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The map label for this gene is aceF [H]

Identifier: 15602759

GI number: 15602759

Start: 1049678

End: 1051576

Strand: Reverse

Name: aceF [H]

Synonym: PM0894

Alternate gene names: 15602759

Gene position: 1051576-1049678 (Counterclockwise)

Preceding gene: 15602760

Following gene: 15602758

Centisome position: 46.58

GC content: 45.5

Gene sequence:

>1899_bases
ATGTCAAAACAAATTCAAGTACCAGATATTGGTGGTGATGAAGTGACTGTGACAGAAGTCATGGTAAAAGCGGGTGATAC
GGTTGCAGTTGATCAAAGCGTAATCAATGTAGAAGGTGATAAAGCCTCAATGGAAGTCCCTTCACCAGAAGCGGGTGTGA
TCAAAGAAGTGTTAGTGAAAGTGGGTGACAAAGTGTCAACGGGTTCACCGATGTTTGTGTTAGAAACGGGTGATGCAAAA
CCTGCTGAAACGCCAAAAGCTGAAACAGCACCAGTATCTGCGCCAGCGCCAGCAGCAAGCGCAGTGGTTGAAGTACATGT
CCCCGATATTGGTGGTGATGAAGTTAACGTGACAGAAATTATGGTCAAAGTGGGTGATAAAGTTGAGGTTGAGCAATCGA
TCATCAATGTAGAAGGTGATAAAGCCTCAATGGAAGTCCCAGCCCCAATCGCAGGTGTTGTTAAAGAAATTCTAATCAAT
GTTGGTGACAAAGTATCAACAGGATCATTGATTATGAAATTTGAAACGGGTGATGCGCCAGCAGTAGCGACACCAGCACA
AGCGCCAACAGCTGCGCCATCAGCACCTGCACAAAGTGCGGTAAAAGATGTGAACGTGCCAGATATTGGTGGTGACGAAG
TCAACGTGACGGAAATCATGGTGAACGTGGGTGACACGATCACAGAAGAACAATCATTAATTACTGTTGAAGGTGATAAA
GCCTCAATGGAAGTGCCTGCGCCATTTGCCGGTGTGGTAAAAGAAATTTTAGTGAAATCTGGCGACAAAGTCTCTACGGG
TTCATTAATTATGCGCTTTGAAGTAGCGGGAGCCGCACCTTCAGCAGCACCAGCACAACCTGCACCTGCTGCACAAGCGG
CAGCACCACAAGCAGCAACGCCAGCACCTGTTGCAAGCGCGCCAGCGGGTGATGCAGAAGTAACAGGTAGTTCTGTATTT
GCTTATGCCACACCGGTTGTACGTCGTTTAGCACGTGAATTTGGTGTGAACCTCGATAAAGTGAAAGGTACTGGTCGTAA
AGGTCGTATCTTAAAAGAAGACGTTCAAGCATATGTGAAAGCAGCAATCAAAGCGGTTGAATCAGGTTCTGTATCTGCAG
CGCCAGCGGGCAGCGGTGTAGCAAATGGTGCAGGCTTAGGCTTATTGCCATGGCCGAAAGTGGATTTCAGCAAGTTTGGT
GAAGTGGAAGAAGTAGAACTCACCCGTATTAACAAAATTTCGGGTGCAAACTTACACCGTAACTGGGTGATGATCCCGCA
TGTTACACATTTCGATCGTGCCGATATCACTGACTTAGAAGCGTTCCGTAAAGAACAAAATGTGTTAGCGGAAAAACAAA
AATGGGGTGTGAAAATCACGCCAGTGGTGTTTATCATGAAAGCGGCTGCCAAAGCATTAGAAGCGTATCCACGTTTCAAT
AGCTCAATTTCTGAAGATGGTCAACGTTTAACCTTGAAAAAATATGTCAATATTGGTGTGGCAGTGGATACACCAAACGG
CTTAGTCGTGCCTGTCTTTAAAGATGTCAACAAAAAAGGCATTATCGAACTTTCTCGTGAATTAATGGAAGTGTCGAAAA
AAGCACGTGACGGCAAATTAACTGCTTCAGATATGCAAGGGGGTTGTTTCACGATTTCAAGCTTAGGGGGCTTAGGTACG
ACACACTTTGCGCCAATCGTGAATGCGCCAGAAGTCGCAATTCTAGGTGTGTCTAAATCAGAAATGGCGCCAGTTTGGAA
CGGTAAAGAATTTACGCCACGCTTAATGTTGCCATTATCATTGTCTTTCGACCACCGTGTGATCGATGGTGCGGATGGCG
CACGTTTCATTAGCTATATCAATGGCGTGTTATCTGATCTTCGTCGTTTAGTTATGTAA

Upstream 100 bases:

>100_bases
AGATAAAGTGAACCCACTTTACGCCTAAGTAAATAGGATTAACATTCCAGCTCCCTGATATCAGGGAGCATGTTATGGAA
TGATTGGAGGATAAAAAAAC

Downstream 100 bases:

>100_bases
TCGCACTGTCCGTTCGGTAAATTGCCGAGCGGACATCATTAATCAACCCAAATGTGGTGTGAAAGTGCGGTCTAAAATCG
CCTATCTTTTGATAAAATGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MSKQIQVPDIGGDEVTVTEVMVKAGDTVAVDQSVINVEGDKASMEVPSPEAGVIKEVLVKVGDKVSTGSPMFVLETGDAK
PAETPKAETAPVSAPAPAASAVVEVHVPDIGGDEVNVTEIMVKVGDKVEVEQSIINVEGDKASMEVPAPIAGVVKEILIN
VGDKVSTGSLIMKFETGDAPAVATPAQAPTAAPSAPAQSAVKDVNVPDIGGDEVNVTEIMVNVGDTITEEQSLITVEGDK
ASMEVPAPFAGVVKEILVKSGDKVSTGSLIMRFEVAGAAPSAAPAQPAPAAQAAAPQAATPAPVASAPAGDAEVTGSSVF
AYATPVVRRLAREFGVNLDKVKGTGRKGRILKEDVQAYVKAAIKAVESGSVSAAPAGSGVANGAGLGLLPWPKVDFSKFG
EVEEVELTRINKISGANLHRNWVMIPHVTHFDRADITDLEAFRKEQNVLAEKQKWGVKITPVVFIMKAAAKALEAYPRFN
SSISEDGQRLTLKKYVNIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMEVSKKARDGKLTASDMQGGCFTISSLGGLGT
THFAPIVNAPEVAILGVSKSEMAPVWNGKEFTPRLMLPLSLSFDHRVIDGADGARFISYINGVLSDLRRLVM

Sequences:

>Translated_632_residues
MSKQIQVPDIGGDEVTVTEVMVKAGDTVAVDQSVINVEGDKASMEVPSPEAGVIKEVLVKVGDKVSTGSPMFVLETGDAK
PAETPKAETAPVSAPAPAASAVVEVHVPDIGGDEVNVTEIMVKVGDKVEVEQSIINVEGDKASMEVPAPIAGVVKEILIN
VGDKVSTGSLIMKFETGDAPAVATPAQAPTAAPSAPAQSAVKDVNVPDIGGDEVNVTEIMVNVGDTITEEQSLITVEGDK
ASMEVPAPFAGVVKEILVKSGDKVSTGSLIMRFEVAGAAPSAAPAQPAPAAQAAAPQAATPAPVASAPAGDAEVTGSSVF
AYATPVVRRLAREFGVNLDKVKGTGRKGRILKEDVQAYVKAAIKAVESGSVSAAPAGSGVANGAGLGLLPWPKVDFSKFG
EVEEVELTRINKISGANLHRNWVMIPHVTHFDRADITDLEAFRKEQNVLAEKQKWGVKITPVVFIMKAAAKALEAYPRFN
SSISEDGQRLTLKKYVNIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMEVSKKARDGKLTASDMQGGCFTISSLGGLGT
THFAPIVNAPEVAILGVSKSEMAPVWNGKEFTPRLMLPLSLSFDHRVIDGADGARFISYINGVLSDLRRLVM
>Mature_631_residues
SKQIQVPDIGGDEVTVTEVMVKAGDTVAVDQSVINVEGDKASMEVPSPEAGVIKEVLVKVGDKVSTGSPMFVLETGDAKP
AETPKAETAPVSAPAPAASAVVEVHVPDIGGDEVNVTEIMVKVGDKVEVEQSIINVEGDKASMEVPAPIAGVVKEILINV
GDKVSTGSLIMKFETGDAPAVATPAQAPTAAPSAPAQSAVKDVNVPDIGGDEVNVTEIMVNVGDTITEEQSLITVEGDKA
SMEVPAPFAGVVKEILVKSGDKVSTGSLIMRFEVAGAAPSAAPAQPAPAAQAAAPQAATPAPVASAPAGDAEVTGSSVFA
YATPVVRRLAREFGVNLDKVKGTGRKGRILKEDVQAYVKAAIKAVESGSVSAAPAGSGVANGAGLGLLPWPKVDFSKFGE
VEEVELTRINKISGANLHRNWVMIPHVTHFDRADITDLEAFRKEQNVLAEKQKWGVKITPVVFIMKAAAKALEAYPRFNS
SISEDGQRLTLKKYVNIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMEVSKKARDGKLTASDMQGGCFTISSLGGLGTT
HFAPIVNAPEVAILGVSKSEMAPVWNGKEFTPRLMLPLSLSFDHRVIDGADGARFISYINGVLSDLRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=31.322505800464, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI31711992, Length=423, Percent_Identity=30.2600472813239, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI19923748, Length=209, Percent_Identity=34.4497607655502, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI203098816, Length=485, Percent_Identity=28.0412371134021, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=27.9279279279279, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.2564102564103, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786305, Length=639, Percent_Identity=70.735524256651, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=29.9270072992701, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=407, Percent_Identity=31.2039312039312, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=29.5871559633028, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=209, Percent_Identity=36.3636363636364, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=31.8327974276527, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=27.4881516587678, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=427, Percent_Identity=27.8688524590164, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=31.4420803782506, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=34.375, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=31.4893617021277, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65966; Mature: 65835

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQIQVPDIGGDEVTVTEVMVKAGDTVAVDQSVINVEGDKASMEVPSPEAGVIKEVLVK
CCCCEECCCCCCCCEEHHHHHHHCCCEEEECCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGSPMFVLETGDAKPAETPKAETAPVSAPAPAASAVVEVHVPDIGGDEVNVTEI
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHH
MVKVGDKVEVEQSIINVEGDKASMEVPAPIAGVVKEILINVGDKVSTGSLIMKFETGDAP
EECCCCCEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
AVATPAQAPTAAPSAPAQSAVKDVNVPDIGGDEVNVTEIMVNVGDTITEEQSLITVEGDK
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEHEEEEEECCCCCCCCCCEEEEECCC
ASMEVPAPFAGVVKEILVKSGDKVSTGSLIMRFEVAGAAPSAAPAQPAPAAQAAAPQAAT
CCEECCCCHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCC
PAPVASAPAGDAEVTGSSVFAYATPVVRRLAREFGVNLDKVKGTGRKGRILKEDVQAYVK
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCHHHHHHHHHHH
AAIKAVESGSVSAAPAGSGVANGAGLGLLPWPKVDFSKFGEVEEVELTRINKISGANLHR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEHHHECCCCCCCCCC
NWVMIPHVTHFDRADITDLEAFRKEQNVLAEKQKWGVKITPVVFIMKAAAKALEAYPRFN
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCC
SSISEDGQRLTLKKYVNIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMEVSKKARDGKL
CCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
TASDMQGGCFTISSLGGLGTTHFAPIVNAPEVAILGVSKSEMAPVWNGKEFTPRLMLPLS
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEE
LSFDHRVIDGADGARFISYINGVLSDLRRLVM
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKQIQVPDIGGDEVTVTEVMVKAGDTVAVDQSVINVEGDKASMEVPSPEAGVIKEVLVK
CCCEECCCCCCCCEEHHHHHHHCCCEEEECCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGSPMFVLETGDAKPAETPKAETAPVSAPAPAASAVVEVHVPDIGGDEVNVTEI
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHH
MVKVGDKVEVEQSIINVEGDKASMEVPAPIAGVVKEILINVGDKVSTGSLIMKFETGDAP
EECCCCCEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
AVATPAQAPTAAPSAPAQSAVKDVNVPDIGGDEVNVTEIMVNVGDTITEEQSLITVEGDK
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEHEEEEEECCCCCCCCCCEEEEECCC
ASMEVPAPFAGVVKEILVKSGDKVSTGSLIMRFEVAGAAPSAAPAQPAPAAQAAAPQAAT
CCEECCCCHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCC
PAPVASAPAGDAEVTGSSVFAYATPVVRRLAREFGVNLDKVKGTGRKGRILKEDVQAYVK
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCHHHHHHHHHHH
AAIKAVESGSVSAAPAGSGVANGAGLGLLPWPKVDFSKFGEVEEVELTRINKISGANLHR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEHHHECCCCCCCCCC
NWVMIPHVTHFDRADITDLEAFRKEQNVLAEKQKWGVKITPVVFIMKAAAKALEAYPRFN
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCC
SSISEDGQRLTLKKYVNIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMEVSKKARDGKL
CCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
TASDMQGGCFTISSLGGLGTTHFAPIVNAPEVAILGVSKSEMAPVWNGKEFTPRLMLPLS
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEE
LSFDHRVIDGADGARFISYINGVLSDLRRLVM
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]