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The map label for this gene is purA [H]

Identifier: 15602803

GI number: 15602803

Start: 1107132

End: 1108430

Strand: Direct

Name: purA [H]

Synonym: PM0938

Alternate gene names: 15602803

Gene position: 1107132-1108430 (Clockwise)

Preceding gene: 15602802

Following gene: 15602804

Centisome position: 49.04

GC content: 43.34

Gene sequence:

>1299_bases
ATGGGAAAAAGTGTTGTTATCTTAGGTGCTCAATGGGGCGACGAAGGTAAAGGAAAAATTGTTGACTTATTAACTGACCG
AGTGAAATATGTGGTACGCTATCAAGGTGGTCATAATGCAGGTCACACCCTTATTATTAACGGTGAAAAAACCGTACTTC
GTTTAATTCCATCTGGTATTTTACGTGAAAATGTAACCTGTTTGATCGGCAATGGCGTTGTGCTTTCTCCTGCGGCATTA
ATGCAAGAAATGGGCGAACTGGAAAGCCGTGGTATTAATGTACGTGAACGTTTATTAATTTCAGAAGCCTGCCCTTTAAT
CCTACCTTATCATGTTGCGATGGATCATGCGCGTGAAGCCGCTTTAGGTAAAAACAAAATTGGAACAACCGGACGTGGTA
TTGGTCCTGCTTATGAAGATAAAGTCGCACGCCGTGGCTTACGTGTAGGTGACTTATTTAATCGTGAAGCCTTTGCTGAA
AAACTCAAAACGATCTTAGATTACTATAACTTCCAATTAGTCAATTACTATAAAGCCGAAGCCGTTGACTTCCAAAAAAC
CTTAGATGACGTTATGGCGGTCGCTGATATCATCACCGGTATGGTCGCTGATGTCACGACAATGCTAGATAAAGCTCGCA
AAAATGGCGACAATATCTTATTTGAAGGCGCACAAGGCACTATGTTAGACATTGACCACGGTACTTATCCGTTTGTGACC
AGTTCAAACACCACCGCAGGTGGCGTAGCAACCGGTGCCGGTTTTGGTCCTCGCAACTTAGATTATGTCTTAGGTATTAT
CAAAGCGTACTGTACACGTGTTGGTGGTGGACCATTCACTACGGAATTATTTGATGAAGTTGGTAGCGAAATTGCGCGGA
AAGGAAATGAATTTGGTGCCGTTACTGGTCGTCCACGTCGTTGTGGCTGGTTTGATGCAGTTGCGATTCGTCGCGCCATT
CAACTTAATTCAATTTCTGGTTTTTGTATGACCAAACTAGACGTGTTAGACGGCTTTGATGAAGTCAAAATCTGTGTCGC
TTATAAAATGCCAAATGGTGATATTCTTGACTATGCACCATTAGCGGCAAAGGACTGGGAAGGTGTCGAGCCAATTTATG
AAACAATGCCGGGTTGGAAAGAAAATACCTTTGGCGTAACGGATGTCAACCAATTACCAGAAGCCTGCCGTAATTATGTC
AAACGTATTGAAGAAGTCACGGGTGTGCCAGTAGACATCCTTTCAACCGGTCCAGACCGTGTTGAGACCATGATCTTACG
TGATCCTTTTGCTGCCTAA

Upstream 100 bases:

>100_bases
CAAATAAAAACGTAAATTACATTGAGATTAATGTAAGAAAATAGTAAAGTAATCCGTTGTTCATTTTATTTATATACAAA
GCTTAAAGGCGGAAAATACT

Downstream 100 bases:

>100_bases
TTAGCACCCTATTATCATATCAAAATGGAAAGCGAAATCAGCTTTCCATTTTCACCTCCCCCTCCAAATCAATAGATTTA
AACTATTAATTAAATAGATT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRENVTCLIGNGVVLSPAAL
MQEMGELESRGINVRERLLISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVGDLFNREAFAE
KLKTILDYYNFQLVNYYKAEAVDFQKTLDDVMAVADIITGMVADVTTMLDKARKNGDNILFEGAQGTMLDIDHGTYPFVT
SSNTTAGGVATGAGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDEVGSEIARKGNEFGAVTGRPRRCGWFDAVAIRRAI
QLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGDILDYAPLAAKDWEGVEPIYETMPGWKENTFGVTDVNQLPEACRNYV
KRIEEVTGVPVDILSTGPDRVETMILRDPFAA

Sequences:

>Translated_432_residues
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRENVTCLIGNGVVLSPAAL
MQEMGELESRGINVRERLLISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVGDLFNREAFAE
KLKTILDYYNFQLVNYYKAEAVDFQKTLDDVMAVADIITGMVADVTTMLDKARKNGDNILFEGAQGTMLDIDHGTYPFVT
SSNTTAGGVATGAGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDEVGSEIARKGNEFGAVTGRPRRCGWFDAVAIRRAI
QLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGDILDYAPLAAKDWEGVEPIYETMPGWKENTFGVTDVNQLPEACRNYV
KRIEEVTGVPVDILSTGPDRVETMILRDPFAA
>Mature_431_residues
GKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRENVTCLIGNGVVLSPAALM
QEMGELESRGINVRERLLISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVGDLFNREAFAEK
LKTILDYYNFQLVNYYKAEAVDFQKTLDDVMAVADIITGMVADVTTMLDKARKNGDNILFEGAQGTMLDIDHGTYPFVTS
SNTTAGGVATGAGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDEVGSEIARKGNEFGAVTGRPRRCGWFDAVAIRRAIQ
LNSISGFCMTKLDVLDGFDEVKICVAYKMPNGDILDYAPLAAKDWEGVEPIYETMPGWKENTFGVTDVNQLPEACRNYVK
RIEEVTGVPVDILSTGPDRVETMILRDPFAA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=42.093023255814, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=41.6666666666667, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=40.7035175879397, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=76.8518518518518, Blast_Score=705, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=42.4942263279446, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=42.4942263279446, Blast_Score=337, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=41.6091954022989, Blast_Score=329, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.4170616113744, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47158; Mature: 47026

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCHHEEHHHHHHH
LRENVTCLIGNGVVLSPAALMQEMGELESRGINVRERLLISEACPLILPYHVAMDHAREA
HHCCCEEEEECCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ALGKNKIGTTGRGIGPAYEDKVARRGLRVGDLFNREAFAEKLKTILDYYNFQLVNYYKAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHH
AVDFQKTLDDVMAVADIITGMVADVTTMLDKARKNGDNILFEGAQGTMLDIDHGTYPFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGAGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDEVGSEIARKGNEFGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAIRRAIQLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGDILDYAP
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEECCCC
LAAKDWEGVEPIYETMPGWKENTFGVTDVNQLPEACRNYVKRIEEVTGVPVDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
VETMILRDPFAA
HHEEEECCCCCC
>Mature Secondary Structure 
GKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCHHEEHHHHHHH
LRENVTCLIGNGVVLSPAALMQEMGELESRGINVRERLLISEACPLILPYHVAMDHAREA
HHCCCEEEEECCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ALGKNKIGTTGRGIGPAYEDKVARRGLRVGDLFNREAFAEKLKTILDYYNFQLVNYYKAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHH
AVDFQKTLDDVMAVADIITGMVADVTTMLDKARKNGDNILFEGAQGTMLDIDHGTYPFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGAGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDEVGSEIARKGNEFGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAIRRAIQLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGDILDYAP
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEECCCC
LAAKDWEGVEPIYETMPGWKENTFGVTDVNQLPEACRNYVKRIEEVTGVPVDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
VETMILRDPFAA
HHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA