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The map label for this gene is ilvI [H]

Identifier: 15602735

GI number: 15602735

Start: 1022734

End: 1024458

Strand: Reverse

Name: ilvI [H]

Synonym: PM0870

Alternate gene names: 15602735

Gene position: 1024458-1022734 (Counterclockwise)

Preceding gene: 15602736

Following gene: 15602734

Centisome position: 45.38

GC content: 40.46

Gene sequence:

>1725_bases
ATGAAAAAGTTATCTGGCGCAGAAATGGTTGTGCAGTCTTTGCGAGATGAAGGCGTTGAGTTTTTATTTGGTTATCCTGG
CGGATCGGTTTTAGATATTTATGATGCGATTCATACCTTGGGGGGCATTGAACATATTCTTGTCCGACATGAACAAGCCG
CTGTGCACATGGCAGATGGTTATGCCAGAGCGACCGGTAAAGTGGGTTGTGTTCTTGTGACATCCGGACCCGGTGCAACT
AATACCATCACTGGTATTGCAACAGCGTATATGGATTCCGTGCCTTTGATTATTTTATCTGGACAGGTGATGTCAGGATT
AATTGGCAGTGATGCTTTTCAAGAATGCGATATGGTGGGTATCTCTCGTCCAGTCGTCAAACACAGTTTTTTAGTGAAAT
CTGCGGCTGAAATTCCTGAAACCATTAAAAAAGCTTTTTATATTGCGTCAACAGGCAGACCGGGACCTGTGGTGATCGAT
TTGCCAAAGGATGTGGTTAATCCTGCACATAAATTTACTTATCATTATCCAGAAAGAGTTGAATTACGTTCTTATAATCC
AACAATACAAGGGCATAAAGGGCAAATTAAAAAAGCATTAAAAGCCTTATTAGTCGCGAAAAAGCCGGTGTTATTTGTTG
GTGGTGGTGTCATCACTGCGAATTGTAGCGACAAATTAACGCAATTTGCACAAACCTTAAATTTGCCGGTCACTTCCTCC
TTAATGGGATTAGGTGCCTATCCAAGCTCAGATAAGCAATTCTTAGGGATGTTAGGCATGCATGGTACTTACGAAGCGAA
TAATGCAATGCATCATAGTGATCTCATTCTTGGTATTGGTGTGCGTTTTGATGATAGAACGACTAATAACTTGGCAAAAT
ATTGTCCACATGCAAAAGTGATTCATATTGATATTGATCCAACGTCTATTTCTAAAAATGTATTGGCATCTATTCCAATT
GTGGGCAGTGCAGAAAAAGTCATTAATGAATTTTTAGCCTTATTAGAGGATGACAATTTATTAAAATCGCAAACTGCCTT
GGATGAATGGTGGCAGCAAATTGATGAATGGAAAGCAGTAAAATGTCTCTCTTTTACACGAGATGATTCCGTCATTAAAC
CACAGCAAGTGATGGAAGTGGTTTATCGATTAACACAAGGTGAGGCTTATGTCGCCTCAGATGTTGGACAACATCAAATG
TTTGCGGCATTACATTATCCGTTTAACCAACCTCGGCGTTGGATTAACTCTGGTGGCTTAGGGACCATGGGCTTTGGACT
ACCAGCCGCACTTGGGGTAAAGCTCGCTTATCCTGACTCAACAGTGGTGTGTGTGACGGGGGATGGCAGTATTCAAATGA
ATATTCAAGAGCTTTCTACTGCACAGCAATATGATATTCCAATTGTGATTATCAGTTTGAATAACCGCTTTTTAGGCATG
GTGAAACAGTGGCAAGATCTGATTTATTCTGGACGCCATTCACAGTCTTATATGAATTCTTTACCTGATTTTGTGAAGCT
TGCTGAGGCTTATGGACATGTGGGTATCCAAATTAATACGCCAGATGAATTAGAGGAAAAACTCACTCAAGCATTTTCGA
TGAAAGATAAATTAGTATTTGTCGATATTAATGTTGATGCGACGGAACATGTTTATCCGATGCAAGTACGTGGTGGTGCA
ATGAATGAAATGATTTTAACAAAACCGGAGAAGGCGAATGCGTAG

Upstream 100 bases:

>100_bases
GGCGGTTTCCAGTAAAAATGAGAACGTGTTAATGCATGACATTATAACAACATCAATTAAAGCAATAAGCACGTAAAGTG
TAAGACATTTAGAGGTGAAA

Downstream 100 bases:

>100_bases
AATTTTATCAGTATTATTAGAAAATGAATCGGGAGCATTATCTCGTGTGGTAGGCTTATTTTCACAACGTGCATTCAATA
TAGAAAGCTTAACTGTTGCC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKKLSGAEMVVQSLRDEGVEFLFGYPGGSVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARATGKVGCVLVTSGPGAT
NTITGIATAYMDSVPLIILSGQVMSGLIGSDAFQECDMVGISRPVVKHSFLVKSAAEIPETIKKAFYIASTGRPGPVVID
LPKDVVNPAHKFTYHYPERVELRSYNPTIQGHKGQIKKALKALLVAKKPVLFVGGGVITANCSDKLTQFAQTLNLPVTSS
LMGLGAYPSSDKQFLGMLGMHGTYEANNAMHHSDLILGIGVRFDDRTTNNLAKYCPHAKVIHIDIDPTSISKNVLASIPI
VGSAEKVINEFLALLEDDNLLKSQTALDEWWQQIDEWKAVKCLSFTRDDSVIKPQQVMEVVYRLTQGEAYVASDVGQHQM
FAALHYPFNQPRRWINSGGLGTMGFGLPAALGVKLAYPDSTVVCVTGDGSIQMNIQELSTAQQYDIPIVIISLNNRFLGM
VKQWQDLIYSGRHSQSYMNSLPDFVKLAEAYGHVGIQINTPDELEEKLTQAFSMKDKLVFVDINVDATEHVYPMQVRGGA
MNEMILTKPEKANA

Sequences:

>Translated_574_residues
MKKLSGAEMVVQSLRDEGVEFLFGYPGGSVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARATGKVGCVLVTSGPGAT
NTITGIATAYMDSVPLIILSGQVMSGLIGSDAFQECDMVGISRPVVKHSFLVKSAAEIPETIKKAFYIASTGRPGPVVID
LPKDVVNPAHKFTYHYPERVELRSYNPTIQGHKGQIKKALKALLVAKKPVLFVGGGVITANCSDKLTQFAQTLNLPVTSS
LMGLGAYPSSDKQFLGMLGMHGTYEANNAMHHSDLILGIGVRFDDRTTNNLAKYCPHAKVIHIDIDPTSISKNVLASIPI
VGSAEKVINEFLALLEDDNLLKSQTALDEWWQQIDEWKAVKCLSFTRDDSVIKPQQVMEVVYRLTQGEAYVASDVGQHQM
FAALHYPFNQPRRWINSGGLGTMGFGLPAALGVKLAYPDSTVVCVTGDGSIQMNIQELSTAQQYDIPIVIISLNNRFLGM
VKQWQDLIYSGRHSQSYMNSLPDFVKLAEAYGHVGIQINTPDELEEKLTQAFSMKDKLVFVDINVDATEHVYPMQVRGGA
MNEMILTKPEKANA
>Mature_574_residues
MKKLSGAEMVVQSLRDEGVEFLFGYPGGSVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARATGKVGCVLVTSGPGAT
NTITGIATAYMDSVPLIILSGQVMSGLIGSDAFQECDMVGISRPVVKHSFLVKSAAEIPETIKKAFYIASTGRPGPVVID
LPKDVVNPAHKFTYHYPERVELRSYNPTIQGHKGQIKKALKALLVAKKPVLFVGGGVITANCSDKLTQFAQTLNLPVTSS
LMGLGAYPSSDKQFLGMLGMHGTYEANNAMHHSDLILGIGVRFDDRTTNNLAKYCPHAKVIHIDIDPTSISKNVLASIPI
VGSAEKVINEFLALLEDDNLLKSQTALDEWWQQIDEWKAVKCLSFTRDDSVIKPQQVMEVVYRLTQGEAYVASDVGQHQM
FAALHYPFNQPRRWINSGGLGTMGFGLPAALGVKLAYPDSTVVCVTGDGSIQMNIQELSTAQQYDIPIVIISLNNRFLGM
VKQWQDLIYSGRHSQSYMNSLPDFVKLAEAYGHVGIQINTPDELEEKLTQAFSMKDKLVFVDINVDATEHVYPMQVRGGA
MNEMILTKPEKANA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=26.7605633802817, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI21361361, Length=507, Percent_Identity=26.232741617357, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=69.4589877835951, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=38.4070796460177, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=33.8827838827839, Blast_Score=321, Evalue=9e-89,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=27.2727272727273, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1788716, Length=569, Percent_Identity=23.5500878734622, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=555, Percent_Identity=27.9279279279279, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=555, Percent_Identity=27.9279279279279, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=523, Percent_Identity=25.0478011472275, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=40.7470288624788, Blast_Score=432, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=25.2525252525253, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=501, Percent_Identity=22.5548902195609, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=582, Percent_Identity=26.2886597938144, Blast_Score=185, Evalue=8e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62727; Mature: 62727

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSGAEMVVQSLRDEGVEFLFGYPGGSVLDIYDAIHTLGGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGCVLVTSGPGATNTITGIATAYMDSVPLIILSGQVMSGLIGSDAFQECDMVG
CEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKSAAEIPETIKKAFYIASTGRPGPVVIDLPKDVVNPAHKFTYHYPERV
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCHHHEEECCCCCE
ELRSYNPTIQGHKGQIKKALKALLVAKKPVLFVGGGVITANCSDKLTQFAQTLNLPVTSS
EEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHCCCHHHH
LMGLGAYPSSDKQFLGMLGMHGTYEANNAMHHSDLILGIGVRFDDRTTNNLAKYCPHAKV
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
IHIDIDPTSISKNVLASIPIVGSAEKVINEFLALLEDDNLLKSQTALDEWWQQIDEWKAV
EEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHE
KCLSFTRDDSVIKPQQVMEVVYRLTQGEAYVASDVGQHQMFAALHYPFNQPRRWINSGGL
EEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHEEECCCCHHHHHHCCCCC
GTMGFGLPAALGVKLAYPDSTVVCVTGDGSIQMNIQELSTAQQYDIPIVIISLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHCCHHHCCCCEEEEEECCHHHHH
VKQWQDLIYSGRHSQSYMNSLPDFVKLAEAYGHVGIQINTPDELEEKLTQAFSMKDKLVF
HHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCEEE
VDINVDATEHVYPMQVRGGAMNEMILTKPEKANA
EEECCCCCCCEEEEEECCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKKLSGAEMVVQSLRDEGVEFLFGYPGGSVLDIYDAIHTLGGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGCVLVTSGPGATNTITGIATAYMDSVPLIILSGQVMSGLIGSDAFQECDMVG
CEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKSAAEIPETIKKAFYIASTGRPGPVVIDLPKDVVNPAHKFTYHYPERV
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCHHHEEECCCCCE
ELRSYNPTIQGHKGQIKKALKALLVAKKPVLFVGGGVITANCSDKLTQFAQTLNLPVTSS
EEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHCCCHHHH
LMGLGAYPSSDKQFLGMLGMHGTYEANNAMHHSDLILGIGVRFDDRTTNNLAKYCPHAKV
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
IHIDIDPTSISKNVLASIPIVGSAEKVINEFLALLEDDNLLKSQTALDEWWQQIDEWKAV
EEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHE
KCLSFTRDDSVIKPQQVMEVVYRLTQGEAYVASDVGQHQMFAALHYPFNQPRRWINSGGL
EEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHEEECCCCHHHHHHCCCCC
GTMGFGLPAALGVKLAYPDSTVVCVTGDGSIQMNIQELSTAQQYDIPIVIISLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHCCHHHCCCCEEEEEECCHHHHH
VKQWQDLIYSGRHSQSYMNSLPDFVKLAEAYGHVGIQINTPDELEEKLTQAFSMKDKLVF
HHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCEEE
VDINVDATEHVYPMQVRGGAMNEMILTKPEKANA
EEECCCCCCCEEEEEECCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]