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The map label for this gene is ptsI [H]

Identifier: 15602762

GI number: 15602762

Start: 1055574

End: 1057301

Strand: Reverse

Name: ptsI [H]

Synonym: PM0897

Alternate gene names: 15602762

Gene position: 1057301-1055574 (Counterclockwise)

Preceding gene: 15602763

Following gene: 15602761

Centisome position: 46.84

GC content: 38.89

Gene sequence:

>1728_bases
ATGATTTCAGGAATTCCAGCTTCACCAGGTATCGTTTTTGGTAAAGCCTTAGTGCTTAAAGAAGAAAAAATTGTACTGGA
TATGCAAAAAATCAGTGAATCTCAGATTGATTCGGAAATTGCACGTTTTTATCAAGGACGAAATGCGGCAGTAGAACAGC
TTAGTGCCATTCGCGATCGGGCGGCGAAGACCTTAGGTGAAGAAAAAGCAGCAATTTTTGAAGGTCATTTGATGATCTTG
GAAGATGAGGAGTTGGAAGAAGAAATTATCGATTATTTGCGTACAAATAAAGTGAATGCGGGTGTAGCTGCAAGTAAAAT
TATTGATCAGCAAGTCACGATGTTATCTGAAATTGATGATGAATATTTAAGAGAACGTGCCGGCGATATTCGAGATATTG
GTAATCGTTTAATTAAAAATATTTTAGGCATGCATATTGTTGATTTAGGTGATATCAACGAAGAAGCGATTTTGGTGGCT
TATGATTTAACGCCCTCTGAGACAGCACAGCTTAATTTAGACAAAGTCTTAGGTTTTATTACCGACATCGGTGGACGTAC
TTCACACACATCTATTATGGCTCGTTCTTTAGAGCTACCGGCGATTGTTGGAACAAATCATGTCACTTCACAAGTGAATA
CTGGCGATTACCTGATTTTAGATGCGGTGAATAACGTCGTTTACGTTAATCCATCACAAGATGAAATAACGCGCTTAAAA
GGCTTACAACAAAAACTCTTAGAAGAAAAAGCAGAATTAGCCAAATTAAAAGATTTACCCGCGTTAACCTTAGATGGTCA
TCGTGTCGACGTGGTTGCCAATATTGGTACGATTCGTGATGTAGATGGGGCAGATCGTAATGGGGCAGAAGGCGTAGGCT
TGTATCGAACCGAATTCTTATTTATGGATCGTGAGCAATTACCGACTGAAGAAGAACAATTTGTTGCTTACAAAGACGTT
GTTGAAGCGATGAATGGTCGCCTCGTGATTTTACGTACCATGGATATTGGTGGTGATAAAGAGCTTCCTTATTTAAATTT
ACCAAAAGAAATGAACCCCTTCTTAGGGTGGCGTGCAATTCGTATCGCATTAGACCGTCGTGAAATTTTACATGCACAAT
TGAGAGCCGTGTTACGCGCATCTGCTTTTGGTAAGTTAGCGGTTATGTTCCCAATGATTATTTCGGTGGAAGAGATTCGC
GAATTGAAGTCAGTCATTGAAACCTTAAAACAAGAATTGCGTAATGAAGGCAAGGCATTTGATGAAAGTATTCAAATCGG
TGTCATGGTAGAAACACCGTCAGCAGCCGTGAATGCCAAATTCTTAGCCAAAGAAGTGGATTTCTTTAGTATTGGAACAA
ACGATTTAACACAATACACATTAGCAGTTGACCGTGGTAATGAATTAATTTCTCACTTGTATAATCCAATGTCACCTTCT
GTCTTAAGTTTAATTAAACAGGTGATTGATGCTTCGCATGCAGAAGGAAAATGGACTGGCATGTGTGGTGAGCTTGCGGG
TGATGAAAAAGCCGCGGTATTACTTTTAGGTATGGGCTTGGATGAATTTAGTATGAGTGCTATCTCTGTACCACGTATTA
AGAAATTGATCCGTAATGTGAATTATGAAGATACAAAAGCCTTAGCAATGAAGGCATTACAGCAACCAACAGCCGTAGAA
ATTGAACAATTAGTTGAAGATTTTTTAGCTGAAAAAGCATTAAATTAG

Upstream 100 bases:

>100_bases
CCAACCTTAGAATAATTGATTGTTAGCCATAATGTGAGTCCACATTATGGCGTTTATTTTTTTCCATCATCACATTTTAG
TAAAGTTCAGAAGGTTACAT

Downstream 100 bases:

>100_bases
ATACAAGCTGAAAATTTTACGTTAAAATATGACCACATTTACTTAGTAGGAGACTAAAATGGGTTTATTTGACAAATTAT
TTGGTTCAAAAGACAAAAAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGIPASPGIVFGKALVLKEEKIVLDMQKISESQIDSEIARFYQGRNAAVEQLSAIRDRAAKTLGEEKAAIFEGHLMIL
EDEELEEEIIDYLRTNKVNAGVAASKIIDQQVTMLSEIDDEYLRERAGDIRDIGNRLIKNILGMHIVDLGDINEEAILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNHVTSQVNTGDYLILDAVNNVVYVNPSQDEITRLK
GLQQKLLEEKAELAKLKDLPALTLDGHRVDVVANIGTIRDVDGADRNGAEGVGLYRTEFLFMDREQLPTEEEQFVAYKDV
VEAMNGRLVILRTMDIGGDKELPYLNLPKEMNPFLGWRAIRIALDRREILHAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKSVIETLKQELRNEGKAFDESIQIGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLSLIKQVIDASHAEGKWTGMCGELAGDEKAAVLLLGMGLDEFSMSAISVPRIKKLIRNVNYEDTKALAMKALQQPTAVE
IEQLVEDFLAEKALN

Sequences:

>Translated_575_residues
MISGIPASPGIVFGKALVLKEEKIVLDMQKISESQIDSEIARFYQGRNAAVEQLSAIRDRAAKTLGEEKAAIFEGHLMIL
EDEELEEEIIDYLRTNKVNAGVAASKIIDQQVTMLSEIDDEYLRERAGDIRDIGNRLIKNILGMHIVDLGDINEEAILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNHVTSQVNTGDYLILDAVNNVVYVNPSQDEITRLK
GLQQKLLEEKAELAKLKDLPALTLDGHRVDVVANIGTIRDVDGADRNGAEGVGLYRTEFLFMDREQLPTEEEQFVAYKDV
VEAMNGRLVILRTMDIGGDKELPYLNLPKEMNPFLGWRAIRIALDRREILHAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKSVIETLKQELRNEGKAFDESIQIGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLSLIKQVIDASHAEGKWTGMCGELAGDEKAAVLLLGMGLDEFSMSAISVPRIKKLIRNVNYEDTKALAMKALQQPTAVE
IEQLVEDFLAEKALN
>Mature_575_residues
MISGIPASPGIVFGKALVLKEEKIVLDMQKISESQIDSEIARFYQGRNAAVEQLSAIRDRAAKTLGEEKAAIFEGHLMIL
EDEELEEEIIDYLRTNKVNAGVAASKIIDQQVTMLSEIDDEYLRERAGDIRDIGNRLIKNILGMHIVDLGDINEEAILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNHVTSQVNTGDYLILDAVNNVVYVNPSQDEITRLK
GLQQKLLEEKAELAKLKDLPALTLDGHRVDVVANIGTIRDVDGADRNGAEGVGLYRTEFLFMDREQLPTEEEQFVAYKDV
VEAMNGRLVILRTMDIGGDKELPYLNLPKEMNPFLGWRAIRIALDRREILHAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKSVIETLKQELRNEGKAFDESIQIGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLSLIKQVIDASHAEGKWTGMCGELAGDEKAAVLLLGMGLDEFSMSAISVPRIKKLIRNVNYEDTKALAMKALQQPTAVE
IEQLVEDFLAEKALN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=72.2996515679443, Blast_Score=841, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=515, Percent_Identity=40.9708737864078, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=38.7351778656126, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1789193, Length=585, Percent_Identity=33.1623931623932, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787994, Length=453, Percent_Identity=28.6975717439294, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI226510935, Length=216, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63687; Mature: 63687

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGIPASPGIVFGKALVLKEEKIVLDMQKISESQIDSEIARFYQGRNAAVEQLSAIRDR
CCCCCCCCCCCEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAKTLGEEKAAIFEGHLMILEDEELEEEIIDYLRTNKVNAGVAASKIIDQQVTMLSEIDD
HHHHHCHHHHHEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EYLRERAGDIRDIGNRLIKNILGMHIVDLGDINEEAILVAYDLTPSETAQLNLDKVLGFI
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNHVTSQVNTGDYLILDAVNNVVYVNPSQDEITRLK
HHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHH
GLQQKLLEEKAELAKLKDLPALTLDGHRVDVVANIGTIRDVDGADRNGAEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCEECCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFVAYKDVVEAMNGRLVILRTMDIGGDKELPYLNLPKEMNPFLGWRAI
EECHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCHHCCHHHCHHHH
RIALDRREILHAQLRAVLRASAFGKLAVMFPMIISVEEIRELKSVIETLKQELRNEGKAF
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DESIQIGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCHHEEEEEECCHHHHHHHHCCCCCHH
VLSLIKQVIDASHAEGKWTGMCGELAGDEKAAVLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NYEDTKALAMKALQQPTAVEIEQLVEDFLAEKALN
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISGIPASPGIVFGKALVLKEEKIVLDMQKISESQIDSEIARFYQGRNAAVEQLSAIRDR
CCCCCCCCCCCEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAKTLGEEKAAIFEGHLMILEDEELEEEIIDYLRTNKVNAGVAASKIIDQQVTMLSEIDD
HHHHHCHHHHHEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EYLRERAGDIRDIGNRLIKNILGMHIVDLGDINEEAILVAYDLTPSETAQLNLDKVLGFI
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNHVTSQVNTGDYLILDAVNNVVYVNPSQDEITRLK
HHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHH
GLQQKLLEEKAELAKLKDLPALTLDGHRVDVVANIGTIRDVDGADRNGAEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCEECCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFVAYKDVVEAMNGRLVILRTMDIGGDKELPYLNLPKEMNPFLGWRAI
EECHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCHHCCHHHCHHHH
RIALDRREILHAQLRAVLRASAFGKLAVMFPMIISVEEIRELKSVIETLKQELRNEGKAF
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DESIQIGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCHHEEEEEECCHHHHHHHHCCCCCHH
VLSLIKQVIDASHAEGKWTGMCGELAGDEKAAVLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NYEDTKALAMKALQQPTAVEIEQLVEDFLAEKALN
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]