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The map label for this gene is pntA [H]

Identifier: 15602618

GI number: 15602618

Start: 886516

End: 888045

Strand: Reverse

Name: pntA [H]

Synonym: PM0753

Alternate gene names: 15602618

Gene position: 888045-886516 (Counterclockwise)

Preceding gene: 15602621

Following gene: 15602617

Centisome position: 39.34

GC content: 44.97

Gene sequence:

>1530_bases
ATGTTAATTGGTGTACCTAGAGAACTGCTTGATAATGAAAGTCGTGTGGCGGCAACGCCAAAAACGGTACAACAAATCTT
AAAGCTGGGCTTTGAGGTCATTGTTGAACATCATGCCGGTTTTAAAGCGAGTTTTGAAGATCAAGCATTTGCAGAAACAG
GCGCCAAAATTGGCTCAGCGCAGGAAGTTTGGCAGTCAGATATCATTTTTAAAGTGAATGCGCCAACGGATAGCGAAATC
GCACTGATAAAAGAAGGGGCGACTTTGGTGAGCTTTATTTGGCCGGCACAAAATCCAGACTTAATGGAAAAATTGTCAGC
GAAGAAAATTAATGTATTAGCCATGGATGCGGTACCACGTATTTCACGAGCACAATCGTTAGATGCGTTAAGTTCGATGG
CAAATATTGCAGGGTATCGTGCGGTAGTGGAAGCGGCGCATGAATTTGGCAGTTTTTTCACGGGGCAGATTACCGCAGCA
GGTAAAGTGCCACCGGCGAAAGTGCTTGTGATTGGTGCTGGGGTAGCTGGCTTAGCAGCGATTGGTGCAGCAAACAGTTT
AGGTGCGATCGTACGTGCCTTTGACTCGCGTCCTGAAGTGAAAGAACAAGTGGAAAGTATGGGCGCAAGTTTCCTTGAAA
TTGATTTCCAAGAAGAAGGTGGCAGTGGCGATGGCTATGCGAAAGTCATGTCAGAAGAATTTAATCGCCGTGCGCTAGCA
CTTTATGCGGAACAAGCCAAGGAAGTTGATATTATTATCACTACCGCTTTAATTCCGGGTAAACCCGCGCCACGTTTGAT
TAGCAAAGAAATGGTAGCATCCATGAAACCGGGTTCGGTGATTGTGGATTTAGCCGCAGCGACAGGCGGTAACTGTGAGT
TGTCAAAAGCAGGCGAAGTCGTAGTGACCGATAATCAAGTGAAAATCATTGGTTACACCGATTTACCAAGCCGCTTACCA
ACACAGTCCTCTCAATTGTATGGCACCAACCTTGTGAACTTGCTTAAATTGTTATGTAAAGAAAAAGACGGCAAGATCAG
TATTGATTTCGATGATGTAGTATTACGCGGTGTAACGGTGATTCGTGACGGTGAGGTGACTTGGCCTGCACCACCTATCC
AAGTGTCTGCCCAGCCACAAAAAGTGAAAGCCACACTGGTAGAGAAAAAAGAAGTAAAAGCGGTCGATCCGCGTAAAAAA
TACGGTGCGATGGCAGTGGCAGCGTTGTTGTTTATGGCGTTGGCATCTGTGGCACCTGCTGCATTCTTATCGCACTTCAC
CGTGTTCGTATTAGCCTGTGTAGTCGGCTATTATGTGGTGTGGAACGTTAGCCATGCGTTGCATACACCGTTAATGTCAG
TCACAAATGCTATTTCAGGCATTATTATTGTCGGCGCGTTGTTACAAATTGCGCAAGGCAATTTCTTTATTAGTATTCTT
GCATTTATTGCAATTTTAGTCGCAAGTATCAATATCTTTGGGGGCTTTAAAGTGACCCAACGGATGCTTGCTATGTTTAG
AAAAGATTAA

Upstream 100 bases:

>100_bases
ACAATAAATGGTACATTGAAACGGTTTTGACTTATTGTCATCTTTTAGGGGAGTAACAGGGTTACTCAAGATTATCATCA
ATCAATTTGAAGGAAACATT

Downstream 100 bases:

>100_bases
GGAGTAAGCCATGTCTGAAGGTTTAGTACAAGCGGCTTATATTGTCGCAGCATTACTTTTCATTATGAGCTTAGCAGGTC
TGTCTAAACATGAAACTGCA

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLIGVPRELLDNESRVAATPKTVQQILKLGFEVIVEHHAGFKASFEDQAFAETGAKIGSAQEVWQSDIIFKVNAPTDSEI
ALIKEGATLVSFIWPAQNPDLMEKLSAKKINVLAMDAVPRISRAQSLDALSSMANIAGYRAVVEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVESMGASFLEIDFQEEGGSGDGYAKVMSEEFNRRALA
LYAEQAKEVDIIITTALIPGKPAPRLISKEMVASMKPGSVIVDLAAATGGNCELSKAGEVVVTDNQVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGKISIDFDDVVLRGVTVIRDGEVTWPAPPIQVSAQPQKVKATLVEKKEVKAVDPRKK
YGAMAVAALLFMALASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMSVTNAISGIIIVGALLQIAQGNFFISIL
AFIAILVASINIFGGFKVTQRMLAMFRKD

Sequences:

>Translated_509_residues
MLIGVPRELLDNESRVAATPKTVQQILKLGFEVIVEHHAGFKASFEDQAFAETGAKIGSAQEVWQSDIIFKVNAPTDSEI
ALIKEGATLVSFIWPAQNPDLMEKLSAKKINVLAMDAVPRISRAQSLDALSSMANIAGYRAVVEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVESMGASFLEIDFQEEGGSGDGYAKVMSEEFNRRALA
LYAEQAKEVDIIITTALIPGKPAPRLISKEMVASMKPGSVIVDLAAATGGNCELSKAGEVVVTDNQVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGKISIDFDDVVLRGVTVIRDGEVTWPAPPIQVSAQPQKVKATLVEKKEVKAVDPRKK
YGAMAVAALLFMALASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMSVTNAISGIIIVGALLQIAQGNFFISIL
AFIAILVASINIFGGFKVTQRMLAMFRKD
>Mature_509_residues
MLIGVPRELLDNESRVAATPKTVQQILKLGFEVIVEHHAGFKASFEDQAFAETGAKIGSAQEVWQSDIIFKVNAPTDSEI
ALIKEGATLVSFIWPAQNPDLMEKLSAKKINVLAMDAVPRISRAQSLDALSSMANIAGYRAVVEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVESMGASFLEIDFQEEGGSGDGYAKVMSEEFNRRALA
LYAEQAKEVDIIITTALIPGKPAPRLISKEMVASMKPGSVIVDLAAATGGNCELSKAGEVVVTDNQVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGKISIDFDDVVLRGVTVIRDGEVTWPAPPIQVSAQPQKVKATLVEKKEVKAVDPRKK
YGAMAVAALLFMALASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMSVTNAISGIIIVGALLQIAQGNFFISIL
AFIAILVASINIFGGFKVTQRMLAMFRKD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=533, Percent_Identity=50.281425891182, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI122939153, Length=533, Percent_Identity=50.281425891182, Blast_Score=478, Evalue=1e-135,
Organism=Escherichia coli, GI1787887, Length=511, Percent_Identity=74.9510763209393, Blast_Score=774, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=517, Percent_Identity=49.7098646034816, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR016040
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54300; Mature: 54300

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPRELLDNESRVAATPKTVQQILKLGFEVIVEHHAGFKASFEDQAFAETGAKIGSA
CCCCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
QEVWQSDIIFKVNAPTDSEIALIKEGATLVSFIWPAQNPDLMEKLSAKKINVLAMDAVPR
HHHHCCCCEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAVVEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVESMGASFLEIDFQEEGGSGDGYAKVMSEEFNRRALA
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHH
LYAEQAKEVDIIITTALIPGKPAPRLISKEMVASMKPGSVIVDLAAATGGNCELSKAGEV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCE
VVTDNQVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLCKEKDGKISIDFDDVVLRGVTV
EEECCEEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCEEE
IRDGEVTWPAPPIQVSAQPQKVKATLVEKKEVKAVDPRKKYGAMAVAALLFMALASVAPA
EECCCEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFLSHFTVFVLACVVGYYVVWNVSHALHTPLMSVTNAISGIIIVGALLQIAQGNFFISIL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AFIAILVASINIFGGFKVTQRMLAMFRKD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIGVPRELLDNESRVAATPKTVQQILKLGFEVIVEHHAGFKASFEDQAFAETGAKIGSA
CCCCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
QEVWQSDIIFKVNAPTDSEIALIKEGATLVSFIWPAQNPDLMEKLSAKKINVLAMDAVPR
HHHHCCCCEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAVVEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVESMGASFLEIDFQEEGGSGDGYAKVMSEEFNRRALA
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHH
LYAEQAKEVDIIITTALIPGKPAPRLISKEMVASMKPGSVIVDLAAATGGNCELSKAGEV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCE
VVTDNQVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLCKEKDGKISIDFDDVVLRGVTV
EEECCEEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCEEE
IRDGEVTWPAPPIQVSAQPQKVKATLVEKKEVKAVDPRKKYGAMAVAALLFMALASVAPA
EECCCEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFLSHFTVFVLACVVGYYVVWNVSHALHTPLMSVTNAISGIIIVGALLQIAQGNFFISIL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AFIAILVASINIFGGFKVTQRMLAMFRKD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]