTTAACTATTGGATACATTCTGGCATGATTATGGTTGATCGAGAAAAAAT
GTCCAAATCACTGGGTAATTTCTTTACATTACGTGAAGTATTATCACTCTATGACGCAGAAAGTGTACGTTACTTTCTAC
TGACAGCACATTACCGTAGCCAATTAAATTACAGCGAAGAAAACCTCAATCTTGCGCACAGTGCTTTAGAACGTTTATAT
ACGGCATTACGAGGAACAGACCCAACTGCAGTCGCGACAGAAGGGCAAAACTACCTTGCTGCCTTTAGAGAAGCCATGGA
TGATGATTTCAATACTCCGAAAGCCATTTCAGTCTTATTCGAAATCGCACGTGAAATCAACAAACTGAAAAATGAAGATA
TATTAAAAGCCAATGCGCTTGCAGCCCGCTTACGTGAATTAGCGGGAATATTAGGTTTACTCTACCAAGATCCAGAACAA
TTCTTACAATCGGGTTCAGACAATGATGAAGTCGCGCTGATTGAGGCGTTAATCAAACAGCGTAATGATGCCCGGGCAGC
AAAAGATTGGGCAAGTGCGGATGCCGCACGTAACAAACTGGCTGAAATGGGTGTGGTATTAGAAGATAACGTAAATGGTA
CCACTTGGCGTAAACAGTAA

Upstream 100 bases:

>100_bases
TTTCATAAAAGCGCATTAAATGCTATAAAGTAACTTTGTTTATGGCAAATTACGCTATGCTAAATTTATAACACTCCTAT
ACACTTAAGAGAACCAATCC

Downstream 100 bases:

>100_bases
GTCAAAAAATCATAAAAAATGGCAAGGCTCTCACCTTGCCATTTTTTTAACTAAAATCCAGCCTGTTTCTCAAGTTGTTC
CACAAGTGCATCATCTAAAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLKIFNTLTREKEIFKPIHANKVGMYVCGITVYDLCHVGHGRTFVCFDVIARYLRYLGYDLTYVRNITDVDDKIIKRALE
NNETCNQLVEKMIAEMHKDFDALNVLRPDVEPRATHHIPEIIAMIEKLIARQHAYVSANGDVMFDVESFKEYGKLSRQNL
EQLQAGARVEIVNVKKNPMDFVLWKMSKPGEPSWPSPWGEGRPGWHIECSAMNHKELGEHFDIHGGGSDLTFPHHENEIA
QSCCAHSGRYVNYWIHSGMIMVDREKMSKSLGNFFTLREVLSLYDAESVRYFLLTAHYRSQLNYSEENLNLAHSALERLY
TALRGTDPTAVATEGQNYLAAFREAMDDDFNTPKAISVLFEIAREINKLKNEDILKANALAARLRELAGILGLLYQDPEQ
FLQSGSDNDEVALIEALIKQRNDARAAKDWASADAARNKLAEMGVVLEDNVNGTTWRKQ

Sequences:

>Translated_459_residues
MLKIFNTLTREKEIFKPIHANKVGMYVCGITVYDLCHVGHGRTFVCFDVIARYLRYLGYDLTYVRNITDVDDKIIKRALE
NNETCNQLVEKMIAEMHKDFDALNVLRPDVEPRATHHIPEIIAMIEKLIARQHAYVSANGDVMFDVESFKEYGKLSRQNL
EQLQAGARVEIVNVKKNPMDFVLWKMSKPGEPSWPSPWGEGRPGWHIECSAMNHKELGEHFDIHGGGSDLTFPHHENEIA
QSCCAHSGRYVNYWIHSGMIMVDREKMSKSLGNFFTLREVLSLYDAESVRYFLLTAHYRSQLNYSEENLNLAHSALERLY
TALRGTDPTAVATEGQNYLAAFREAMDDDFNTPKAISVLFEIAREINKLKNEDILKANALAARLRELAGILGLLYQDPEQ
FLQSGSDNDEVALIEALIKQRNDARAAKDWASADAARNKLAEMGVVLEDNVNGTTWRKQ
>Mature_459_residues
MLKIFNTLTREKEIFKPIHANKVGMYVCGITVYDLCHVGHGRTFVCFDVIARYLRYLGYDLTYVRNITDVDDKIIKRALE
NNETCNQLVEKMIAEMHKDFDALNVLRPDVEPRATHHIPEIIAMIEKLIARQHAYVSANGDVMFDVESFKEYGKLSRQNL
EQLQAGARVEIVNVKKNPMDFVLWKMSKPGEPSWPSPWGEGRPGWHIECSAMNHKELGEHFDIHGGGSDLTFPHHENEIA
QSCCAHSGRYVNYWIHSGMIMVDREKMSKSLGNFFTLREVLSLYDAESVRYFLLTAHYRSQLNYSEENLNLAHSALERLY
TALRGTDPTAVATEGQNYLAAFREAMDDDFNTPKAISVLFEIAREINKLKNEDILKANALAARLRELAGILGLLYQDPEQ
FLQSGSDNDEVALIEALIKQRNDARAAKDWASADAARNKLAEMGVVLEDNVNGTTWRKQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=488, Percent_Identity=36.0655737704918, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI62240992, Length=265, Percent_Identity=41.1320754716981, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI21269877, Length=265, Percent_Identity=41.1320754716981, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI10835051, Length=265, Percent_Identity=41.1320754716981, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI303304987, Length=265, Percent_Identity=41.1320754716981, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=74.4034707158351, Blast_Score=710, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=436, Percent_Identity=32.3394495412844, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17509795, Length=436, Percent_Identity=32.3394495412844, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=328, Percent_Identity=35.3658536585366, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=37.2, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI19922364, Length=260, Percent_Identity=40, Blast_Score=189, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PASMU (P57890)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245882.1
- ProteinModelPortal:   P57890
- SMR:   P57890
- GeneID:   1244292
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0945
- NMPDR:   fig|272843.1.peg.945
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   PMUL272843:PM0945-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52272; Mature: 52272

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIFNTLTREKEIFKPIHANKVGMYVCGITVYDLCHVGHGRTFVCFDVIARYLRYLGYD
CCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCC
LTYVRNITDVDDKIIKRALENNETCNQLVEKMIAEMHKDFDALNVLRPDVEPRATHHIPE
HHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHH
IIAMIEKLIARQHAYVSANGDVMFDVESFKEYGKLSRQNLEQLQAGARVEIVNVKKNPMD
HHHHHHHHHHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCE
FVLWKMSKPGEPSWPSPWGEGRPGWHIECSAMNHKELGEHFDIHGGGSDLTFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCHHHHH
QSCCAHSGRYVNYWIHSGMIMVDREKMSKSLGNFFTLREVLSLYDAESVRYFLLTAHYRS
HHHHHCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
QLNYSEENLNLAHSALERLYTALRGTDPTAVATEGQNYLAAFREAMDDDFNTPKAISVLF
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHH
EIAREINKLKNEDILKANALAARLRELAGILGLLYQDPEQFLQSGSDNDEVALIEALIKQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHC
RNDARAAKDWASADAARNKLAEMGVVLEDNVNGTTWRKQ
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIFNTLTREKEIFKPIHANKVGMYVCGITVYDLCHVGHGRTFVCFDVIARYLRYLGYD
CCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCC
LTYVRNITDVDDKIIKRALENNETCNQLVEKMIAEMHKDFDALNVLRPDVEPRATHHIPE
HHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHH
IIAMIEKLIARQHAYVSANGDVMFDVESFKEYGKLSRQNLEQLQAGARVEIVNVKKNPMD
HHHHHHHHHHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCE
FVLWKMSKPGEPSWPSPWGEGRPGWHIECSAMNHKELGEHFDIHGGGSDLTFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCHHHHH
QSCCAHSGRYVNYWIHSGMIMVDREKMSKSLGNFFTLREVLSLYDAESVRYFLLTAHYRS
HHHHHCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
QLNYSEENLNLAHSALERLYTALRGTDPTAVATEGQNYLAAFREAMDDDFNTPKAISVLF
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHH
EIAREINKLKNEDILKANALAARLRELAGILGLLYQDPEQFLQSGSDNDEVALIEALIKQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHC
RNDARAAKDWASADAARNKLAEMGVVLEDNVNGTTWRKQ
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100