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The map label for this gene is potE [H]

Identifier: 15602672

GI number: 15602672

Start: 954764

End: 956077

Strand: Direct

Name: potE [H]

Synonym: PM0807

Alternate gene names: 15602672

Gene position: 954764-956077 (Clockwise)

Preceding gene: 15602671

Following gene: 15602673

Centisome position: 42.29

GC content: 42.47

Gene sequence:

>1314_bases
ATGAGTGCAAGAAATAATAAAATTGGTGTTGTTCAACTCACTATTCTTACTATGGTTAACATGATGGGCTCAGGCATCAT
TATGTTACCCACAAAATTGGCAGAAATTGGTACCATTTCAATCTTATCGTGGTTAGTAACTGCCGTTGGTTCTACTGCAC
TGGCTTATGCCTTCGCACAATGTGGTATGTTTAGCAAAAAATCAGGTGGTATGGGTGGCTATGCCGAATATGCTTTCGGT
AAAGCAGGGAATTTCATGGCGAACTATACTTATGGTGTTTCATTGGTCATTGCTAACACAGCCATTGCGATTTCCGCAGT
AGGTTATGGTTCTGAATTACTTGAAGTAAGCCTCTCCCCTGTTGCGATCGCAGGCTGGACAATTATCACTTTATGGCTCG
CGACGTCCCTTAACTTTGGGGGAGCTCGTATTACAGGTAATATTAGTTCATTTACCATTTGGGGTGTCATTATTCCGGTG
GTTGGTTTATGCATTATTGGCTGGAAATGGTTTGATGCCGACATGTATATCAATGCTTGGAACCCACATCATGTGCCGAC
GTTTGAAGCAATTGGGGTGTCTATTTCCATGACATTATGGGCATTCCTTGGCTTAGAATCTGCCTGTGCAAATACCGATG
TGGTCGAAAATCCAGAACGTAACGTACCCATTGCGGTACTCGGCGGCACCTTAGGCGCAGCCGCGATCTATATCGTGTCA
ACCAATGTGATTGCCGGTATTGTACCGAATATGGATTTGGCTAATTCAACCGCGCCTTTCGGATTAGCTTTTGCTCATAT
GTTTAATGAAACAACGGGTAAAATTGTGATGGGCTTGATGGTACTCTCTTGCTTTGGTTCACTCCTCGGCTGGCAGTTCA
CCATTGCTCAAGTCTTCAGAAGCTCTGCTGAAGAAGGCTATTTCCCTGCCTTTTTCAAGAAAGTAACAAGCAAAGATGCA
CCAATCATCGGCATGGTGACAATTACAGTGATTCAAAGCTTGTTATCCTTAATGACGATCAGTCCATCCTTAAACAAACA
ATTTAATGTATTAGTGGATCTTGCCGTCGTCACGAACGTTATCCCTTATATTCTTTCGATGGCTGCATTAATGGTGTTAC
AAAAAGTGGAAAAAGTCCCGGCACAGAAAGCAAAAATCACGACATTTGTCGCCTTTATTGGCTCCGTGTACAGTATTTAT
GCACTCTATGCGGCAGGACAAGAACCGATGTTATATGGTTCAATTGCCACCTTTATTGGTTGGACACTATATGGTTTTGT
GTCAGATAAATTTGATATAAAAGCCAAAGTCTAG

Upstream 100 bases:

>100_bases
ATGGACGTATTCGTGCTTATGGCTACGTATTAACTGAAAACTAAATCATTAATTAAAAAAGTACAGTATGCATTTACTGT
ACTTTTATGAGGATACAATT

Downstream 100 bases:

>100_bases
TTCCGCAGATAAGCTATTCTCTCAAAGTGCGATTTATTTCTTAAATCGCACTTTTTTATAGCAATTCAATCTATCAAAGC
AATTTCTCCTAGCCTTTTAA

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSARNNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISILSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYAFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELLEVSLSPVAIAGWTIITLWLATSLNFGGARITGNISSFTIWGVIIPV
VGLCIIGWKWFDADMYINAWNPHHVPTFEAIGVSISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAAIYIVS
TNVIAGIVPNMDLANSTAPFGLAFAHMFNETTGKIVMGLMVLSCFGSLLGWQFTIAQVFRSSAEEGYFPAFFKKVTSKDA
PIIGMVTITVIQSLLSLMTISPSLNKQFNVLVDLAVVTNVIPYILSMAALMVLQKVEKVPAQKAKITTFVAFIGSVYSIY
ALYAAGQEPMLYGSIATFIGWTLYGFVSDKFDIKAKV

Sequences:

>Translated_437_residues
MSARNNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISILSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYAFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELLEVSLSPVAIAGWTIITLWLATSLNFGGARITGNISSFTIWGVIIPV
VGLCIIGWKWFDADMYINAWNPHHVPTFEAIGVSISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAAIYIVS
TNVIAGIVPNMDLANSTAPFGLAFAHMFNETTGKIVMGLMVLSCFGSLLGWQFTIAQVFRSSAEEGYFPAFFKKVTSKDA
PIIGMVTITVIQSLLSLMTISPSLNKQFNVLVDLAVVTNVIPYILSMAALMVLQKVEKVPAQKAKITTFVAFIGSVYSIY
ALYAAGQEPMLYGSIATFIGWTLYGFVSDKFDIKAKV
>Mature_436_residues
SARNNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISILSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYAFGK
AGNFMANYTYGVSLVIANTAIAISAVGYGSELLEVSLSPVAIAGWTIITLWLATSLNFGGARITGNISSFTIWGVIIPVV
GLCIIGWKWFDADMYINAWNPHHVPTFEAIGVSISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAAIYIVST
NVIAGIVPNMDLANSTAPFGLAFAHMFNETTGKIVMGLMVLSCFGSLLGWQFTIAQVFRSSAEEGYFPAFFKKVTSKDAP
IIGMVTITVIQSLLSLMTISPSLNKQFNVLVDLAVVTNVIPYILSMAALMVLQKVEKVPAQKAKITTFVAFIGSVYSIYA
LYAAGQEPMLYGSIATFIGWTLYGFVSDKFDIKAKV

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=435, Percent_Identity=77.0114942528736, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=412, Percent_Identity=33.252427184466, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1790575, Length=399, Percent_Identity=33.5839598997494, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787890, Length=426, Percent_Identity=26.056338028169, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17540018, Length=329, Percent_Identity=26.7477203647416, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550968, Length=343, Percent_Identity=25.0728862973761, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921172, Length=343, Percent_Identity=25.0728862973761, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46591; Mature: 46460

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARNNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISILSWLVTAVGSTALAYAFAQ
CCCCCCCEEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELLEVSLSP
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEEEHHEEEEEECCCCCHHHHEECCC
VAIAGWTIITLWLATSLNFGGARITGNISSFTIWGVIIPVVGLCIIGWKWFDADMYINAW
EEEHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCEEEECC
NPHHVPTFEAIGVSISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAAIYIVS
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHEEEEEE
TNVIAGIVPNMDLANSTAPFGLAFAHMFNETTGKIVMGLMVLSCFGSLLGWQFTIAQVFR
CCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKVTSKDAPIIGMVTITVIQSLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYILSMAALMVLQKVEKVPAQKAKITTFVAFIGSVYSIYALYAAGQEPMLYGSIATFIG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
WTLYGFVSDKFDIKAKV
HHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SARNNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISILSWLVTAVGSTALAYAFAQ
CCCCCCEEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELLEVSLSP
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEEEHHEEEEEECCCCCHHHHEECCC
VAIAGWTIITLWLATSLNFGGARITGNISSFTIWGVIIPVVGLCIIGWKWFDADMYINAW
EEEHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCEEEECC
NPHHVPTFEAIGVSISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAAIYIVS
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHEEEEEE
TNVIAGIVPNMDLANSTAPFGLAFAHMFNETTGKIVMGLMVLSCFGSLLGWQFTIAQVFR
CCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKVTSKDAPIIGMVTITVIQSLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYILSMAALMVLQKVEKVPAQKAKITTFVAFIGSVYSIYALYAAGQEPMLYGSIATFIG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
WTLYGFVSDKFDIKAKV
HHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]