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The map label for this gene is mpl [H]

Identifier: 15602794

GI number: 15602794

Start: 1096528

End: 1097901

Strand: Reverse

Name: mpl [H]

Synonym: PM0929

Alternate gene names: 15602794

Gene position: 1097901-1096528 (Counterclockwise)

Preceding gene: 15602799

Following gene: 15602793

Centisome position: 48.63

GC content: 42.43

Gene sequence:

>1374_bases
ATGACAGTAAAGCATATCCACATCTTAGGTATTTGTGGCACGTTTATGGGCGGTGTCGCCATGATTGCGAAACAACTTGG
TTATAAAGTGACAGGCTCCGATACCCATGTTTATCCGCCAATGAGCACATTTTTGATGGAAAATGGAATTGAGATTATTC
CACATTTTGATCCGACACAATTACAGCCAGCGCCGGATATGGTGATCATTGGTAACGCAATGAAACGCGGTAATCCTTGC
GTAGAATATGTATTGGAACATCATTTACCCTATACCTCAGGTCCACAATGGTTACATGATCATTTGTTGCGTGATCGTTG
GGTATTGGCTGTATCGGGTACCCATGGAAAAACTACAACAACTGGCATGTTAAGTTGGATTTTAGATAAGTGCGGTCTTA
AACCTGGTTTTTTAATTGGGGGGATCTCGGGCAATTTTGGTATATCCGCTCGACTTGGGGAAAGTGATTATTTCGTGATT
GAAGCCGATGAGTATGATACGGCATTTTTTGATAAACGTTCTAAATTTGTCCATTATAATCCTAAGACCTTGATTATTAA
TAATATCGGGTTTGATCATGCGGATATTTTTGATGATCTGAAAGCCATTCAGCGCCAATTTCATCATATGATTCGTACGA
TGCCAGCCAGTGGACGCATTCTTTCTGTGGCGAATGAGCCGAATGTGAAAGCAACCTTAGACATGGGTTGTTGGTCGCAA
CAACAGTTTTTAGGTCAGGATCAAGACTGGTATGCTGAACGGATCAGCCATGATTGCACGCAATTTGGTGTTTTCTACCA
AGGCGAGAAAATGGCAGAGGTGTATTGGAATGTCGTCGGTCAACATAATATGCACAATGCATTAATGGCGATTGCTGCGG
CACATCATGTCGGGATTTCAATTGCTGAGGCATGTCAGGCACTTGCCACGTTTATTAGCCCGAAACGCCGTTTAGAAGTG
AAAGGGCAAGTTAATGGCATTACGGTTTATGATGATTTTGCTCATCATCCGACAGAAATTCTGGCAACATTGACCGCACT
TCGTGACAAAGTTGGAGGGGGCGTGCGTATTTTGGCAGTACTTGAGCCACGTTCAAATACTATGAAAATGGGCGTGCATA
AAGACGAGATTGCGCCTGCTTTAGGTAGAGCGGATAACGTATTCTTATTTCAGCCTGAAAATATTGGTTGGGATGTGGTG
GATATTGTGAGTCAGTGTGTCCAGCCAGCAGTTTGGAATATGAATCTTGATAAGTTGGTGGAGATGATTGCTGATGAAGC
GAAACCAACAGATCATATTTTGGTGATGTCAAATGGCAGTTTCGGGGGGATCCATCAAAAACTGTTAGACAAGTTAGCTT
TAAAAACAGAATAA

Upstream 100 bases:

>100_bases
TTTATTATAGCAACCTTAGGTTTATCATAGAAAAGTCGAATGTTAACAATGTGATCTTCGACACATTTTTTATGACAATA
GATGACATAGAGATAAAAAA

Downstream 100 bases:

>100_bases
AGTACTTGCTTAAATGAAAGTATAAAAGGCAAACGTGAGTTTGCCTTTATTTGTTTGAGAGAGACTTTCATGCTTGTGAT
TTGTGAGTCTGTTTTTTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTVKHIHILGICGTFMGGVAMIAKQLGYKVTGSDTHVYPPMSTFLMENGIEIIPHFDPTQLQPAPDMVIIGNAMKRGNPC
VEYVLEHHLPYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLSWILDKCGLKPGFLIGGISGNFGISARLGESDYFVI
EADEYDTAFFDKRSKFVHYNPKTLIINNIGFDHADIFDDLKAIQRQFHHMIRTMPASGRILSVANEPNVKATLDMGCWSQ
QQFLGQDQDWYAERISHDCTQFGVFYQGEKMAEVYWNVVGQHNMHNALMAIAAAHHVGISIAEACQALATFISPKRRLEV
KGQVNGITVYDDFAHHPTEILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADNVFLFQPENIGWDVV
DIVSQCVQPAVWNMNLDKLVEMIADEAKPTDHILVMSNGSFGGIHQKLLDKLALKTE

Sequences:

>Translated_457_residues
MTVKHIHILGICGTFMGGVAMIAKQLGYKVTGSDTHVYPPMSTFLMENGIEIIPHFDPTQLQPAPDMVIIGNAMKRGNPC
VEYVLEHHLPYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLSWILDKCGLKPGFLIGGISGNFGISARLGESDYFVI
EADEYDTAFFDKRSKFVHYNPKTLIINNIGFDHADIFDDLKAIQRQFHHMIRTMPASGRILSVANEPNVKATLDMGCWSQ
QQFLGQDQDWYAERISHDCTQFGVFYQGEKMAEVYWNVVGQHNMHNALMAIAAAHHVGISIAEACQALATFISPKRRLEV
KGQVNGITVYDDFAHHPTEILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADNVFLFQPENIGWDVV
DIVSQCVQPAVWNMNLDKLVEMIADEAKPTDHILVMSNGSFGGIHQKLLDKLALKTE
>Mature_456_residues
TVKHIHILGICGTFMGGVAMIAKQLGYKVTGSDTHVYPPMSTFLMENGIEIIPHFDPTQLQPAPDMVIIGNAMKRGNPCV
EYVLEHHLPYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLSWILDKCGLKPGFLIGGISGNFGISARLGESDYFVIE
ADEYDTAFFDKRSKFVHYNPKTLIINNIGFDHADIFDDLKAIQRQFHHMIRTMPASGRILSVANEPNVKATLDMGCWSQQ
QFLGQDQDWYAERISHDCTQFGVFYQGEKMAEVYWNVVGQHNMHNALMAIAAAHHVGISIAEACQALATFISPKRRLEVK
GQVNGITVYDDFAHHPTEILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADNVFLFQPENIGWDVVD
IVSQCVQPAVWNMNLDKLVEMIADEAKPTDHILVMSNGSFGGIHQKLLDKLALKTE

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=452, Percent_Identity=66.3716814159292, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=484, Percent_Identity=30.1652892561983, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50813; Mature: 50682

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKHIHILGICGTFMGGVAMIAKQLGYKVTGSDTHVYPPMSTFLMENGIEIIPHFDPTQ
CCEEEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCHHHHHHHCCCEEECCCCCCC
LQPAPDMVIIGNAMKRGNPCVEYVLEHHLPYTSGPQWLHDHLLRDRWVLAVSGTHGKTTT
CCCCCCEEEEECCHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHH
TGMLSWILDKCGLKPGFLIGGISGNFGISARLGESDYFVIEADEYDTAFFDKRSKFVHYN
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCCCCHHHHCCCCEEEEC
PKTLIINNIGFDHADIFDDLKAIQRQFHHMIRTMPASGRILSVANEPNVKATLDMGCWSQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCH
QQFLGQDQDWYAERISHDCTQFGVFYQGEKMAEVYWNVVGQHNMHNALMAIAAAHHVGIS
HHHCCCCHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCH
IAEACQALATFISPKRRLEVKGQVNGITVYDDFAHHPTEILATLTALRDKVGGGVRILAV
HHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
LEPRSNTMKMGVHKDEIAPALGRADNVFLFQPENIGWDVVDIVSQCVQPAVWNMNLDKLV
ECCCCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
EMIADEAKPTDHILVMSNGSFGGIHQKLLDKLALKTE
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKHIHILGICGTFMGGVAMIAKQLGYKVTGSDTHVYPPMSTFLMENGIEIIPHFDPTQ
CEEEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCHHHHHHHCCCEEECCCCCCC
LQPAPDMVIIGNAMKRGNPCVEYVLEHHLPYTSGPQWLHDHLLRDRWVLAVSGTHGKTTT
CCCCCCEEEEECCHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHH
TGMLSWILDKCGLKPGFLIGGISGNFGISARLGESDYFVIEADEYDTAFFDKRSKFVHYN
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCCCCHHHHCCCCEEEEC
PKTLIINNIGFDHADIFDDLKAIQRQFHHMIRTMPASGRILSVANEPNVKATLDMGCWSQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCH
QQFLGQDQDWYAERISHDCTQFGVFYQGEKMAEVYWNVVGQHNMHNALMAIAAAHHVGIS
HHHCCCCHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCH
IAEACQALATFISPKRRLEVKGQVNGITVYDDFAHHPTEILATLTALRDKVGGGVRILAV
HHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
LEPRSNTMKMGVHKDEIAPALGRADNVFLFQPENIGWDVVDIVSQCVQPAVWNMNLDKLV
ECCCCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
EMIADEAKPTDHILVMSNGSFGGIHQKLLDKLALKTE
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]