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The map label for this gene is fruR [H]

Identifier: 15602733

GI number: 15602733

Start: 1021012

End: 1022001

Strand: Reverse

Name: fruR [H]

Synonym: PM0868

Alternate gene names: 15602733

Gene position: 1022001-1021012 (Counterclockwise)

Preceding gene: 15602734

Following gene: 15602731

Centisome position: 45.27

GC content: 39.19

Gene sequence:

>990_bases
GTGAAATTAAAGTTAGTTGAGCTCGCAAAACTTGCAGGAGTATCACGTACAACCGCCAGTTATGTGGTTAATGGGAAGGC
GAAGGCATATCGCGTTAGTGATAAAACCATTGAAAAAGTCGAAAAACTGATTAAACAATATGATTTCAAGCCTAATGCGA
TGGCAGCTGGGCTACGAGCGGGGAAAAGTCGCACTATTGGTTTAATTGTCCCTGATTTTGAAAACGCGAGTTTTGCCAGA
ATTGCAACACATCTAGAAGCTGGATTTCGCCAGAAAGGTTATCAATTAGTCATCGCTTGTTCTAATGATAACCCAGAAAC
AGAAATGCAATGTGCTAAGCATCTTATCCAACGTCAAATTGACGCCCTGATTGTATCCACGACATTACCTGCCGATACTG
ATTTTTATCAACGCCATGCCAATATTCCGATTATTTGCTTCGATCGCTATCTTGATCTCAATAAGATACGTGTTTTGACA
GACGATGAGGGCGACTCATATCGATTAGCAACACATTTAGGGAGACATTGTGAGGGAAAGCGGATGCTTTTTTTAGGCGC
GCTACCTAAGCTAGGGCTCAGTCGAAAACGAGAACTTGGTTTTCGTCAAGCGCTCTCGGATAAGGTGAATCAAGTGGATT
ATCTTTATGCCTCACAGTTTCATAAATTAGCAAGTGCAAAGGCGTTTCATCATTGGTTAGAGAAACATGAGATGCCAGAA
GCTATTTTTGCCACATCATTAACATTGTTACATGGTGTTTTTCAGATTTTGATTAAACGTTATCATCGTATTCCACGTTC
GCTTATCATTGCAACTTTTGGTCATCACGATATGTTAGATCTGTTGGAAAATAAAGTGATTTGTTGTGTACAAAATCACG
AAAAAATTGCACAGACCTTACTGCATCTTGTATTAAGTCGAATGAAATCAAAGAACGTTAAAGTGTCTGATGAAGTAATT
ATTCGAGAAATTATCGAATCACGCATTTAA

Upstream 100 bases:

>100_bases
TTATTTACAAATATTTATTTATTTTTAATAAATATTTACAATAAAGAGATAATCATCATCGTCACCACCTCAATAGTTTT
ATACATTTTAAGGGGGAAAT

Downstream 100 bases:

>100_bases
TGTGATGGTACGTCTTCAAGTGCTACGATAGGGACCGTTGTCGGTGGTGGCAACGGTTCTGGCTCACCTAAAAAATAAGG
TTCCTTTTCCATTAAAATAT

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRAGKSRTIGLIVPDFENASFAR
IATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQIDALIVSTTLPADTDFYQRHANIPIICFDRYLDLNKIRVLT
DDEGDSYRLATHLGRHCEGKRMLFLGALPKLGLSRKRELGFRQALSDKVNQVDYLYASQFHKLASAKAFHHWLEKHEMPE
AIFATSLTLLHGVFQILIKRYHRIPRSLIIATFGHHDMLDLLENKVICCVQNHEKIAQTLLHLVLSRMKSKNVKVSDEVI
IREIIESRI

Sequences:

>Translated_329_residues
MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRAGKSRTIGLIVPDFENASFAR
IATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQIDALIVSTTLPADTDFYQRHANIPIICFDRYLDLNKIRVLT
DDEGDSYRLATHLGRHCEGKRMLFLGALPKLGLSRKRELGFRQALSDKVNQVDYLYASQFHKLASAKAFHHWLEKHEMPE
AIFATSLTLLHGVFQILIKRYHRIPRSLIIATFGHHDMLDLLENKVICCVQNHEKIAQTLLHLVLSRMKSKNVKVSDEVI
IREIIESRI
>Mature_329_residues
MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRAGKSRTIGLIVPDFENASFAR
IATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQIDALIVSTTLPADTDFYQRHANIPIICFDRYLDLNKIRVLT
DDEGDSYRLATHLGRHCEGKRMLFLGALPKLGLSRKRELGFRQALSDKVNQVDYLYASQFHKLASAKAFHHWLEKHEMPE
AIFATSLTLLHGVFQILIKRYHRIPRSLIIATFGHHDMLDLLENKVICCVQNHEKIAQTLLHLVLSRMKSKNVKVSDEVI
IREIIESRI

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=50.4854368932039, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=22.9299363057325, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790369, Length=326, Percent_Identity=24.2331288343558, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787948, Length=314, Percent_Identity=21.3375796178344, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787580, Length=289, Percent_Identity=23.1833910034602, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790715, Length=145, Percent_Identity=28.9655172413793, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788474, Length=270, Percent_Identity=26.2962962962963, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789068, Length=282, Percent_Identity=24.468085106383, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787906, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37459; Mature: 37459

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRA
CCCHHHHHHHHHCCCCCHHHHEECCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GKSRTIGLIVPDFENASFARIATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DALIVSTTLPADTDFYQRHANIPIICFDRYLDLNKIRVLTDDEGDSYRLATHLGRHCEGK
HHEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHCCCCCCC
RMLFLGALPKLGLSRKRELGFRQALSDKVNQVDYLYASQFHKLASAKAFHHWLEKHEMPE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AIFATSLTLLHGVFQILIKRYHRIPRSLIIATFGHHDMLDLLENKVICCVQNHEKIAQTL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHCCCEEEEECCHHHHHHHH
LHLVLSRMKSKNVKVSDEVIIREIIESRI
HHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKLVELAKLAGVSRTTASYVVNGKAKAYRVSDKTIEKVEKLIKQYDFKPNAMAAGLRA
CCCHHHHHHHHHCCCCCHHHHEECCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GKSRTIGLIVPDFENASFARIATHLEAGFRQKGYQLVIACSNDNPETEMQCAKHLIQRQI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DALIVSTTLPADTDFYQRHANIPIICFDRYLDLNKIRVLTDDEGDSYRLATHLGRHCEGK
HHEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHCCCCCCC
RMLFLGALPKLGLSRKRELGFRQALSDKVNQVDYLYASQFHKLASAKAFHHWLEKHEMPE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AIFATSLTLLHGVFQILIKRYHRIPRSLIIATFGHHDMLDLLENKVICCVQNHEKIAQTL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHCCCEEEEECCHHHHHHHH
LHLVLSRMKSKNVKVSDEVIIREIIESRI
HHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]