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The map label for this gene is nagE [H]

Identifier: 15602741

GI number: 15602741

Start: 1030460

End: 1031917

Strand: Direct

Name: nagE [H]

Synonym: PM0876

Alternate gene names: 15602741

Gene position: 1030460-1031917 (Clockwise)

Preceding gene: 15602738

Following gene: 15602746

Centisome position: 45.65

GC content: 42.73

Gene sequence:

>1458_bases
ATGAGCATTCTAAGTTACTTACAAAGAATTGGGCAAGCATTAATGGTGCCAGTTGCTGCACTACCTGCTGCTGCTATCTT
AATGGGTATTGGTTACTGGATTGACCCGAATGGTTGGGGTGGAGAAAGCCAGCTTGCTGCCTTCTTGATCAAGTCTGGTG
CCGCCATTATTGACAACATGGCAATTCTGTTCGCCGTTGGTGTGGCATTTGGTATGTCTAAAGATAAACATGGTTCAGCC
GCACTGTCGGGCTTAGTGGGCTTTTTAGTTGTCACCACCTTGCTTTCTCCAGGTGCTGTTGCACAATTACAAGGCATTGC
TGCCGATCAAGTCCCTGCCGCATTTGGCAAAATTAACAACCAATTTATTGGGATTTTAGTTGGGGTTATTTCTGCTGAAT
TATATAACCGCTTCCATCAAGTTGAATTACCACCAGCGTTAGCCTTCTTCAGTGGTAAACGTTTAGTGCCTATCGTGACC
TCTGTCGTGATGATCGTTGTGTCTTTCATCCTCATGTACTTATGGCCAGTCATTTTTAATGGTTTAGTTGGTTTTGGTAA
ATCGATCACGGATATTGGCGCAACTGGGGCGGGTATCTATGGTTTCTTAAACCGTCTCTTAATTCCAGTCGGCTTACACC
ACGCTTTAAACTCAGTGTTCTGGTTCAATGTGGCGGGTATCAACGATATTCCTAATTTCTTAGGTGGTGCACAATCTCTT
GCAAATGGTACTGCCGTTCTTGGTGTAACAGGAATGTATCAAGCTGGTTTCTTCCCTGTTATGATGTTCGGTTTACCTGC
CGCCGCATTAGCAATCTATCATAGTGCAAAACCTGGTAAAAAAGCACAAGTTGCCTCATTAATGATTGCCGCATCATTAG
CTTCATTCTTCACAGGGGTAACAGAGCCATTAGAATTTGCGTTCATGTTTGTGGCGCCAGTCTTATACGTCATTCACGCC
TTATTAACTGGTCTTTCTGTCTTTATTGCAGCCTCAATGCAATGGATGGCAGGTTTTGGTTTCAGTGCAGGTTTCGTTGA
TATGGTACTTTCTTCACAAAACCCACTGGCTAAAGATTGGTACATGTTACTCGTACAAGGTCTTGTATTCGGTGTAATTT
ACTATGTTATCTTCCGTGCTGCAATCAAAGCGTTCAATTTAAAAACACCAGGCCGTGAAGAAGAGGAAGTGGAAATCAAT
GTGAAAAAAGACGCCTCTCGTGAAGAACGTGCGGCTAACTTCATCGTTGCATTGGGTGGTAAAGATAACTTCCAAGCTAT
CGATGCTTGTATTACTCGCTTACGTTTAACATTAGTTGATCGTAGCAAAGTGAATGAAGAGCAATTAAAAGCACTAGGTT
CAAAAGGTAATGTCAAATTAGGTGACAATGGTTTACAAGTGATTCTTGGACCTGAAGCTGAGCTAGTAGCGGATGCAATC
AAACAACAAGTCAAGTAA

Upstream 100 bases:

>100_bases
ATTTGCAATAGAAAATTATCAGCATATAGTTAGAGACGCTTGAGAAAACGTTTACTTTTTTCTCAACAAAATCGAGAGAA
AAATATAAAAAGGAGTTCCT

Downstream 100 bases:

>100_bases
CATATTTACTTGAAAACAAGGGCGAAAAATAATAATTTTTCGCCCTTTTCTCTTTTGTAAATACCGTCAAATCCTACGAC
ATCAATTAGCCTGATTTACT

Product: hypothetical protein

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSILSYLQRIGQALMVPVAALPAAAILMGIGYWIDPNGWGGESQLAAFLIKSGAAIIDNMAILFAVGVAFGMSKDKHGSA
ALSGLVGFLVVTTLLSPGAVAQLQGIAADQVPAAFGKINNQFIGILVGVISAELYNRFHQVELPPALAFFSGKRLVPIVT
SVVMIVVSFILMYLWPVIFNGLVGFGKSITDIGATGAGIYGFLNRLLIPVGLHHALNSVFWFNVAGINDIPNFLGGAQSL
ANGTAVLGVTGMYQAGFFPVMMFGLPAAALAIYHSAKPGKKAQVASLMIAASLASFFTGVTEPLEFAFMFVAPVLYVIHA
LLTGLSVFIAASMQWMAGFGFSAGFVDMVLSSQNPLAKDWYMLLVQGLVFGVIYYVIFRAAIKAFNLKTPGREEEEVEIN
VKKDASREERAANFIVALGGKDNFQAIDACITRLRLTLVDRSKVNEEQLKALGSKGNVKLGDNGLQVILGPEAELVADAI
KQQVK

Sequences:

>Translated_485_residues
MSILSYLQRIGQALMVPVAALPAAAILMGIGYWIDPNGWGGESQLAAFLIKSGAAIIDNMAILFAVGVAFGMSKDKHGSA
ALSGLVGFLVVTTLLSPGAVAQLQGIAADQVPAAFGKINNQFIGILVGVISAELYNRFHQVELPPALAFFSGKRLVPIVT
SVVMIVVSFILMYLWPVIFNGLVGFGKSITDIGATGAGIYGFLNRLLIPVGLHHALNSVFWFNVAGINDIPNFLGGAQSL
ANGTAVLGVTGMYQAGFFPVMMFGLPAAALAIYHSAKPGKKAQVASLMIAASLASFFTGVTEPLEFAFMFVAPVLYVIHA
LLTGLSVFIAASMQWMAGFGFSAGFVDMVLSSQNPLAKDWYMLLVQGLVFGVIYYVIFRAAIKAFNLKTPGREEEEVEIN
VKKDASREERAANFIVALGGKDNFQAIDACITRLRLTLVDRSKVNEEQLKALGSKGNVKLGDNGLQVILGPEAELVADAI
KQQVK
>Mature_484_residues
SILSYLQRIGQALMVPVAALPAAAILMGIGYWIDPNGWGGESQLAAFLIKSGAAIIDNMAILFAVGVAFGMSKDKHGSAA
LSGLVGFLVVTTLLSPGAVAQLQGIAADQVPAAFGKINNQFIGILVGVISAELYNRFHQVELPPALAFFSGKRLVPIVTS
VVMIVVSFILMYLWPVIFNGLVGFGKSITDIGATGAGIYGFLNRLLIPVGLHHALNSVFWFNVAGINDIPNFLGGAQSLA
NGTAVLGVTGMYQAGFFPVMMFGLPAAALAIYHSAKPGKKAQVASLMIAASLASFFTGVTEPLEFAFMFVAPVLYVIHAL
LTGLSVFIAASMQWMAGFGFSAGFVDMVLSSQNPLAKDWYMLLVQGLVFGVIYYVIFRAAIKAFNLKTPGREEEEVEINV
KKDASREERAANFIVALGGKDNFQAIDACITRLRLTLVDRSKVNEEQLKALGSKGNVKLGDNGLQVILGPEAELVADAIK
QQVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=499, Percent_Identity=44.0881763527054, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1787343, Length=493, Percent_Identity=36.7139959432049, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787908, Length=530, Percent_Identity=34.1509433962264, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51527; Mature: 51396

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSYLQRIGQALMVPVAALPAAAILMGIGYWIDPNGWGGESQLAAFLIKSGAAIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCHHHHHHH
AILFAVGVAFGMSKDKHGSAALSGLVGFLVVTTLLSPGAVAQLQGIAADQVPAAFGKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCH
QFIGILVGVISAELYNRFHQVELPPALAFFSGKRLVPIVTSVVMIVVSFILMYLWPVIFN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVGFGKSITDIGATGAGIYGFLNRLLIPVGLHHALNSVFWFNVAGINDIPNFLGGAQSL
HHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
ANGTAVLGVTGMYQAGFFPVMMFGLPAAALAIYHSAKPGKKAQVASLMIAASLASFFTGV
CCCCEEEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFAFMFVAPVLYVIHALLTGLSVFIAASMQWMAGFGFSAGFVDMVLSSQNPLAKDW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHH
YMLLVQGLVFGVIYYVIFRAAIKAFNLKTPGREEEEVEINVKKDASREERAANFIVALGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHCCEEEEECC
KDNFQAIDACITRLRLTLVDRSKVNEEQLKALGSKGNVKLGDNGLQVILGPEAELVADAI
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCEEEECCCHHHHHHHH
KQQVK
HHHCC
>Mature Secondary Structure 
SILSYLQRIGQALMVPVAALPAAAILMGIGYWIDPNGWGGESQLAAFLIKSGAAIIDNM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCHHHHHHH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HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]