Click here to switch to the map view.

The map label for this gene is holC [H]

Identifier: 15602687

GI number: 15602687

Start: 972357

End: 972785

Strand: Reverse

Name: holC [H]

Synonym: PM0822

Alternate gene names: 15602687

Gene position: 972785-972357 (Counterclockwise)

Preceding gene: 15602690

Following gene: 15602686

Centisome position: 43.09

GC content: 42.89

Gene sequence:

>429_bases
ATGTCTAAACACGCGCAATTTTATTTGTTAAGTGAGCAAGCACAAATGGAAAAAATGGCTTGTGATTTTGCCGCATCCGC
GTGGCGTCTTGGTAAGCGCGTGTTAATTGCTTGCGAAACTGAACAACAAGCGTTGGATATTGATGAAGCCTTATGGGCAA
GAGAGGCAGATGAGTTTGTGCCGCATAATTTATCAGGTGAAGTGACGACGTATGCTACACCGATTGAGGTGAGCTGGAAA
GGTAAGCGTAATGCGCAACGCCGAGATCTCTTGATTAATTTACAGGTAGAGGTACCAGATTATGTCAGCAGTTTTACACA
GGTGATTGATTTTGTGCCAGTGGAAGAGCATTTAAAAGTGCTAGCCAGAGAGCGTTATAAACAATTACGACAACTTGGCT
GGCAATTATCAACAGAGCAAATCAAGTAA

Upstream 100 bases:

>100_bases
ACAAAAGAAAATCCTAAATCTGCGAATTAGTATTTTATCTTCTGTGACTTTTTCGATAAAATCATCAAGTTATACCTTTG
ACTCAGGAGCTAAGTGATCG

Downstream 100 bases:

>100_bases
GGGGAGATGATGAAATTAGCTGAAGCCCTCATGCAACGTGCAGATGCCCAACGCCGTTTGGCGCAACTGAATCAGCGTTT
ACAGCAAAATGCAAAATATC

Product: DNA polymerase III subunit chi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MSKHAQFYLLSEQAQMEKMACDFAASAWRLGKRVLIACETEQQALDIDEALWAREADEFVPHNLSGEVTTYATPIEVSWK
GKRNAQRRDLLINLQVEVPDYVSSFTQVIDFVPVEEHLKVLARERYKQLRQLGWQLSTEQIK

Sequences:

>Translated_142_residues
MSKHAQFYLLSEQAQMEKMACDFAASAWRLGKRVLIACETEQQALDIDEALWAREADEFVPHNLSGEVTTYATPIEVSWK
GKRNAQRRDLLINLQVEVPDYVSSFTQVIDFVPVEEHLKVLARERYKQLRQLGWQLSTEQIK
>Mature_141_residues
SKHAQFYLLSEQAQMEKMACDFAASAWRLGKRVLIACETEQQALDIDEALWAREADEFVPHNLSGEVTTYATPIEVSWKG
KRNAQRRDLLINLQVEVPDYVSSFTQVIDFVPVEEHLKVLARERYKQLRQLGWQLSTEQIK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=142, Percent_Identity=51.4084507042254, Blast_Score=155, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007459 [H]

Pfam domain/function: PF04364 DNA_pol3_chi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16469; Mature: 16338

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHAQFYLLSEQAQMEKMACDFAASAWRLGKRVLIACETEQQALDIDEALWAREADEFV
CCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCHHHHHHHHCHHHHC
PHNLSGEVTTYATPIEVSWKGKRNAQRRDLLINLQVEVPDYVSSFTQVIDFVPVEEHLKV
CCCCCCCEEEEECCEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHH
LARERYKQLRQLGWQLSTEQIK
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKHAQFYLLSEQAQMEKMACDFAASAWRLGKRVLIACETEQQALDIDEALWAREADEFV
CCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCHHHHHHHHCHHHHC
PHNLSGEVTTYATPIEVSWKGKRNAQRRDLLINLQVEVPDYVSSFTQVIDFVPVEEHLKV
CCCCCCCEEEEECCEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHH
LARERYKQLRQLGWQLSTEQIK
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 7959067 [H]