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The map label for this gene is rimK

Identifier: 15602691

GI number: 15602691

Start: 975707

End: 976648

Strand: Reverse

Name: rimK

Synonym: PM0826

Alternate gene names: 15602691

Gene position: 976648-975707 (Counterclockwise)

Preceding gene: 15602694

Following gene: 15602690

Centisome position: 43.26

GC content: 42.99

Gene sequence:

>942_bases
ATGAAATTATTAATGTTATGTCGTGAGCCACGATTATATAGCTGTCAACGTTTAAAAGAAGCAGCCGAAAATAGTGGACA
CCAAATGGATATTCTTGATCCCAATCGTTGCTTGTTGAAACTTTCTGAAAATTCACCGCACTTTGAGCTTATTTATCAAG
CTACAATGGAGCACGAGCCTAGCCGCTTGTTAAATTACGATGCTGTTATTCCGCGTTTTGGTTCTCAAAGCACCAAAATG
GGCTGTGCAGTTTTACGCCATTTTGAAGCACAAAATATCCCTTGTTTGAATTCTGCCACGGCATTTTTACAGGCTCGCGA
TAAATGGCAAAGCTTACAAATGTTGATGGCACAAGGCATCGCGATACCTCATTCGCTGTTAGCCGGACCCGAATTTCAGG
CACCGAGTACTATGCAATATGTGCAATCGCCGACTATTTTAAAAACATTGTCTGGGGCGCAAGGTATTGGTGTGATTTTG
GCTGAGAAGAGGCAAAGTGCGGTCAGTATTTTAGAAACATTAAAGCAGGCAGACGTGTCAGTGCTCGCACAAGAATTCAT
TGAAGAAGCGAATGGGGCAGATCTACGTTGTTTTGTGATTGGTGAACGAGTTGTCGCCAGTATGCAACGGATAAGTCAGA
ATGGCGAATTTCGCGCCAATTTTCATCGTGGTGGTTTGGCGGAAAAAGTGAGTTTATCTGAAGCGGAAAAAACGATGGCG
GTCAAGGCAACAAAAGCGTTGGGATTAGATGTCGCGGGAGTGGATCTCATTCGTGCTAAACGGGGGACATTAGTCCTTGA
AGTAAACGCCAGTCCGGGTTTAGAGATGATTGAAAAAACCAGCGGTATTGATATTGCATTGCAAATGATCGTACACCTTG
AGAAAAAATATCAGGCGTTGCAAAATGTCACAAAGACAATCGAATTGAGGAGTCATAAATGA

Upstream 100 bases:

>100_bases
TTACCTCATACAAAGAAAAAGAATAAAGTATTTTTCAGCCTGACTTCGCTTAGTGTAATATAATCACTTATTATCTGTTT
TTGTTTCCTCGTGAATAGTT

Downstream 100 bases:

>100_bases
TTATTTATTTACATGGGTTTGATTCAAGTAGCCCGGGCAATCATGAAAAAGTGATGCAACTGAAATTTATTGATGAGGAT
GTCCGTTTTGTTAATTACAG

Product: RimK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLLMLCREPRLYSCQRLKEAAENSGHQMDILDPNRCLLKLSENSPHFELIYQATMEHEPSRLLNYDAVIPRFGSQSTKM
GCAVLRHFEAQNIPCLNSATAFLQARDKWQSLQMLMAQGIAIPHSLLAGPEFQAPSTMQYVQSPTILKTLSGAQGIGVIL
AEKRQSAVSILETLKQADVSVLAQEFIEEANGADLRCFVIGERVVASMQRISQNGEFRANFHRGGLAEKVSLSEAEKTMA
VKATKALGLDVAGVDLIRAKRGTLVLEVNASPGLEMIEKTSGIDIALQMIVHLEKKYQALQNVTKTIELRSHK

Sequences:

>Translated_313_residues
MKLLMLCREPRLYSCQRLKEAAENSGHQMDILDPNRCLLKLSENSPHFELIYQATMEHEPSRLLNYDAVIPRFGSQSTKM
GCAVLRHFEAQNIPCLNSATAFLQARDKWQSLQMLMAQGIAIPHSLLAGPEFQAPSTMQYVQSPTILKTLSGAQGIGVIL
AEKRQSAVSILETLKQADVSVLAQEFIEEANGADLRCFVIGERVVASMQRISQNGEFRANFHRGGLAEKVSLSEAEKTMA
VKATKALGLDVAGVDLIRAKRGTLVLEVNASPGLEMIEKTSGIDIALQMIVHLEKKYQALQNVTKTIELRSHK
>Mature_313_residues
MKLLMLCREPRLYSCQRLKEAAENSGHQMDILDPNRCLLKLSENSPHFELIYQATMEHEPSRLLNYDAVIPRFGSQSTKM
GCAVLRHFEAQNIPCLNSATAFLQARDKWQSLQMLMAQGIAIPHSLLAGPEFQAPSTMQYVQSPTILKTLSGAQGIGVIL
AEKRQSAVSILETLKQADVSVLAQEFIEEANGADLRCFVIGERVVASMQRISQNGEFRANFHRGGLAEKVSLSEAEKTMA
VKATKALGLDVAGVDLIRAKRGTLVLEVNASPGLEMIEKTSGIDIALQMIVHLEKKYQALQNVTKTIELRSHK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=208, Percent_Identity=27.8846153846154, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI134254462, Length=207, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787076, Length=296, Percent_Identity=43.9189189189189, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_PASMU (Q9CMJ8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245763.1
- ProteinModelPortal:   Q9CMJ8
- GeneID:   1244173
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0826
- NMPDR:   fig|272843.1.peg.826
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- ProtClustDB:   CLSK870260
- BioCyc:   PMUL272843:PM0826-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 34600; Mature: 34600

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLMLCREPRLYSCQRLKEAAENSGHQMDILDPNRCLLKLSENSPHFELIYQATMEHEP
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHCCCH
SRLLNYDAVIPRFGSQSTKMGCAVLRHFEAQNIPCLNSATAFLQARDKWQSLQMLMAQGI
HHHHCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
AIPHSLLAGPEFQAPSTMQYVQSPTILKTLSGAQGIGVILAEKRQSAVSILETLKQADVS
CCCHHHHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
VLAQEFIEEANGADLRCFVIGERVVASMQRISQNGEFRANFHRGGLAEKVSLSEAEKTMA
HHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
VKATKALGLDVAGVDLIRAKRGTLVLEVNASPGLEMIEKTSGIDIALQMIVHLEKKYQAL
HHHHHHHCCCCCCHHHHEECCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
QNVTKTIELRSHK
HHHHHHHHCCCCC
>Mature Secondary Structure
MKLLMLCREPRLYSCQRLKEAAENSGHQMDILDPNRCLLKLSENSPHFELIYQATMEHEP
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHCCCH
SRLLNYDAVIPRFGSQSTKMGCAVLRHFEAQNIPCLNSATAFLQARDKWQSLQMLMAQGI
HHHHCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
AIPHSLLAGPEFQAPSTMQYVQSPTILKTLSGAQGIGVILAEKRQSAVSILETLKQADVS
CCCHHHHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
VLAQEFIEEANGADLRCFVIGERVVASMQRISQNGEFRANFHRGGLAEKVSLSEAEKTMA
HHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
VKATKALGLDVAGVDLIRAKRGTLVLEVNASPGLEMIEKTSGIDIALQMIVHLEKKYQAL
HHHHHHHCCCCCCHHHHEECCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
QNVTKTIELRSHK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100