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The map label for this gene is bioB

Identifier: 15602244

GI number: 15602244

Start: 447583

End: 448593

Strand: Direct

Name: bioB

Synonym: PM0379

Alternate gene names: 15602244

Gene position: 447583-448593 (Clockwise)

Preceding gene: 15602243

Following gene: 15602253

Centisome position: 19.83

GC content: 44.51

Gene sequence:

>1011_bases
ATGACAACGGCAACGGCATTTGAGATTCGTACACTGACTCCTCACCCGACCTTAGAATATTGGTCTGTGTGTAAAATTGA
AGCCCTGTTTGAGACCCCATTTTTAGATTTGGTTTATCGGGCTGCGCAAGTGCACCGAGAAAACTTTAATCCTAAAGCCA
TTCAATTATCCACTTTAATGTCGATTAAAACGGGGGGATGTCCAGAGGATTGTGGCTACTGTCCGCAGTCAGCACGTTAT
CATACTGGAGTAGAAAAGCAGCAATTACTCGATGTGGAAGAAATTGTTGAAAAAGCCAAAATTGCCAAAGCACGTGGTGC
AGGGCGCTTTTGTATGGGGGCTGCATGGCGTGGACCGAAACCGAAAGACATTGAAAAAGTTACCGCAATCATTAAAGCGG
TGAAAGAACTGGGCTTAGAAACCTGTGGTACCTTTGGTTTATTGCAAGATGGGATGGCAGAAGATTTAAAAGAAGCGGGT
TTGGATTATTATAACCATAATCTCGATACAGCCCCAGAACACTACGGTAATGTGATTGGTACCCGTCAATTTGATGATCG
TATTAATACGTTAGGTAAAGTGCGTAAAGCCGGCTTAAAAGTGTGCTGTGGCGGGATTATTGGCATGAATGAAACCCGTA
AAGAAAGAGCAGGATTAATTGCTAGCTTAGCTAATTTAGACCCGCAACCCGAATCGGTGCCGATTAATCAATTAGTGAAA
GTGGAAGGTACCCCTTTAGCCGATGCGGCAGAATTAGACTGGACAGAATTTGTGCGCACTATTGCGGTGGCGCGTATTAC
TATGCCGAAAAGCTATGTACGTTTATCAGCAGGGCGTCAAGGCATGTCGGAAGAAATGCAAGCCATGTGCTTTATGGCTG
GCGCGAATTCAATCTTCTATGGCGATAAATTACTCGTGACGGGTAACCCAGAAGAAGACGGCGATCAGCGCTTAATGGCG
AAATTAGATTTAGAACCAGAAACAGAAGAGAATCGTTACCGTGCGGAATAA

Upstream 100 bases:

>100_bases
CATGGTAGGATGAGTGAGTCCACCATTTTGAAGGATAATTTTAACGAAAAACAGACCGCACTTTAGGCGGCTTATTAATT
AGATAAGGAAAGAGAAAAAT

Downstream 100 bases:

>100_bases
GTGCGGTAGCGCTTTTTGCTTTTTTTCGTGATGTAACAAAAAAGTCCACCTTGTTTGGTGGACTTTTTGTTTAAATCAGA
TTACTGACAAAGATAACTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTTATAFEIRTLTPHPTLEYWSVCKIEALFETPFLDLVYRAAQVHRENFNPKAIQLSTLMSIKTGGCPEDCGYCPQSARY
HTGVEKQQLLDVEEIVEKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTAIIKAVKELGLETCGTFGLLQDGMAEDLKEAG
LDYYNHNLDTAPEHYGNVIGTRQFDDRINTLGKVRKAGLKVCCGGIIGMNETRKERAGLIASLANLDPQPESVPINQLVK
VEGTPLADAAELDWTEFVRTIAVARITMPKSYVRLSAGRQGMSEEMQAMCFMAGANSIFYGDKLLVTGNPEEDGDQRLMA
KLDLEPETEENRYRAE

Sequences:

>Translated_336_residues
MTTATAFEIRTLTPHPTLEYWSVCKIEALFETPFLDLVYRAAQVHRENFNPKAIQLSTLMSIKTGGCPEDCGYCPQSARY
HTGVEKQQLLDVEEIVEKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTAIIKAVKELGLETCGTFGLLQDGMAEDLKEAG
LDYYNHNLDTAPEHYGNVIGTRQFDDRINTLGKVRKAGLKVCCGGIIGMNETRKERAGLIASLANLDPQPESVPINQLVK
VEGTPLADAAELDWTEFVRTIAVARITMPKSYVRLSAGRQGMSEEMQAMCFMAGANSIFYGDKLLVTGNPEEDGDQRLMA
KLDLEPETEENRYRAE
>Mature_335_residues
TTATAFEIRTLTPHPTLEYWSVCKIEALFETPFLDLVYRAAQVHRENFNPKAIQLSTLMSIKTGGCPEDCGYCPQSARYH
TGVEKQQLLDVEEIVEKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTAIIKAVKELGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYGNVIGTRQFDDRINTLGKVRKAGLKVCCGGIIGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAAELDWTEFVRTIAVARITMPKSYVRLSAGRQGMSEEMQAMCFMAGANSIFYGDKLLVTGNPEEDGDQRLMAK
LDLEPETEENRYRAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=60.2605863192182, Blast_Score=407, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=44.5859872611465, Blast_Score=307, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PASMU (Q9CNP8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245316.1
- HSSP:   P12996
- ProteinModelPortal:   Q9CNP8
- SMR:   Q9CNP8
- GeneID:   1243726
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0379
- NMPDR:   fig|272843.1.peg.379
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2299228
- BioCyc:   PMUL272843:PM0379-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37172; Mature: 37041

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATAFEIRTLTPHPTLEYWSVCKIEALFETPFLDLVYRAAQVHRENFNPKAIQLSTLM
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
SIKTGGCPEDCGYCPQSARYHTGVEKQQLLDVEEIVEKAKIAKARGAGRFCMGAAWRGPK
HHCCCCCCHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCC
PKDIEKVTAIIKAVKELGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYGNVIG
CCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCC
TRQFDDRINTLGKVRKAGLKVCCGGIIGMNETRKERAGLIASLANLDPQPESVPINQLVK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEE
VEGTPLADAAELDWTEFVRTIAVARITMPKSYVRLSAGRQGMSEEMQAMCFMAGANSIFY
ECCCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEE
GDKLLVTGNPEEDGDQRLMAKLDLEPETEENRYRAE
CCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTATAFEIRTLTPHPTLEYWSVCKIEALFETPFLDLVYRAAQVHRENFNPKAIQLSTLM
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
SIKTGGCPEDCGYCPQSARYHTGVEKQQLLDVEEIVEKAKIAKARGAGRFCMGAAWRGPK
HHCCCCCCHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCC
PKDIEKVTAIIKAVKELGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYGNVIG
CCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCC
TRQFDDRINTLGKVRKAGLKVCCGGIIGMNETRKERAGLIASLANLDPQPESVPINQLVK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEE
VEGTPLADAAELDWTEFVRTIAVARITMPKSYVRLSAGRQGMSEEMQAMCFMAGANSIFY
ECCCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEE
GDKLLVTGNPEEDGDQRLMAKLDLEPETEENRYRAE
CCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100