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The map label for this gene is nifS_2

Identifier: 15602747

GI number: 15602747

Start: 1037075

End: 1038340

Strand: Direct

Name: nifS_2

Synonym: PM0882

Alternate gene names: 15602747

Gene position: 1037075-1038340 (Clockwise)

Preceding gene: 15602746

Following gene: 15602748

Centisome position: 45.94

GC content: 43.52

Gene sequence:

>1266_bases
TTGGATTATGGCAAGACACTCACCCAATCCCTCCTGAGCAATGGCGCAGACAAAAGAGAAAATAAGATGGCATTTAATCC
AAGTGTATTTAAACAACATTTTCCTTACCTCCAACAAGCGGATGCAGTTGTCTATTTAGACAGTGCTGCCACGGCATTAA
AGCCACAAGTGCTGACTGATGCGACGATTGCCTTTTATCAATCTGCTGGATCCGTACATCGTAGTCAATATGATGAAAAA
CAGACCGCACTTTTTGAACAAGCAAGAGAAAATGTGAAAAACTTCATTGGTGCAGAATCTGAAGAGACAATTATTTGGAC
ATCGGGGACAACGCATGCTATCAATTGCGTTGCAAGAGGGTTATCTCATCAACTGCATCCTAAAGCTGAAATTATCATTA
GCGAAGCGGACCATCATGCTAATTTTGTTACTTGGTCTGAAATCGCGCGTCAATATGGCGCAACCTTGCATATTTTACCG
ATTAATGAGCAATGGTTGATTGAAGAACAGGATCTTATTGCTGTACTCAATACCAATACCGTATTGGTTGCATTGAATAT
GGTTTCTAATGTCACTGGAACGGAGCAACCTGTTGCCAATTTAATTAAACTGATTCGACAACACAGTCATGCGTTGGTAC
TCGTCGACGCGGCACAAGCGATTAGCCATTTGCCTATCGACTTACAAAGGTTAGATGCGGATTTTATTGCCTTCTCAGCG
CATAAACTCTATGGTCCGAATGGACTTGGTGTGTTAAGTGGGAAACGACATGCCCTTGAACAACTTCATCCCCTGTTGTA
CGGAGGGAAAATGGTAGAACGAGTATCTGCACAACACATCCGTTTTGCCGAACTACCTTATCGCCTTGAGGCTGGCACGC
CAAATATTGCAGGTGTGATTGGTTTTAATGCTGTCTTAGAATGGTTAAGCCAGTGGGACTTAACTGCTGCCGAGCAACAT
GCCATTGCTTTGGCAGAGCAATGTAAAATGCGATTAAAAAATTACCCACACTGCCAGTTATTTTTATCACCGCAACCGAG
CAGCATCGTTTGTTTTGTGTTTAACGGTATAGCCACCTCTGACATCGCTACATTGCTCGCTGAACAAAATATAGCCTTAC
GCGCAGGTGAACACTGCGCCCAACCTTATTTAGCGCGCTTAGGACAGCACAGCACTTTGCGGCTGTCCTTTGCACCTTAT
AACCAACAAGCGGATGTTGATGCGTTTTTCTCTGCCCTTGACAACGCTTTAGCCTTATTAGATTAA

Upstream 100 bases:

>100_bases
AATATCGAAATGGTGAAATCTGGTATGGCTTGGGCATATCCGCGTTATAATCACGATCCAATGTATTTGCGCGCGCAACA
AGCCGCCATGAAAGCCCGTC

Downstream 100 bases:

>100_bases
ATGATGAAAGAACAACTCCTTAACGCAAAAAGCTGGGAAGAACGTTACCGCCTGATTATCCAAGCCGGCAAACAGCTACC
ATTCCCCACAGAACAACAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDYGKTLTQSLLSNGADKRENKMAFNPSVFKQHFPYLQQADAVVYLDSAATALKPQVLTDATIAFYQSAGSVHRSQYDEK
QTALFEQARENVKNFIGAESEETIIWTSGTTHAINCVARGLSHQLHPKAEIIISEADHHANFVTWSEIARQYGATLHILP
INEQWLIEEQDLIAVLNTNTVLVALNMVSNVTGTEQPVANLIKLIRQHSHALVLVDAAQAISHLPIDLQRLDADFIAFSA
HKLYGPNGLGVLSGKRHALEQLHPLLYGGKMVERVSAQHIRFAELPYRLEAGTPNIAGVIGFNAVLEWLSQWDLTAAEQH
AIALAEQCKMRLKNYPHCQLFLSPQPSSIVCFVFNGIATSDIATLLAEQNIALRAGEHCAQPYLARLGQHSTLRLSFAPY
NQQADVDAFFSALDNALALLD

Sequences:

>Translated_421_residues
MDYGKTLTQSLLSNGADKRENKMAFNPSVFKQHFPYLQQADAVVYLDSAATALKPQVLTDATIAFYQSAGSVHRSQYDEK
QTALFEQARENVKNFIGAESEETIIWTSGTTHAINCVARGLSHQLHPKAEIIISEADHHANFVTWSEIARQYGATLHILP
INEQWLIEEQDLIAVLNTNTVLVALNMVSNVTGTEQPVANLIKLIRQHSHALVLVDAAQAISHLPIDLQRLDADFIAFSA
HKLYGPNGLGVLSGKRHALEQLHPLLYGGKMVERVSAQHIRFAELPYRLEAGTPNIAGVIGFNAVLEWLSQWDLTAAEQH
AIALAEQCKMRLKNYPHCQLFLSPQPSSIVCFVFNGIATSDIATLLAEQNIALRAGEHCAQPYLARLGQHSTLRLSFAPY
NQQADVDAFFSALDNALALLD
>Mature_421_residues
MDYGKTLTQSLLSNGADKRENKMAFNPSVFKQHFPYLQQADAVVYLDSAATALKPQVLTDATIAFYQSAGSVHRSQYDEK
QTALFEQARENVKNFIGAESEETIIWTSGTTHAINCVARGLSHQLHPKAEIIISEADHHANFVTWSEIARQYGATLHILP
INEQWLIEEQDLIAVLNTNTVLVALNMVSNVTGTEQPVANLIKLIRQHSHALVLVDAAQAISHLPIDLQRLDADFIAFSA
HKLYGPNGLGVLSGKRHALEQLHPLLYGGKMVERVSAQHIRFAELPYRLEAGTPNIAGVIGFNAVLEWLSQWDLTAAEQH
AIALAEQCKMRLKNYPHCQLFLSPQPSSIVCFVFNGIATSDIATLLAEQNIALRAGEHCAQPYLARLGQHSTLRLSFAPY
NQQADVDAFFSALDNALALLD

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=380, Percent_Identity=25, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI156713448, Length=436, Percent_Identity=25, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=50.1246882793017, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1787970, Length=406, Percent_Identity=35.7142857142857, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI48994898, Length=400, Percent_Identity=24.5, Blast_Score=105, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=232, Percent_Identity=30.6034482758621, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193211090, Length=280, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319831, Length=393, Percent_Identity=26.972010178117, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20129463, Length=380, Percent_Identity=27.3684210526316, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSD_PASMU (P57989)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245819.1
- ProteinModelPortal:   P57989
- SMR:   P57989
- GeneID:   1244229
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0882
- NMPDR:   fig|272843.1.peg.882
- HOGENOM:   HBG635316
- OMA:   NIRILAE
- ProtClustDB:   CLSK870284
- BioCyc:   PMUL272843:PM0882-MONOMER
- BRENDA:   2.8.1.7
- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 46490; Mature: 46490

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYGKTLTQSLLSNGADKRENKMAFNPSVFKQHFPYLQQADAVVYLDSAATALKPQVLTD
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHCCEEEEECCCHHHHCCHHHHH
ATIAFYQSAGSVHRSQYDEKQTALFEQARENVKNFIGAESEETIIWTSGTTHAINCVARG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
LSHQLHPKAEIIISEADHHANFVTWSEIARQYGATLHILPINEQWLIEEQDLIAVLNTNT
HHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHCCEEEEEECCCCEEEECCCEEEEEECCE
VLVALNMVSNVTGTEQPVANLIKLIRQHSHALVLVDAAQAISHLPIDLQRLDADFIAFSA
EEEEEEHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHCCCHHHEEEH
HKLYGPNGLGVLSGKRHALEQLHPLLYGGKMVERVSAQHIRFAELPYRLEAGTPNIAGVI
HHEECCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCEEEECCCCCCCHHH
GFNAVLEWLSQWDLTAAEQHAIALAEQCKMRLKNYPHCQLFLSPQPSSIVCFVFNGIATS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHH
DIATLLAEQNIALRAGEHCAQPYLARLGQHSTLRLSFAPYNQQADVDAFFSALDNALALL
HHHHHHHCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
D
C
>Mature Secondary Structure
MDYGKTLTQSLLSNGADKRENKMAFNPSVFKQHFPYLQQADAVVYLDSAATALKPQVLTD
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHCCEEEEECCCHHHHCCHHHHH
ATIAFYQSAGSVHRSQYDEKQTALFEQARENVKNFIGAESEETIIWTSGTTHAINCVARG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
LSHQLHPKAEIIISEADHHANFVTWSEIARQYGATLHILPINEQWLIEEQDLIAVLNTNT
HHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHCCEEEEEECCCCEEEECCCEEEEEECCE
VLVALNMVSNVTGTEQPVANLIKLIRQHSHALVLVDAAQAISHLPIDLQRLDADFIAFSA
EEEEEEHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHCCCHHHEEEH
HKLYGPNGLGVLSGKRHALEQLHPLLYGGKMVERVSAQHIRFAELPYRLEAGTPNIAGVI
HHEECCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCEEEECCCCCCCHHH
GFNAVLEWLSQWDLTAAEQHAIALAEQCKMRLKNYPHCQLFLSPQPSSIVCFVFNGIATS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHH
DIATLLAEQNIALRAGEHCAQPYLARLGQHSTLRLSFAPYNQQADVDAFFSALDNALALL
HHHHHHHCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100