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The map label for this gene is aroA

Identifier: 15602704

GI number: 15602704

Start: 988954

End: 990276

Strand: Direct

Name: aroA

Synonym: PM0839

Alternate gene names: 15602704

Gene position: 988954-990276 (Clockwise)

Preceding gene: 15602703

Following gene: 15602706

Centisome position: 43.81

GC content: 44.37

Gene sequence:

>1323_bases
GTGATAAAAGATGCGACCGCTATTACTCTCAATCCCATCAGCTATATTGAAGGCGAGGTGCGTTTACCGGGCTCCAAAAG
CTTATCCAATCGCGCACTCTTACTTTCCGCATTAGCTAAAGGAAAAACAACATTAACCAATCTGTTAGATAGTGATGATG
TGCGCCATATGTTAAATGCGTTAAAAGAACTTGGCGTGACTTATCAACTCTCAGAAGACAAATCCGTCTGTGAAATTGAA
GGCTTAGGACGTGCTTTTGAATGGCAAAGTGGCTTAGCTTTATTTTTGGGCAATGCAGGGACGGCGATGCGTCCCTTGAC
TGCCGCGCTTTGTTTATCTACACCGAACAAGGAAGGCAAAAATGAAATAGTCTTGACTGGCGAACCTCGTATGAAAGAAC
GCCCAATACAACATTTAGTTGATGCATTATGTCAAGCTGGCGCAGAAATTCAGTATTTAGAACAAGAAGGTTACCCACCT
ATCGCCATTCGAAATACCGGACTCAAAGGCGGACGAATACAAATTGATGGGTCAGTTTCTTCTCAATTTTTGACCGCACT
TTTAATGGCTGCCCCGATGGCAGAGGCGGATACGGAAATTGAAATCATCGGTGAGCTGGTTTCCAAACCTTACATTGATA
TCACCCTTAAGATGATGCAAACCTTTGGCGTTGAAGTTGAAAACCAAGCCTATCAACGCTTTTTGGTGAAAGGTCATCAG
CAATACCAATCACCACACAGGTTTCTAGTAGAAGGCGATGCCTCTTCTGCTTCTTATTTTCTTGCCGCGGCAGCAATCAA
GGGAAAAGTAAAAGTCACAGGCGTCGGTAAAAATAGCATTCAAGGGGATCGTCTGTTTGCGGATGTGCTAGAAAAAATGG
GGGCGCATATCACTTGGGGCGACGATTTTATTCAAGTGGAAAAAGGCAACCTCAAAGGCATCGATATGGATATGAACCAT
ATTCCCGATGCGGCAATGACCATTGCCACCACAGCGCTTTTTGCAGAAGGTGAAACGGTCATTCGTAATATTTATAACTG
GCGCGTAAAAGAAACTGATCGCTTGACCGCGATGGCGACCGAACTGCGTAAGGTGGGGGCGGAAGTGGAAGAAGGCGAAG
ATTTTATTCGTATCCAGCCATTGAATCTGGCGCAATTTCAACATGCTGAAATTGAAACATACAATGATCACCGCATGGCG
ATGTGCTTTGCTTTAATCGCATTGTCGCAAACGTCGGTCACGATTTTAGACCCGAGCTGTACCGCAAAAACGTTTCCTAC
GTTTTTTGATACTTTTTTACGCTTAACACACGCAGAAAGTTAG

Upstream 100 bases:

>100_bases
AGCATCGGTTTACCAGAAGAAAACCAACGTTTCTTACTCGCATTAAACGAAGTATTAGGGCTTTAAGCCAAACTTATCCA
GTGTATAGGAAATATAAATC

Downstream 100 bases:

>100_bases
CCTACAGATAAGTACTCAGCAAAAAGCGAAGTATTTTATAATACTTCGCTTTTTGAGTGACGAATCGGGTCTGAGATAAT
GCAAGAAAACACCTTAAGAC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNALKELGVTYQLSEDKSVCEIE
GLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNKEGKNEIVLTGEPRMKERPIQHLVDALCQAGAEIQYLEQEGYPP
IAIRNTGLKGGRIQIDGSVSSQFLTALLMAAPMAEADTEIEIIGELVSKPYIDITLKMMQTFGVEVENQAYQRFLVKGHQ
QYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSIQGDRLFADVLEKMGAHITWGDDFIQVEKGNLKGIDMDMNH
IPDAAMTIATTALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLNLAQFQHAEIETYNDHRMA
MCFALIALSQTSVTILDPSCTAKTFPTFFDTFLRLTHAES

Sequences:

>Translated_440_residues
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNALKELGVTYQLSEDKSVCEIE
GLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNKEGKNEIVLTGEPRMKERPIQHLVDALCQAGAEIQYLEQEGYPP
IAIRNTGLKGGRIQIDGSVSSQFLTALLMAAPMAEADTEIEIIGELVSKPYIDITLKMMQTFGVEVENQAYQRFLVKGHQ
QYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSIQGDRLFADVLEKMGAHITWGDDFIQVEKGNLKGIDMDMNH
IPDAAMTIATTALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLNLAQFQHAEIETYNDHRMA
MCFALIALSQTSVTILDPSCTAKTFPTFFDTFLRLTHAES
>Mature_440_residues
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNALKELGVTYQLSEDKSVCEIE
GLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNKEGKNEIVLTGEPRMKERPIQHLVDALCQAGAEIQYLEQEGYPP
IAIRNTGLKGGRIQIDGSVSSQFLTALLMAAPMAEADTEIEIIGELVSKPYIDITLKMMQTFGVEVENQAYQRFLVKGHQ
QYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSIQGDRLFADVLEKMGAHITWGDDFIQVEKGNLKGIDMDMNH
IPDAAMTIATTALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLNLAQFQHAEIETYNDHRMA
MCFALIALSQTSVTILDPSCTAKTFPTFFDTFLRLTHAES

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=433, Percent_Identity=70.2078521939954, Blast_Score=600, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6320332, Length=449, Percent_Identity=40.7572383073497, Blast_Score=272, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_PASMU (Q04570)

Other databases:

- EMBL:   Z14100
- EMBL:   AE004439
- PIR:   S28063
- RefSeq:   NP_245776.1
- ProteinModelPortal:   Q04570
- GeneID:   1244186
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0839
- NMPDR:   fig|272843.1.peg.839
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- BioCyc:   PMUL272843:PM0839-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 48381; Mature: 48381

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNA
CCCCCEEEEECCHHHCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHH
LKELGVTYQLSEDKSVCEIEGLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNKEGK
HHHCCCEEEECCCCCEEEECCCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCC
NEIVLTGEPRMKERPIQHLVDALCQAGAEIQYLEQEGYPPIAIRNTGLKGGRIQIDGSVS
CEEEEECCCCHHHCHHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCHH
SQFLTALLMAAPMAEADTEIEIIGELVSKPYIDITLKMMQTFGVEVENQAYQRFLVKGHQ
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSIQGDRLFADVLEKMGAHITWG
HHCCCCEEEEECCCCCCHHEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEC
DDFIQVEKGNLKGIDMDMNHIPDAAMTIATTALFAEGETVIRNIYNWRVKETDRLTAMAT
CCEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHH
ELRKVGAEVEEGEDFIRIQPLNLAQFQHAEIETYNDHRMAMCFALIALSQTSVTILDPSC
HHHHHCCCCCCCCCEEEEECCCHHHCCCCCCEECCCHHHHHHHHHHHHCCCEEEEECCCC
TAKTFPTFFDTFLRLTHAES
CCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNA
CCCCCEEEEECCHHHCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHH
LKELGVTYQLSEDKSVCEIEGLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNKEGK
HHHCCCEEEECCCCCEEEECCCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCC
NEIVLTGEPRMKERPIQHLVDALCQAGAEIQYLEQEGYPPIAIRNTGLKGGRIQIDGSVS
CEEEEECCCCHHHCHHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCHH
SQFLTALLMAAPMAEADTEIEIIGELVSKPYIDITLKMMQTFGVEVENQAYQRFLVKGHQ
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSIQGDRLFADVLEKMGAHITWG
HHCCCCEEEEECCCCCCHHEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEC
DDFIQVEKGNLKGIDMDMNHIPDAAMTIATTALFAEGETVIRNIYNWRVKETDRLTAMAT
CCEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHH
ELRKVGAEVEEGEDFIRIQPLNLAQFQHAEIETYNDHRMAMCFALIALSQTSVTILDPSC
HHHHHCCCCCCCCCEEEEECCCHHHCCCCCCEECCCHHHHHHHHHHHHCCCEEEEECCCC
TAKTFPTFFDTFLRLTHAES
CCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1474900; 11248100