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The map label for this gene is pyrF

Identifier: 15602662

GI number: 15602662

Start: 941246

End: 941947

Strand: Reverse

Name: pyrF

Synonym: PM0797

Alternate gene names: 15602662

Gene position: 941947-941246 (Counterclockwise)

Preceding gene: 15602663

Following gene: 15602661

Centisome position: 41.73

GC content: 40.17

Gene sequence:

>702_bases
ATGACGAGTAAAATCATTGTTGCACTAGATTATGAAAAAGAAGAAGAGGCATTGTGTTTAGTTGATCAAATTGATCCGAG
CTTATGTCGTTTGAAAGTCGGTAAAGAAATGTTTACTACATTAGGGACGAAATTTGTCAAAGCATTACATGATCGCAACT
TTGATGTGTTTTTAGATTTAAAATTTCATGATATTCCAAATACTGTAGCAAGAGCGGTGCGTTCTGCGGCAGATTTAGGG
GTTTGGATGGTTGATTTACATGCCAGCGGTGGTTTGCGTATGATGGAGGAGGCGAAGAAAATTCTTGAACCTTATGGTAA
AGACGCGCCTCTTTTAATTAGTGTGACGGTATTAACCAGTATGGAAGATCTAGATTTATTGCAAATTGGCATTAATGCTT
CTCCGATGGAACAAGTAATCCGTTTAGCCAATCTAACACAGCGTGCTGGATTAGATGGCGTAGTTTGTTCTCCGCAAGAA
GTGGAAATTTTACGCGCTAACTGTGGTAAAGATTTTAAACTGATTACGCCGGGAATTCGCCCAATCGGCAGTGATTTTGG
TGATCAGCGTCGCGTAATGACACCAGCGGGGGCAATTCAAGCAGGTTCTGACTATTTAGTAATTGGTCGTCCAATTACAC
AAGCAGATAACCCAGCAGAAGTGCTCAAATCAATCAATGCTTCTTTACCTGTGAATAGATAA

Upstream 100 bases:

>100_bases
CAAACTAACCTGGTGTTGTCCATCTTGCCGACATTGGGAAACAATCAAACCGATAGAAGATTAAGCAAACATAATTAATT
GAACACAGAGAGGGAAAATT

Downstream 100 bases:

>100_bases
CAATGACAGATACATTAGTCTATTCGACAGAAACCGGTCGAATTAAAACAGAAAAACCTCAACCAACACGTCCTAAAGGT
GATGGGATTGTGCGTATCCA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTSKIIVALDYEKEEEALCLVDQIDPSLCRLKVGKEMFTTLGTKFVKALHDRNFDVFLDLKFHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEEAKKILEPYGKDAPLLISVTVLTSMEDLDLLQIGINASPMEQVIRLANLTQRAGLDGVVCSPQE
VEILRANCGKDFKLITPGIRPIGSDFGDQRRVMTPAGAIQAGSDYLVIGRPITQADNPAEVLKSINASLPVNR

Sequences:

>Translated_233_residues
MTSKIIVALDYEKEEEALCLVDQIDPSLCRLKVGKEMFTTLGTKFVKALHDRNFDVFLDLKFHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEEAKKILEPYGKDAPLLISVTVLTSMEDLDLLQIGINASPMEQVIRLANLTQRAGLDGVVCSPQE
VEILRANCGKDFKLITPGIRPIGSDFGDQRRVMTPAGAIQAGSDYLVIGRPITQADNPAEVLKSINASLPVNR
>Mature_232_residues
TSKIIVALDYEKEEEALCLVDQIDPSLCRLKVGKEMFTTLGTKFVKALHDRNFDVFLDLKFHDIPNTVARAVRSAADLGV
WMVDLHASGGLRMMEEAKKILEPYGKDAPLLISVTVLTSMEDLDLLQIGINASPMEQVIRLANLTQRAGLDGVVCSPQEV
EILRANCGKDFKLITPGIRPIGSDFGDQRRVMTPAGAIQAGSDYLVIGRPITQADNPAEVLKSINASLPVNR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=227, Percent_Identity=63.4361233480176, Blast_Score=293, Evalue=5e-81,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PASMU (Q9CMM1)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245734.1
- ProteinModelPortal:   Q9CMM1
- SMR:   Q9CMM1
- GeneID:   1244144
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0797
- NMPDR:   fig|272843.1.peg.797
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   PMUL272843:PM0797-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25502; Mature: 25371

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 119-119 BINDING 180-180 BINDING 189-189 BINDING 209-209 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKIIVALDYEKEEEALCLVDQIDPSLCRLKVGKEMFTTLGTKFVKALHDRNFDVFLDL
CCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KFHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEEAKKILEPYGKDAPLLISVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHC
MEDLDLLQIGINASPMEQVIRLANLTQRAGLDGVVCSPQEVEILRANCGKDFKLITPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHEECCCCCCEEEECCCCC
PIGSDFGDQRRVMTPAGAIQAGSDYLVIGRPITQADNPAEVLKSINASLPVNR
CCCCCCCCCCEEECCCCCEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSKIIVALDYEKEEEALCLVDQIDPSLCRLKVGKEMFTTLGTKFVKALHDRNFDVFLDL
CCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KFHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEEAKKILEPYGKDAPLLISVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHC
MEDLDLLQIGINASPMEQVIRLANLTQRAGLDGVVCSPQEVEILRANCGKDFKLITPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHEECCCCCCEEEECCCCC
PIGSDFGDQRRVMTPAGAIQAGSDYLVIGRPITQADNPAEVLKSINASLPVNR
CCCCCCCCCCEEECCCCCEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100