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The map label for this gene is amiB [H]

Identifier: 15602768

GI number: 15602768

Start: 1060625

End: 1061854

Strand: Direct

Name: amiB [H]

Synonym: PM0903

Alternate gene names: 15602768

Gene position: 1060625-1061854 (Clockwise)

Preceding gene: 15602767

Following gene: 15602769

Centisome position: 46.98

GC content: 41.38

Gene sequence:

>1230_bases
ATGAAAAAATATCGTTTTATTTCACTTTTCTTTGCAACAATACTTTTTATTTCAACACCATTATTCGCAAATAATGTGTG
GACCATTGCTATTGACCCTGGACATGGTGGCACCGATCCGGGTGCGATTAGTCGTCAATTAAAAATGTATGAAAAAAATG
TGACACTCTCTATTGCGAGAGAGCTCAAGATGCTCCTTGATAAAGATCCACATTTCAAAGGGGTACTCACACGTACTGGT
GATTATTTTATTTCGGTCCCGAAACGTTCTGAAATTGCCCGTAAACATAAAGCCAACCTTTTGATCTCGATCCATGCTGA
CTCAAATTTATCGCCTTTATTACGTGGCGCATCCGTTTGGGTACTTTCGAATCGTCGCGCAAACAGTGAAATGGGACAAT
GGTTAGAGGATCACGAAAAACGTTCCGAACTGCTCGGCGGCGCCGGCACGGTTTTGGCCTCCCACAATGAAAAGTATTTA
GATCAAACCGTCTTAGATCTCCAATTTGGTCACAGTCAACGTGTTGGTTATGAACTCGGTTCTATCGTCTTAAAACGCTT
TGCCAAAATTACGACCTTAAATCGAAGTACGCCACAGCATGCCAGCCTTGGCGTGTTACGCTCGCCGGATATTCCTTCTA
TCTTAGTTGAAACAGGCTTTCTCTCGAACGTCGATGAAGAGAAAAAACTCAGCACTTTGGCTTATCGTAAAAAAGTGGCT
TATATGATTTACGAAAGCCTAGTGGAATACCGTAAAAAATCAGTTGCGAATGAGCCAAAAACCAAATCCCCAACAACAGT
CGAAAGCAGTGCAACACAGACGACGCTCACAGACAGTGGACTACGTCATAAAGTCAAAGCAGGGGAAAATCTTACCGGCG
TTGCACGCAAATATGGGGTGAAAGTGAATGATATCTTAGCACTCAATAAATTAAAACGACGCGAATTATGGATTGGTGAA
ACCTTAAAAATTCCTGCTAGCGGAAAAGCCAGTGAAAAAACCACACCAGCTACAACCAGTAAAGTGGCAGAAACAAAAAC
GAAAGTCGTAAGCAACACAACATCGGAATCAAAAGAGAAACTGCCGAAATACCATACCGTGAAAAAAGGGCAAACACTCT
ACGCGATTGCCAGAGAGTACCATATTCCACCAAATCAGCTATTAAAATTAAATCCCCATTTAAAAGATGGCAAAGTGTTA
ACAGGACAAAAAATTAAATTGAGAGATTGA

Upstream 100 bases:

>100_bases
CAATATGTTGATCATGCGAGAAATCTTGAATTAATCGCGAATTCTCCACAAGGTGAACAAATTATTGCACAACTTAGAAA
AATCGAAATCACATAGCACA

Downstream 100 bases:

>100_bases
TCAGTATGGCGATTAAAGTTCTTTCTCCTCAGCTTGCTAACCAGATCGCCGCGGGCGAAGTCGTTGAACGCCCAGCTTCT
GTGGTGAAAGAGTTGGTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKKYRFISLFFATILFISTPLFANNVWTIAIDPGHGGTDPGAISRQLKMYEKNVTLSIARELKMLLDKDPHFKGVLTRTG
DYFISVPKRSEIARKHKANLLISIHADSNLSPLLRGASVWVLSNRRANSEMGQWLEDHEKRSELLGGAGTVLASHNEKYL
DQTVLDLQFGHSQRVGYELGSIVLKRFAKITTLNRSTPQHASLGVLRSPDIPSILVETGFLSNVDEEKKLSTLAYRKKVA
YMIYESLVEYRKKSVANEPKTKSPTTVESSATQTTLTDSGLRHKVKAGENLTGVARKYGVKVNDILALNKLKRRELWIGE
TLKIPASGKASEKTTPATTSKVAETKTKVVSNTTSESKEKLPKYHTVKKGQTLYAIAREYHIPPNQLLKLNPHLKDGKVL
TGQKIKLRD

Sequences:

>Translated_409_residues
MKKYRFISLFFATILFISTPLFANNVWTIAIDPGHGGTDPGAISRQLKMYEKNVTLSIARELKMLLDKDPHFKGVLTRTG
DYFISVPKRSEIARKHKANLLISIHADSNLSPLLRGASVWVLSNRRANSEMGQWLEDHEKRSELLGGAGTVLASHNEKYL
DQTVLDLQFGHSQRVGYELGSIVLKRFAKITTLNRSTPQHASLGVLRSPDIPSILVETGFLSNVDEEKKLSTLAYRKKVA
YMIYESLVEYRKKSVANEPKTKSPTTVESSATQTTLTDSGLRHKVKAGENLTGVARKYGVKVNDILALNKLKRRELWIGE
TLKIPASGKASEKTTPATTSKVAETKTKVVSNTTSESKEKLPKYHTVKKGQTLYAIAREYHIPPNQLLKLNPHLKDGKVL
TGQKIKLRD
>Mature_409_residues
MKKYRFISLFFATILFISTPLFANNVWTIAIDPGHGGTDPGAISRQLKMYEKNVTLSIARELKMLLDKDPHFKGVLTRTG
DYFISVPKRSEIARKHKANLLISIHADSNLSPLLRGASVWVLSNRRANSEMGQWLEDHEKRSELLGGAGTVLASHNEKYL
DQTVLDLQFGHSQRVGYELGSIVLKRFAKITTLNRSTPQHASLGVLRSPDIPSILVETGFLSNVDEEKKLSTLAYRKKVA
YMIYESLVEYRKKSVANEPKTKSPTTVESSATQTTLTDSGLRHKVKAGENLTGVARKYGVKVNDILALNKLKRRELWIGE
TLKIPASGKASEKTTPATTSKVAETKTKVVSNTTSESKEKLPKYHTVKKGQTLYAIAREYHIPPNQLLKLNPHLKDGKVL
TGQKIKLRD

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=250, Percent_Identity=57.2, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=40.0900900900901, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082163, Length=223, Percent_Identity=38.5650224215247, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45673; Mature: 45673

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYRFISLFFATILFISTPLFANNVWTIAIDPGHGGTDPGAISRQLKMYEKNVTLSIAR
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
ELKMLLDKDPHFKGVLTRTGDYFISVPKRSEIARKHKANLLISIHADSNLSPLLRGASVW
HHHHHHCCCCCCCEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHCCCEEE
VLSNRRANSEMGQWLEDHEKRSELLGGAGTVLASHNEKYLDQTVLDLQFGHSQRVGYELG
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHH
SIVLKRFAKITTLNRSTPQHASLGVLRSPDIPSILVETGFLSNVDEEKKLSTLAYRKKVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
YMIYESLVEYRKKSVANEPKTKSPTTVESSATQTTLTDSGLRHKVKAGENLTGVARKYGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCC
KVNDILALNKLKRRELWIGETLKIPASGKASEKTTPATTSKVAETKTKVVSNTTSESKEK
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
LPKYHTVKKGQTLYAIAREYHIPPNQLLKLNPHLKDGKVLTGQKIKLRD
CCCCEECCCCCEEEEEHHHCCCCHHHEEEECCCCCCCEEECCCEEEECC
>Mature Secondary Structure
MKKYRFISLFFATILFISTPLFANNVWTIAIDPGHGGTDPGAISRQLKMYEKNVTLSIAR
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
ELKMLLDKDPHFKGVLTRTGDYFISVPKRSEIARKHKANLLISIHADSNLSPLLRGASVW
HHHHHHCCCCCCCEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHCCCEEE
VLSNRRANSEMGQWLEDHEKRSELLGGAGTVLASHNEKYLDQTVLDLQFGHSQRVGYELG
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHH
SIVLKRFAKITTLNRSTPQHASLGVLRSPDIPSILVETGFLSNVDEEKKLSTLAYRKKVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
YMIYESLVEYRKKSVANEPKTKSPTTVESSATQTTLTDSGLRHKVKAGENLTGVARKYGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCC
KVNDILALNKLKRRELWIGETLKIPASGKASEKTTPATTSKVAETKTKVVSNTTSESKEK
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
LPKYHTVKKGQTLYAIAREYHIPPNQLLKLNPHLKDGKVLTGQKIKLRD
CCCCEECCCCCEEEEEHHHCCCCHHHEEEECCCCCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]