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The map label for this gene is murI

Identifier: 15602782

GI number: 15602782

Start: 1082228

End: 1083037

Strand: Reverse

Name: murI

Synonym: PM0917

Alternate gene names: 15602782

Gene position: 1083037-1082228 (Counterclockwise)

Preceding gene: 15602783

Following gene: 15602781

Centisome position: 47.98

GC content: 37.16

Gene sequence:

>810_bases
ATGAATAGCCCTTATCAACCTACTATCCTATTTTTTGATTCCGGTATGGGCGGCTTTAGTGTTTATAAAGAAACAAAGCA
ACTTTTACCCGATTGTCACTATCTTTATTGTTTCGATAATGCGTTTTTTCCTTATTCGGAAAAAAGTGAAGAGGAAATTA
TTCAGCGTACGCTCAAAATTTGTCAAAAAATTGACCGCACTTTCCCTTTAGACTTAATTGTAATTGCGTGTAATACGGCA
AGCACGGTGGTATTACCCGCACTAAGAGCGCATTTTGCGATTCCTGTCGTGGGAACTGTGCCTGCAATTAAACCCGCAGC
AGAATGTTCAGAAACAAAACATATTGGCTTATTAGCTACAAAAGGCACCGTGAAAAGAGCCTATGTCGCAGATTTAATTG
CACAGTATGCTCGCGATTGCACAGTTGAGAAATTGGGCAGTACAAAATTAGTCGAAATTGCTGAACAAAAGTTACATGGC
AAAGCGGTTGATTTGCATGCTTTAAAGCAAGAGCTAGCGCCTTGGCAGTCAATTCAGAATCTTGATACAGTCGTATTAGG
TTGTACGCACTTTCCTTTAATTAAAGAAGAAATTAAATGGTGTTTACCACAAGTGCGTTTTTTTGTTGATTCTGGTAAAG
CGATTGCTTTACGGGTTAAAACGTTATTGTCTAAGATAGACATTCAATCTAAAACTAACGAGAAAAATCTTATTTTTTGT
ACTCAATTTTTTGAGGATGAAACACAGTTTCAAAAGGTTATCCATTTTTGGGGATTTGAACAACTCATCAATTTGAACAT
GAAAGCCTGA

Upstream 100 bases:

>100_bases
CCGCACTTTATGCCCGCCGTGCCTTAATGAGTATTGGCGCTGAGAATATGATGGTTGCTGAATTATTTCTAACACCACAA
ATTTTTACGAGAGTAGTCAA

Downstream 100 bases:

>100_bases
AAAGAATACGATTTTTTTATAAATTACGAGCATAACGTTTAAAAAAATAGCGCAAAATAAAAAATTTAATTTTTTTTGTA
AAAAGGGGTTGCAAAGGGTT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNSPYQPTILFFDSGMGGFSVYKETKQLLPDCHYLYCFDNAFFPYSEKSEEEIIQRTLKICQKIDRTFPLDLIVIACNTA
STVVLPALRAHFAIPVVGTVPAIKPAAECSETKHIGLLATKGTVKRAYVADLIAQYARDCTVEKLGSTKLVEIAEQKLHG
KAVDLHALKQELAPWQSIQNLDTVVLGCTHFPLIKEEIKWCLPQVRFFVDSGKAIALRVKTLLSKIDIQSKTNEKNLIFC
TQFFEDETQFQKVIHFWGFEQLINLNMKA

Sequences:

>Translated_269_residues
MNSPYQPTILFFDSGMGGFSVYKETKQLLPDCHYLYCFDNAFFPYSEKSEEEIIQRTLKICQKIDRTFPLDLIVIACNTA
STVVLPALRAHFAIPVVGTVPAIKPAAECSETKHIGLLATKGTVKRAYVADLIAQYARDCTVEKLGSTKLVEIAEQKLHG
KAVDLHALKQELAPWQSIQNLDTVVLGCTHFPLIKEEIKWCLPQVRFFVDSGKAIALRVKTLLSKIDIQSKTNEKNLIFC
TQFFEDETQFQKVIHFWGFEQLINLNMKA
>Mature_269_residues
MNSPYQPTILFFDSGMGGFSVYKETKQLLPDCHYLYCFDNAFFPYSEKSEEEIIQRTLKICQKIDRTFPLDLIVIACNTA
STVVLPALRAHFAIPVVGTVPAIKPAAECSETKHIGLLATKGTVKRAYVADLIAQYARDCTVEKLGSTKLVEIAEQKLHG
KAVDLHALKQELAPWQSIQNLDTVVLGCTHFPLIKEEIKWCLPQVRFFVDSGKAIALRVKTLLSKIDIQSKTNEKNLIFC
TQFFEDETQFQKVIHFWGFEQLINLNMKA

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=261, Percent_Identity=52.1072796934866, Blast_Score=264, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_PASMU (P57887)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245854.1
- ProteinModelPortal:   P57887
- SMR:   P57887
- GeneID:   1244264
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0917
- NMPDR:   fig|272843.1.peg.917
- HOGENOM:   HBG645102
- OMA:   ARTNAMI
- ProtClustDB:   PRK00865
- BioCyc:   PMUL272843:PM0917-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 30435; Mature: 30435

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPYQPTILFFDSGMGGFSVYKETKQLLPDCHYLYCFDNAFFPYSEKSEEEIIQRTLKI
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
CQKIDRTFPLDLIVIACNTASTVVLPALRAHFAIPVVGTVPAIKPAAECSETKHIGLLAT
HHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEE
KGTVKRAYVADLIAQYARDCTVEKLGSTKLVEIAEQKLHGKAVDLHALKQELAPWQSIQN
CCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHC
LDTVVLGCTHFPLIKEEIKWCLPQVRFFVDSGKAIALRVKTLLSKIDIQSKTNEKNLIFC
CHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEE
TQFFEDETQFQKVIHFWGFEQLINLNMKA
EHHHCCHHHHHHHHHHHCHHHHHCCCCCC
>Mature Secondary Structure
MNSPYQPTILFFDSGMGGFSVYKETKQLLPDCHYLYCFDNAFFPYSEKSEEEIIQRTLKI
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
CQKIDRTFPLDLIVIACNTASTVVLPALRAHFAIPVVGTVPAIKPAAECSETKHIGLLAT
HHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEE
KGTVKRAYVADLIAQYARDCTVEKLGSTKLVEIAEQKLHGKAVDLHALKQELAPWQSIQN
CCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHC
LDTVVLGCTHFPLIKEEIKWCLPQVRFFVDSGKAIALRVKTLLSKIDIQSKTNEKNLIFC
CHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEE
TQFFEDETQFQKVIHFWGFEQLINLNMKA
EHHHCCHHHHHHHHHHHCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100