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The map label for this gene is nagB

Identifier: 15602740

GI number: 15602740

Start: 1029349

End: 1030152

Strand: Reverse

Name: nagB

Synonym: PM0875

Alternate gene names: 15602740

Gene position: 1030152-1029349 (Counterclockwise)

Preceding gene: 15602742

Following gene: 15602739

Centisome position: 45.63

GC content: 38.43

Gene sequence:

>804_bases
ATGCGTTTAATTCCATTACACAATGTTGATCAAGTCGCAAAATGGTCTGCACGTTATATTGTTGATCGTATTAATCAATT
TCAACCAACAGAAGCGCGCCCATTCGTATTAGGTTTGCCTACGGGTGGTACTCCGCTGAAAACCTATGAAGCGCTTATTG
AACTGTATAAAGCTGGTGAGGTGAGCTTTAAACATGTTGTGACCTTCAATATGGATGAGTATGTTGGTTTACCAAAAGAA
CATCCTGAAAGCTACCATTCTTTCATGTATAAAAACTTTTTTGATCATGTTGATATTCAAGAGAAAAATATCAATATCTT
AAATGGTAATACCGAAGATCATGATGCCGAATGTCAGCGTTATGAAGAGAAAATCAAGTCTTATGGCAAAATTCATTTAT
TCATGGGCGGTGTTGGTGTAGATGGTCATATTGCTTTTAATGAACCGGCTTCATCCCTTTCCTCACGTACGCGTATAAAA
ACGCTTACAGAAGATACGCTAATTGCCAACTCACGTTTCTTTGATAACGATGTTAATAAAGTGCCTAAATATGCACTCAC
CATTGGTGTGGGGACGTTACTTGATGCAGAAGAAGTGATGATTCTAGTCACAGGTTATAATAAAGCTCAAGCTTTACAAG
CCGCTGTAGAAGGTAGCATCAATCATTTATGGACCGTCACCGCATTACAAATGCATCGCCGAGCGATTATTGTGTGTGAT
GAACCTGCGACGCAAGAGCTTAAAGTGAAAACAGTTAAATATTTTACTGAATTAGAAGCCTCTGCTATTCGTAGCGTGAA
ATAA

Upstream 100 bases:

>100_bases
TATTGAAAAAAAAATTGATGGCAATATTTCAAAATGGTGTTGGCGATAGGGGAATCCTTGATTTCACAGTAATTTTACAT
ACAAAAAGAGGTAACACATT

Downstream 100 bases:

>100_bases
AAGGAGATAAGCATGTACGCGTTTGTTAATGCCGTAATTTACACTGCAAAAGACGTACTTTATGGTAAAGCGCTTGTTGT
CGATGGCGATAAAATTAGTG

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRLIPLHNVDQVAKWSARYIVDRINQFQPTEARPFVLGLPTGGTPLKTYEALIELYKAGEVSFKHVVTFNMDEYVGLPKE
HPESYHSFMYKNFFDHVDIQEKNINILNGNTEDHDAECQRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTEDTLIANSRFFDNDVNKVPKYALTIGVGTLLDAEEVMILVTGYNKAQALQAAVEGSINHLWTVTALQMHRRAIIVCD
EPATQELKVKTVKYFTELEASAIRSVK

Sequences:

>Translated_267_residues
MRLIPLHNVDQVAKWSARYIVDRINQFQPTEARPFVLGLPTGGTPLKTYEALIELYKAGEVSFKHVVTFNMDEYVGLPKE
HPESYHSFMYKNFFDHVDIQEKNINILNGNTEDHDAECQRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTEDTLIANSRFFDNDVNKVPKYALTIGVGTLLDAEEVMILVTGYNKAQALQAAVEGSINHLWTVTALQMHRRAIIVCD
EPATQELKVKTVKYFTELEASAIRSVK
>Mature_267_residues
MRLIPLHNVDQVAKWSARYIVDRINQFQPTEARPFVLGLPTGGTPLKTYEALIELYKAGEVSFKHVVTFNMDEYVGLPKE
HPESYHSFMYKNFFDHVDIQEKNINILNGNTEDHDAECQRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTEDTLIANSRFFDNDVNKVPKYALTIGVGTLLDAEEVMILVTGYNKAQALQAAVEGSINHLWTVTALQMHRRAIIVCD
EPATQELKVKTVKYFTELEASAIRSVK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=62.015503875969, Blast_Score=343, Evalue=6e-95,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=61.2403100775194, Blast_Score=340, Evalue=7e-94,
Organism=Escherichia coli, GI1786893, Length=266, Percent_Identity=76.3157894736842, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=28.3842794759825, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994958, Length=241, Percent_Identity=24.4813278008299, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=61.2403100775194, Blast_Score=328, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24581960, Length=255, Percent_Identity=61.1764705882353, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI19920764, Length=255, Percent_Identity=61.1764705882353, Blast_Score=346, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_PASMU (Q9CMF4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245812.1
- ProteinModelPortal:   Q9CMF4
- SMR:   Q9CMF4
- PRIDE:   Q9CMF4
- GeneID:   1244222
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0875
- NMPDR:   fig|272843.1.peg.875
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- BioCyc:   PMUL272843:PM0875-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 30208; Mature: 30208

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLHNVDQVAKWSARYIVDRINQFQPTEARPFVLGLPTGGTPLKTYEALIELYKAGE
CEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVGLPKEHPESYHSFMYKNFFDHVDIQEKNINILNGNTEDHDAECQR
CEEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDTLIANSRFFDNDVNK
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHECCCCCEEECCCCCCCCHHH
VPKYALTIGVGTLLDAEEVMILVTGYNKAQALQAAVEGSINHLWTVTALQMHRRAIIVCD
CCCEEEEEECCHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
EPATQELKVKTVKYFTELEASAIRSVK
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLIPLHNVDQVAKWSARYIVDRINQFQPTEARPFVLGLPTGGTPLKTYEALIELYKAGE
CEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVGLPKEHPESYHSFMYKNFFDHVDIQEKNINILNGNTEDHDAECQR
CEEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDTLIANSRFFDNDVNK
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHECCCCCEEECCCCCCCCHHH
VPKYALTIGVGTLLDAEEVMILVTGYNKAQALQAAVEGSINHLWTVTALQMHRRAIIVCD
CCCEEEEEECCHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
EPATQELKVKTVKYFTELEASAIRSVK
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100