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The map label for this gene is rfaE

Identifier: 15602749

GI number: 15602749

Start: 1039136

End: 1040566

Strand: Reverse

Name: rfaE

Synonym: PM0884

Alternate gene names: 15602749

Gene position: 1040566-1039136 (Counterclockwise)

Preceding gene: 15602751

Following gene: 15602745

Centisome position: 46.09

GC content: 42.7

Gene sequence:

>1431_bases
ATGTTTCAATATTCCGCAGAATTTAAACAAGCAAAAGTGTTGGTATTGGGCGATGTAATGCTTGATCGTTATTGGTTTGG
GGCGACAAATCGCATTTCACCAGAAGCACCGGTTCCTGTGGTACGTGTACAAGATAACGAAGAACGTGCAGGTGGGGCAG
CCAACGTTGCGATGAATATCGCTGCGTTAAATGTGCCAGTAAAATTATTGGGCTTAACGGGACGAGATGAAACCGGTCTT
GCCTTAGAAAGTTTATTAGCCAAAAGCCGTATTGAATGTGATTTTGTACAATTACATACTCATCCTACCATTACAAAATT
ACGTATTTTGTCCCGCCATCAACAATTATTACGCTTAGATTTTGAAGAAGACTTCCAAAATGTGACGAGCGATGCGTTAT
TGCAAAAATTAGACAGTGCGTTACAAAATTATGGCGCATTAGTGTTATCCGATTATGGTAAAGGCACCTTAAATCAAGTG
CAAAAAATGATTCAACTTGCACGCCAAGCCAATATCCCCGTGTTAATTGATCCGAAAGGAACCGATTTTGAACGTTATCG
TGGTGCAACCTTATTAACACCGAATATGTCTGAATTTGAAGCGGTGGTTGGGAAGTGTGATTCTGAAGAAGAGATTATCG
AAAAAGGGTTGAAATTAATTGAGCAGATCGACCTGAGTGCGCTTTTAGTGACACGTTCTGAAAAAGGCATGACGTTATTG
CGTCCAAATCAACCGGCATTTCATTTAGCCACCGAAGCGAAAGAAGTATTTGATGTGACTGGGGCGGGAGATACGGTGAT
TAGTGTGTTAGCCACAGGCTTAGCCGATGGTCGTTCGTTTGAAGAAGCCTGCTATTTAGCCAATGTCGCAGCAGGGATTG
TCGTGGGTAAATTAGGGACCTCAACCGTTTCTACGGTGGAATTAGAAAATGCAATTCACGGTCGTTCTAATACCGGATTT
GGTATTATGAGCGAAGCCGAACTGAAACGCGTAGTGGCGCAAGCGAAGGCGCGTGGTGAGAAAATTGTGATGACTAATGG
CTGTTTTGACATTCTTCACCCGGGACATGTGTCTTACCTCGAAAACGCACGTAAATTGGGCGATCGCTTAATTGTCGCAG
TGAACAGCGATGACTCAGTCAAACGCCTAAAAGGTGAAACCCGCCCAATTAATGACCTTCAATCTCGTATGGCAGTATTG
GCAGGCTTATCTTCAGTCGATTGGTTAGTGGCGTTCCATGAAGATACACCACAACGTTTAATTGCTGAAGTATTACCTGA
TTTATTGGTGAAAGGTGGCGATTACAAACCAGAAGACATCGCCGGTAGCCAAGAAGTTTGGGCGAGTGGTGGTGATGTAA
AAGTATTAAACTTTGAAAATGGTTGTTCAACCAGTAATGTGATCAGTAAGATTCAGCAGTTAAAAGATTAA

Upstream 100 bases:

>100_bases
AGTCATGTAATAAAAAGTCGTGCAATTTATTGGATTTTAAGGGGAAATTGGTGTTTTCCTGTGCTAAAATTAAGTAATTT
TTTATTAAGAGAGAACGAAA

Downstream 100 bases:

>100_bases
GATTGAATTTGATTTCATCATCATACCTATTTGGGGAAAGGGCAAACAGTTATGTTTGCCTTTTTTATGAGATTAAAACA
GGGTCGTGTTATCAATTATG

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MFQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQDNEERAGGAANVAMNIAALNVPVKLLGLTGRDETGL
ALESLLAKSRIECDFVQLHTHPTITKLRILSRHQQLLRLDFEEDFQNVTSDALLQKLDSALQNYGALVLSDYGKGTLNQV
QKMIQLARQANIPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCDSEEEIIEKGLKLIEQIDLSALLVTRSEKGMTLL
RPNQPAFHLATEAKEVFDVTGAGDTVISVLATGLADGRSFEEACYLANVAAGIVVGKLGTSTVSTVELENAIHGRSNTGF
GIMSEAELKRVVAQAKARGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNSDDSVKRLKGETRPINDLQSRMAVL
AGLSSVDWLVAFHEDTPQRLIAEVLPDLLVKGGDYKPEDIAGSQEVWASGGDVKVLNFENGCSTSNVISKIQQLKD

Sequences:

>Translated_476_residues
MFQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQDNEERAGGAANVAMNIAALNVPVKLLGLTGRDETGL
ALESLLAKSRIECDFVQLHTHPTITKLRILSRHQQLLRLDFEEDFQNVTSDALLQKLDSALQNYGALVLSDYGKGTLNQV
QKMIQLARQANIPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCDSEEEIIEKGLKLIEQIDLSALLVTRSEKGMTLL
RPNQPAFHLATEAKEVFDVTGAGDTVISVLATGLADGRSFEEACYLANVAAGIVVGKLGTSTVSTVELENAIHGRSNTGF
GIMSEAELKRVVAQAKARGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNSDDSVKRLKGETRPINDLQSRMAVL
AGLSSVDWLVAFHEDTPQRLIAEVLPDLLVKGGDYKPEDIAGSQEVWASGGDVKVLNFENGCSTSNVISKIQQLKD
>Mature_476_residues
MFQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQDNEERAGGAANVAMNIAALNVPVKLLGLTGRDETGL
ALESLLAKSRIECDFVQLHTHPTITKLRILSRHQQLLRLDFEEDFQNVTSDALLQKLDSALQNYGALVLSDYGKGTLNQV
QKMIQLARQANIPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCDSEEEIIEKGLKLIEQIDLSALLVTRSEKGMTLL
RPNQPAFHLATEAKEVFDVTGAGDTVISVLATGLADGRSFEEACYLANVAAGIVVGKLGTSTVSTVELENAIHGRSNTGF
GIMSEAELKRVVAQAKARGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNSDDSVKRLKGETRPINDLQSRMAVL
AGLSSVDWLVAFHEDTPQRLIAEVLPDLLVKGGDYKPEDIAGSQEVWASGGDVKVLNFENGCSTSNVISKIQQLKD

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=469, Percent_Identity=69.7228144989339, Blast_Score=689, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_PASMU (Q9CME6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245821.1
- ProteinModelPortal:   Q9CME6
- SMR:   Q9CME6
- GeneID:   1244231
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0884
- NMPDR:   fig|272843.1.peg.884
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- BioCyc:   PMUL272843:PM0884-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51885; Mature: 51885

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQDNEERAGGAANVAMNI
CCCCCCCHHHEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHEEEE
AALNVPVKLLGLTGRDETGLALESLLAKSRIECDFVQLHTHPTITKLRILSRHQQLLRLD
EEEECCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
FEEDFQNVTSDALLQKLDSALQNYGALVLSDYGKGTLNQVQKMIQLARQANIPVLIDPKG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNMSEFEAVVGKCDSEEEIIEKGLKLIEQIDLSALLVTRSEKGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
RPNQPAFHLATEAKEVFDVTGAGDTVISVLATGLADGRSFEEACYLANVAAGIVVGKLGT
CCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCC
STVSTVELENAIHGRSNTGFGIMSEAELKRVVAQAKARGEKIVMTNGCFDILHPGHVSYL
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHH
ENARKLGDRLIVAVNSDDSVKRLKGETRPINDLQSRMAVLAGLSSVDWLVAFHEDTPQRL
HHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
IAEVLPDLLVKGGDYKPEDIAGSQEVWASGGDVKVLNFENGCSTSNVISKIQQLKD
HHHHHHHHHHCCCCCCCHHCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQDNEERAGGAANVAMNI
CCCCCCCHHHEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHEEEE
AALNVPVKLLGLTGRDETGLALESLLAKSRIECDFVQLHTHPTITKLRILSRHQQLLRLD
EEEECCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
FEEDFQNVTSDALLQKLDSALQNYGALVLSDYGKGTLNQVQKMIQLARQANIPVLIDPKG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNMSEFEAVVGKCDSEEEIIEKGLKLIEQIDLSALLVTRSEKGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
RPNQPAFHLATEAKEVFDVTGAGDTVISVLATGLADGRSFEEACYLANVAAGIVVGKLGT
CCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCC
STVSTVELENAIHGRSNTGFGIMSEAELKRVVAQAKARGEKIVMTNGCFDILHPGHVSYL
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHH
ENARKLGDRLIVAVNSDDSVKRLKGETRPINDLQSRMAVLAGLSSVDWLVAFHEDTPQRL
HHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
IAEVLPDLLVKGGDYKPEDIAGSQEVWASGGDVKVLNFENGCSTSNVISKIQQLKD
HHHHHHHHHHCCCCCCCHHCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100