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The map label for this gene is argG

Identifier: 15602678

GI number: 15602678

Start: 961842

End: 963179

Strand: Reverse

Name: argG

Synonym: PM0813

Alternate gene names: 15602678

Gene position: 963179-961842 (Counterclockwise)

Preceding gene: 15602679

Following gene: 15602677

Centisome position: 42.67

GC content: 42.0

Gene sequence:

>1338_bases
ATGTCAAACACAATTTTACAGCAGCTTCCAAAAGGTCAAAAAGTCGGTATCGCGTTTTCAGGTGGTTTAGATACCAGTGC
AGCATTGTTATGGATGCGTCAAAAAGGGGCTGTTCCTTATGCCTATACCGCGAACTTAGGTCAACCAGATGAAGAGGATT
ACAATGCAATTCCGCGTAAGGCGATGGAATATGGCGCGGAAAATGCCCGCTTAATTGATTGCCGTGCACAGCTTGCTCAC
GAAGGGATTGCGGCTATTCAAAGCGGTGCCTTCCATATTTCAACAGGTGGCGTAACGTATTTTAATACCACCCCATTGGG
TCGTGCAGTAACGGGAACGATGTTAGTGTCGGCGATGCGTGAAGATGATGTGAATATCTGGGGTGATGGCAGTACCTTCA
AAGGTAATGATATTGAGCGTTTCTATCGTTATGGTTTATTAACTAATCCCAATCTTAAAATTTATAAGCCATGGTTAGAT
AATCAATTTATCGAAGAATTAGGCGGTCGTCATGAAATGTCAGAGTTCTTGATTGCGAATGGCTTTGAATACAAAATGTC
AGTGGAAAAAGCCTATTCAACAGATTCAAATATGCTAGGTGCCACCCATGAAGCCAAAGATCTTGAATTTTTAAATACGG
GTATTCGTATTGTAAAACCGATTATGGGCGTGGCATTCTGGCGTGATGATGTTGAAGTGAAAGCTGAAGAAGTCACTGTG
CGCTTTGAAGAGGGGATTCCTGTGGCGTTAAATGGTAAAACATTCAGCGATCATGTTGAATTATTCCTCGAAGCAAACCG
TATTGGTGGGCGCCATGGTTTAGGTATGTCAGACCAAATTGAAAACCGAATTATTGAAGCCAAATCGCGTGGTATTTATG
AGGCACCGGGGATGGCGTTGTTACATATTGCCTATGAGCGCTTAGTGAGTGGTATCCATAATGAAGATACGATTGAACAA
TACCGTATTAACGGCTTACGTTTAGGTCGTTTACTCTACCAAGGTCGTTGGTTTGATCCACAAGCCTTAATGTTACGTGA
AACGGCACAACGCTGGGTTGCGCGTGCGATTACCGGTGAGGTGACTTTCGAATTACGCCGTGGTAATGATTATTCCATTT
TGAATACGGAATCACCAAATTTAACTTATATGCCAGAGCGTTTAAGTATGGAAAAAGTGGAAAATGCGCCATTTGATCCT
GTTGATCGTATTGGTCAATTAACGATGCGCAACTTAGATATCGTAGATACGCGGGATAAGTTAGGTATTTACACGCAAAC
AGGCTTACTCAGTATTTCAAAAGATTCTTTCTTGCCACAACTAAATAAGAAAGGTTAA

Upstream 100 bases:

>100_bases
AATGAGGAAAAGAGCGGTGGACTTTCCGATGGGTTTTTGAAATAATAAAGTGATTATTTAATCACTTTTTTTGCGTAATT
ATTTTAAATAAAGGTCAAGT

Downstream 100 bases:

>100_bases
TGCCTTTTTTGTGCTGAGAAAGTTTTAAAAAATAGACCGCACTTTGCCTAATTGGCGCTGAAAAGTGCGGTATTTTTTTA
CAGACTTTCTGAACTACCTT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSNTILQQLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYNAIPRKAMEYGAENARLIDCRAQLAH
EGIAAIQSGAFHISTGGVTYFNTTPLGRAVTGTMLVSAMREDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLD
NQFIEELGGRHEMSEFLIANGFEYKMSVEKAYSTDSNMLGATHEAKDLEFLNTGIRIVKPIMGVAFWRDDVEVKAEEVTV
RFEEGIPVALNGKTFSDHVELFLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVSGIHNEDTIEQ
YRINGLRLGRLLYQGRWFDPQALMLRETAQRWVARAITGEVTFELRRGNDYSILNTESPNLTYMPERLSMEKVENAPFDP
VDRIGQLTMRNLDIVDTRDKLGIYTQTGLLSISKDSFLPQLNKKG

Sequences:

>Translated_445_residues
MSNTILQQLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYNAIPRKAMEYGAENARLIDCRAQLAH
EGIAAIQSGAFHISTGGVTYFNTTPLGRAVTGTMLVSAMREDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLD
NQFIEELGGRHEMSEFLIANGFEYKMSVEKAYSTDSNMLGATHEAKDLEFLNTGIRIVKPIMGVAFWRDDVEVKAEEVTV
RFEEGIPVALNGKTFSDHVELFLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVSGIHNEDTIEQ
YRINGLRLGRLLYQGRWFDPQALMLRETAQRWVARAITGEVTFELRRGNDYSILNTESPNLTYMPERLSMEKVENAPFDP
VDRIGQLTMRNLDIVDTRDKLGIYTQTGLLSISKDSFLPQLNKKG
>Mature_444_residues
SNTILQQLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYNAIPRKAMEYGAENARLIDCRAQLAHE
GIAAIQSGAFHISTGGVTYFNTTPLGRAVTGTMLVSAMREDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDN
QFIEELGGRHEMSEFLIANGFEYKMSVEKAYSTDSNMLGATHEAKDLEFLNTGIRIVKPIMGVAFWRDDVEVKAEEVTVR
FEEGIPVALNGKTFSDHVELFLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVSGIHNEDTIEQY
RINGLRLGRLLYQGRWFDPQALMLRETAQRWVARAITGEVTFELRRGNDYSILNTESPNLTYMPERLSMEKVENAPFDPV
DRIGQLTMRNLDIVDTRDKLGIYTQTGLLSISKDSFLPQLNKKG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=28.992628992629, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=28.992628992629, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1789563, Length=438, Percent_Identity=81.2785388127854, Blast_Score=740, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=421, Percent_Identity=28.2660332541568, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358151, Length=396, Percent_Identity=29.040404040404, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PASMU (P57877)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245750.1
- ProteinModelPortal:   P57877
- SMR:   P57877
- GeneID:   1244160
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0813
- NMPDR:   fig|272843.1.peg.813
- HOGENOM:   HBG289560
- OMA:   GGRKEMS
- ProtClustDB:   PRK05370
- BioCyc:   PMUL272843:PM0813-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00581
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 49993; Mature: 49862

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 44-44 BINDING 100-100 BINDING 130-130 BINDING 132-132 BINDING 132-132 BINDING 136-136 BINDING 136-136 BINDING 137-137 BINDING 137-137 BINDING 140-140 BINDING 193-193 BINDING 195-195 BINDING 202-202 BINDING 204-204 BINDING 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTILQQLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYNAIPRK
CCCCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCHHHHHHHHH
AMEYGAENARLIDCRAQLAHEGIAAIQSGAFHISTGGVTYFNTTPLGRAVTGTMLVSAMR
HHHCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHH
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDNQFIEELGGRHEMSEFLIAN
CCCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEECCCCCHHHHHHHCCHHHHHHHHHHC
GFEYKMSVEKAYSTDSNMLGATHEAKDLEFLNTGIRIVKPIMGVAFWRDDVEVKAEEVTV
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEE
RFEEGIPVALNGKTFSDHVELFLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCCHHHHHEEEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVSGIHNEDTIEQYRINGLRLGRLLYQGRWFDPQALMLRETAQRWVARAITGE
HHHHHHHHHHCCCCCCHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCE
VTFELRRGNDYSILNTESPNLTYMPERLSMEKVENAPFDPVDRIGQLTMRNLDIVDTRDK
EEEEEECCCCEEEEECCCCCEEECCHHHCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCC
LGIYTQTGLLSISKDSFLPQLNKKG
CCEEEECCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SNTILQQLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYNAIPRK
CCCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCHHHHHHHHH
AMEYGAENARLIDCRAQLAHEGIAAIQSGAFHISTGGVTYFNTTPLGRAVTGTMLVSAMR
HHHCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHH
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDNQFIEELGGRHEMSEFLIAN
CCCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEECCCCCHHHHHHHCCHHHHHHHHHHC
GFEYKMSVEKAYSTDSNMLGATHEAKDLEFLNTGIRIVKPIMGVAFWRDDVEVKAEEVTV
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEE
RFEEGIPVALNGKTFSDHVELFLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCCHHHHHEEEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVSGIHNEDTIEQYRINGLRLGRLLYQGRWFDPQALMLRETAQRWVARAITGE
HHHHHHHHHHCCCCCCHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCE
VTFELRRGNDYSILNTESPNLTYMPERLSMEKVENAPFDPVDRIGQLTMRNLDIVDTRDK
EEEEEECCCCEEEEECCCCCEEECCHHHCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCC
LGIYTQTGLLSISKDSFLPQLNKKG
CCEEEECCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100