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The map label for this gene is lpdA [H]

Identifier: 15602758

GI number: 15602758

Start: 1048135

End: 1049553

Strand: Reverse

Name: lpdA [H]

Synonym: PM0893

Alternate gene names: 15602758

Gene position: 1049553-1048135 (Counterclockwise)

Preceding gene: 15602759

Following gene: 15602756

Centisome position: 46.49

GC content: 43.06

Gene sequence:

>1419_bases
ATGAATAAAGAAATTAAAACTCAGGTTGTGGTATTAGGTGCGGGTCCTGCGGGCTATTCCGCCGCATTCCGTTGTGCAGA
TTTAGGTTTAGAAACCGTGATTGTTGAACGTTATTCTACGTTAGGTGGCGTATGTTTAAACGTGGGTTGTATCCCGTCAA
AAGCCTTATTACACGTGGCGAAAGTGATTGAAGAAGCCAAAGCGCTTGCAGAGCATGGTATCGTGTTCGGTGAGCCAAGC
ACAGATGTGAACAAAATCCGTGGTTGGAAAGAAAAAGTCATTAACCAGTTAACGGGTGGTTTAGCGGGTATGGCTAAACA
ACGTAAAGTGCAAGTAGTGAACGGCTATGGTAAATTCTCTGGTCCAAATACGATTATTGTTGCGGGCGAAGAAGGGGAAA
CGACGATCAAGTTTGATCATGCGATTATTGCGGCAGGATCGCGTCCAATTCAATTACCATTTATTCCACATGAAGATCCA
CGTATTTGGGATTCCACAGATGCACTTAAATTAAAAGAAGTGCCGGAGAATTTACTTATCATGGGTGGTGGTATTATCGG
TCTAGAAATGGGAACGGTTTACCATGCATTAGGCTCAAAAATTGATGTAGTCGAAATGTTTGACCAAGTGATCCCGGCTG
CGGATAAAGACATGGTTCAAATCTACACCAAACGCGTCGCGAAGAAATTCAATTTATTATTAGAAACGAAAGTGACTGCG
GTTGAAGCAAAAGAAGATGGTATTTATGTTTCAATGGAAGGTAAAGCTTCTGAAACTCGTCGTTATGATGCTGTATTAGT
GGCGATTGGTCGTGTACCAAATGGTAAATTGATTGATGCCGGTGTTGCGGGTGTTGAAGTAGATGATCGTGGTTTCATTC
GTACCGACAAACAAATGCGTACCAATGTGCCTCACATTTTCGCCATTGGTGATATCGTGGGTCAACCAATGTTAGCACAC
AAAGGTGTTCATGAAGGTCACGTCGCAGCAGAAGTGATTGCAGGCATGAAACATTACTTCGATCCAAAAGTGATTCCATC
TATTGCGTATACTGAACCTGAAGTGGCTTGGGTGGGTAAAACTGAGAAAGAATGTAAAGCGGAAGGTATCGCTTATGAAG
TAGCGAAATTCCCTTGGGCTGCCTCAGGTCGTGCGATTGCATCTGACTGTGCAGATGGCATGACAAAATTGATCTTTGAT
AAAAATACACATCGTGTTATCGGTGGGGCAATCGTCGGGACGAATGGTGGTGAATTGTTAGGTGAAATCGGTCTTGCTAT
CGAAATGGGTTGTGATGCGGAAGATTTAGCCTTAACGATCCATGCTCACCCAACATTACACGAGTCTGTTGGTCTTGCAG
CAGAAGTATTTGAAGGGTCAATTACTGACTTACCAAATCCAAAAGCGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
CCGAGCGGACATCATTAATCAACCCAAATGTGGTGTGAAAGTGCGGTCTAAAATCGCCTATCTTTTGATAAAATGCATCG
CATTCTTTAACGAGGTTAAA

Downstream 100 bases:

>100_bases
TACGTTTTTGTTGAAACTGATAAGTAACAAAAGTTTTTAAACGAAATAGCTGTGCGGTTAAACTTTTAAAAGTTTCACCG
CACTTTTTTATTTATGAGAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPS
TDVNKIRGWKEKVINQLTGGLAGMAKQRKVQVVNGYGKFSGPNTIIVAGEEGETTIKFDHAIIAAGSRPIQLPFIPHEDP
RIWDSTDALKLKEVPENLLIMGGGIIGLEMGTVYHALGSKIDVVEMFDQVIPAADKDMVQIYTKRVAKKFNLLLETKVTA
VEAKEDGIYVSMEGKASETRRYDAVLVAIGRVPNGKLIDAGVAGVEVDDRGFIRTDKQMRTNVPHIFAIGDIVGQPMLAH
KGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKAEGIAYEVAKFPWAASGRAIASDCADGMTKLIFD
KNTHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK

Sequences:

>Translated_472_residues
MNKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPS
TDVNKIRGWKEKVINQLTGGLAGMAKQRKVQVVNGYGKFSGPNTIIVAGEEGETTIKFDHAIIAAGSRPIQLPFIPHEDP
RIWDSTDALKLKEVPENLLIMGGGIIGLEMGTVYHALGSKIDVVEMFDQVIPAADKDMVQIYTKRVAKKFNLLLETKVTA
VEAKEDGIYVSMEGKASETRRYDAVLVAIGRVPNGKLIDAGVAGVEVDDRGFIRTDKQMRTNVPHIFAIGDIVGQPMLAH
KGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKAEGIAYEVAKFPWAASGRAIASDCADGMTKLIFD
KNTHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
>Mature_472_residues
MNKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPS
TDVNKIRGWKEKVINQLTGGLAGMAKQRKVQVVNGYGKFSGPNTIIVAGEEGETTIKFDHAIIAAGSRPIQLPFIPHEDP
RIWDSTDALKLKEVPENLLIMGGGIIGLEMGTVYHALGSKIDVVEMFDQVIPAADKDMVQIYTKRVAKKFNLLLETKVTA
VEAKEDGIYVSMEGKASETRRYDAVLVAIGRVPNGKLIDAGVAGVEVDDRGFIRTDKQMRTNVPHIFAIGDIVGQPMLAH
KGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKAEGIAYEVAKFPWAASGRAIASDCADGMTKLIFD
KNTHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=42.4507658643326, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=27.3730684326711, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=28.0373831775701, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=27.9176201372998, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=27.9176201372998, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=27.9176201372998, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=27.9176201372998, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=27.9176201372998, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=27.816091954023, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=26.3529411764706, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=88.6075949367088, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=29.0672451193059, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=27.8145695364238, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=28.125, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=39.9553571428571, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=27.8242677824268, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=438, Percent_Identity=26.027397260274, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=437, Percent_Identity=24.7139588100687, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=24.7139588100687, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.2280701754386, Blast_Score=303, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.4957264957265, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=452, Percent_Identity=26.1061946902655, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21358499, Length=450, Percent_Identity=41.3333333333333, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=28.6016949152542, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=28.8172043010753, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=28.8172043010753, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=25.369978858351, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50501; Mature: 50501

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPSTDVNKIRGWKEKVINQLTGGLAGMAKQRKVQVVNGYGKFS
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCC
GPNTIIVAGEEGETTIKFDHAIIAAGSRPIQLPFIPHEDPRIWDSTDALKLKEVPENLLI
CCCEEEEECCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEEHHCCCCCEEE
MGGGIIGLEMGTVYHALGSKIDVVEMFDQVIPAADKDMVQIYTKRVAKKFNLLLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
VEAKEDGIYVSMEGKASETRRYDAVLVAIGRVPNGKLIDAGVAGVEVDDRGFIRTDKQMR
EEECCCCEEEEECCCCCHHHHHEEEEEEEECCCCCCEEECCCCEEEECCCCCEECCHHHH
TNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGK
CCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECC
TEKECKAEGIAYEVAKFPWAASGRAIASDCADGMTKLIFDKNTHRVIGGAIVGTNGGELL
CHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHH
GEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
HHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPSTDVNKIRGWKEKVINQLTGGLAGMAKQRKVQVVNGYGKFS
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCC
GPNTIIVAGEEGETTIKFDHAIIAAGSRPIQLPFIPHEDPRIWDSTDALKLKEVPENLLI
CCCEEEEECCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEEHHCCCCCEEE
MGGGIIGLEMGTVYHALGSKIDVVEMFDQVIPAADKDMVQIYTKRVAKKFNLLLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
VEAKEDGIYVSMEGKASETRRYDAVLVAIGRVPNGKLIDAGVAGVEVDDRGFIRTDKQMR
EEECCCCEEEEECCCCCHHHHHEEEEEEEECCCCCCEEECCCCEEEECCCCCEECCHHHH
TNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGK
CCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECC
TEKECKAEGIAYEVAKFPWAASGRAIASDCADGMTKLIFDKNTHRVIGGAIVGTNGGELL
CHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHH
GEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
HHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]