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The map label for this gene is tpl

Identifier: 15602676

GI number: 15602676

Start: 959657

End: 961033

Strand: Reverse

Name: tpl

Synonym: PM0811

Alternate gene names: 15602676

Gene position: 961033-959657 (Counterclockwise)

Preceding gene: 15602677

Following gene: 15602675

Centisome position: 42.57

GC content: 40.74

Gene sequence:

>1377_bases
ATGAGAAACTATCCTGCAGAACCTTATAAAGTCAAAGCTGTTGAACCGATTGCAATGACGACTCGTGAGCAACGTGAAGC
CTACATGAAAAAAGCGGGCTACAATACGTTTTTACTCAATTCAGAAGAAGTGTATATTGATTTATTAACCGATAGTGGTA
CCTCGGCCATGAGTGATAAGCAATGGGCGGGTCTTATGATCGGTGATGAAGCTTATGCAGGTAGCCGTAACTTCATGCAT
TTACAAGACGTGGTCAGAGAATATTATGGTTTCAAATATGTCGTACCAACTCACCAAGGTCGTGGTGCAGAAAACTTACT
TTCAACCATTATGATCAAACCGGGCGATTATGTACCGGGTAATATGTATTTTACTACAACACGCGCGCACCAAGAGCGTA
ATGGGGCGACTTTCGTTGATATTATTATTGATGAAGCCCATGATTCACAAATTGATTTACCTTTCAAAGGCAATGTCGAT
GTGAAGAAATTACAAAAACTGATTGATGAAGTGGGTGCGGATAAAATTCCTTACATCTGTTTAGCAGTTACCGTAAACTT
GGCAGGTGGACAACCTGTGAGTATGGCAAACATGCGTGAAGTAAAAGCATTATGTAGCAAACATGGTATTAAAGTGATGT
TTGATGCCACACGTTGTGTAGAAAATGCGTACTTCATTAAAGAGCGTGAAGCAGAGTATAAAGATGCAACCATCAAAGAT
ATTCTAAAAGAAATGATGAGTTATGCAGATGGTTGTACCATGAGTGGTAAAAAAGACTGTCTTGTGAATATTGGTGGTTT
CTTATGTATTAATGATGATGATCTTTATCAACAAGCTTGTGAACTCGTGGTGCTCTTTGAAGGGATGCCATCTTATGGTG
GACTAGCTGGTCGTGATATGGAAGCAATGGCAATTGGTATTACGGAATCTGTCGATTTCCACTATATCCAGCACCGTGTT
GCACAATGTTACTACCTCGCGGATAAATTAGAAGCGGCGGGTGTGCCGATTGTGAAACCAGTGGGTGGTCATGCGGTATT
CTTAGATGCGAAGAAATTCCTTCCACATATTCCACAAGAACAATTCCCAGCACAGATGCTTGCTGCGCAAATTTATATTG
AGGGTGGCGTACGCTCAATGGAACGTGGTATTGTATCGGCGGGTCGTGATAAGAAAACCGGTGCAAACCATACGCCTAAA
TTAGAATTAGTGCGTTTAACTATCCCACGTCGTGTTTATACTTATGCGCATTTAGATCATGTTGCTGACACCATTATTAA
ACTCTTTAAACACAGAGATGACATTAAAGGTCTTGATATGGTGTATGAGCCTAAATTATTACGTTTCTTTACGGCGCGTT
TTGAACCGAAAGCGTAA

Upstream 100 bases:

>100_bases
TAAGGGTTGTTAGTGGTAAAAGTTGTTTAATAACAAGATCTTAGTTTTCTTTTCGGTAAATGTTATTAATATTATTGATG
ATCATTTTAGGAGAAAGATT

Downstream 100 bases:

>100_bases
TTTCTCATTAGGGCATTGCTGAAAAGCAATGTCCTACTTATTCAATATGTCTGCTGTTTTCATTATTATGGGATAGACAA
AAAGGAGATATATTATGAAT

Product: tyrosine phenol-lyase

Products: NA

Alternate protein names: Beta-tyrosinase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRNYPAEPYKVKAVEPIAMTTREQREAYMKKAGYNTFLLNSEEVYIDLLTDSGTSAMSDKQWAGLMIGDEAYAGSRNFMH
LQDVVREYYGFKYVVPTHQGRGAENLLSTIMIKPGDYVPGNMYFTTTRAHQERNGATFVDIIIDEAHDSQIDLPFKGNVD
VKKLQKLIDEVGADKIPYICLAVTVNLAGGQPVSMANMREVKALCSKHGIKVMFDATRCVENAYFIKEREAEYKDATIKD
ILKEMMSYADGCTMSGKKDCLVNIGGFLCINDDDLYQQACELVVLFEGMPSYGGLAGRDMEAMAIGITESVDFHYIQHRV
AQCYYLADKLEAAGVPIVKPVGGHAVFLDAKKFLPHIPQEQFPAQMLAAQIYIEGGVRSMERGIVSAGRDKKTGANHTPK
LELVRLTIPRRVYTYAHLDHVADTIIKLFKHRDDIKGLDMVYEPKLLRFFTARFEPKA

Sequences:

>Translated_458_residues
MRNYPAEPYKVKAVEPIAMTTREQREAYMKKAGYNTFLLNSEEVYIDLLTDSGTSAMSDKQWAGLMIGDEAYAGSRNFMH
LQDVVREYYGFKYVVPTHQGRGAENLLSTIMIKPGDYVPGNMYFTTTRAHQERNGATFVDIIIDEAHDSQIDLPFKGNVD
VKKLQKLIDEVGADKIPYICLAVTVNLAGGQPVSMANMREVKALCSKHGIKVMFDATRCVENAYFIKEREAEYKDATIKD
ILKEMMSYADGCTMSGKKDCLVNIGGFLCINDDDLYQQACELVVLFEGMPSYGGLAGRDMEAMAIGITESVDFHYIQHRV
AQCYYLADKLEAAGVPIVKPVGGHAVFLDAKKFLPHIPQEQFPAQMLAAQIYIEGGVRSMERGIVSAGRDKKTGANHTPK
LELVRLTIPRRVYTYAHLDHVADTIIKLFKHRDDIKGLDMVYEPKLLRFFTARFEPKA
>Mature_458_residues
MRNYPAEPYKVKAVEPIAMTTREQREAYMKKAGYNTFLLNSEEVYIDLLTDSGTSAMSDKQWAGLMIGDEAYAGSRNFMH
LQDVVREYYGFKYVVPTHQGRGAENLLSTIMIKPGDYVPGNMYFTTTRAHQERNGATFVDIIIDEAHDSQIDLPFKGNVD
VKKLQKLIDEVGADKIPYICLAVTVNLAGGQPVSMANMREVKALCSKHGIKVMFDATRCVENAYFIKEREAEYKDATIKD
ILKEMMSYADGCTMSGKKDCLVNIGGFLCINDDDLYQQACELVVLFEGMPSYGGLAGRDMEAMAIGITESVDFHYIQHRV
AQCYYLADKLEAAGVPIVKPVGGHAVFLDAKKFLPHIPQEQFPAQMLAAQIYIEGGVRSMERGIVSAGRDKKTGANHTPK
LELVRLTIPRRVYTYAHLDHVADTIIKLFKHRDDIKGLDMVYEPKLLRFFTARFEPKA

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family

Homologues:

Organism=Escherichia coli, GI87082323, Length=462, Percent_Identity=45.4545454545455, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPL_PASMU (Q9CMK9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245748.1
- ProteinModelPortal:   Q9CMK9
- SMR:   Q9CMK9
- PRIDE:   Q9CMK9
- GeneID:   1244158
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0811
- NMPDR:   fig|272843.1.peg.811
- HOGENOM:   HBG297784
- OMA:   EAGYNTF
- ProtClustDB:   PRK13237
- BioCyc:   PMUL272843:PM0811-MONOMER
- BRENDA:   4.1.99.2
- HAMAP:   MF_00543
- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176
- InterPro:   IPR013441
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF001386
- TIGRFAMs:   TIGR02618

Pfam domain/function: PF01212 Beta_elim_lyase; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.99.2

Molecular weight: Translated: 51442; Mature: 51442

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNYPAEPYKVKAVEPIAMTTREQREAYMKKAGYNTFLLNSEEVYIDLLTDSGTSAMSDK
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCCCCCC
QWAGLMIGDEAYAGSRNFMHLQDVVREYYGFKYVVPTHQGRGAENLLSTIMIKPGDYVPG
CCCCEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHEEECCCCCCCC
NMYFTTTRAHQERNGATFVDIIIDEAHDSQIDLPFKGNVDVKKLQKLIDEVGADKIPYIC
CEEEEEEHHHHCCCCCEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEE
LAVTVNLAGGQPVSMANMREVKALCSKHGIKVMFDATRCVENAYFIKEREAEYKDATIKD
EEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHEEECCCCCCCCCHHHH
ILKEMMSYADGCTMSGKKDCLVNIGGFLCINDDDLYQQACELVVLFEGMPSYGGLAGRDM
HHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCHHHHHHHCEEEEECCCCCCCCCCCCCC
EAMAIGITESVDFHYIQHRVAQCYYLADKLEAAGVPIVKPVGGHAVFLDAKKFLPHIPQE
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECHHHCCCCCCH
QFPAQMLAAQIYIEGGVRSMERGIVSAGRDKKTGANHTPKLELVRLTIPRRVYTYAHLDH
HHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
VADTIIKLFKHRDDIKGLDMVYEPKLLRFFTARFEPKA
HHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MRNYPAEPYKVKAVEPIAMTTREQREAYMKKAGYNTFLLNSEEVYIDLLTDSGTSAMSDK
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCCCCCC
QWAGLMIGDEAYAGSRNFMHLQDVVREYYGFKYVVPTHQGRGAENLLSTIMIKPGDYVPG
CCCCEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHEEECCCCCCCC
NMYFTTTRAHQERNGATFVDIIIDEAHDSQIDLPFKGNVDVKKLQKLIDEVGADKIPYIC
CEEEEEEHHHHCCCCCEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEE
LAVTVNLAGGQPVSMANMREVKALCSKHGIKVMFDATRCVENAYFIKEREAEYKDATIKD
EEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHEEECCCCCCCCCHHHH
ILKEMMSYADGCTMSGKKDCLVNIGGFLCINDDDLYQQACELVVLFEGMPSYGGLAGRDM
HHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCHHHHHHHCEEEEECCCCCCCCCCCCCC
EAMAIGITESVDFHYIQHRVAQCYYLADKLEAAGVPIVKPVGGHAVFLDAKKFLPHIPQE
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECHHHCCCCCCH
QFPAQMLAAQIYIEGGVRSMERGIVSAGRDKKTGANHTPKLELVRLTIPRRVYTYAHLDH
HHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
VADTIIKLFKHRDDIKGLDMVYEPKLLRFFTARFEPKA
HHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100