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The map label for this gene is thiP [H]

Identifier: 15602242

GI number: 15602242

Start: 445268

End: 446878

Strand: Direct

Name: thiP [H]

Synonym: PM0377

Alternate gene names: 15602242

Gene position: 445268-446878 (Clockwise)

Preceding gene: 15602241

Following gene: 15602243

Centisome position: 19.72

GC content: 41.71

Gene sequence:

>1611_bases
ATGTTTAAGCGATTTCGTGCATTCACATACCGTCCCGCCAGTTATCTTGGCGGGATGTTGGTGATTGTTTTTCTGATCGC
TTTTTATGCGTTCGCCTTAGGGGCGGTTTTTTCACTCCCTTTTGTGCGCAGTTGGACAGCGTTGTTGAGTGATCAGTATT
TACAACACGTGATCATCTTTAGCTTTTGGCAAGCTTTTCTGTCGGCGGTACTTGCGGTCCTCTTTGGTGTCATTGTAGCA
CGAGCCTTTTTTTATCAACCGTTTGTGGGCAAGAAACTGATCCTCAAATTATTTTCACTGACTTTTGTGTTACCTGCCTT
AGTGGTGATTTTTGGTTTATTAGGCGTGTATGGCGCTTCTGGCTGGTTAGCGATGTTAAGCCAGTTTTTCGCTTGGGATT
GGACCCCTAATATTTACGGCTTAACAGGTATTTTACTGGCGCATCTTTTTTTTAATGTCCCATTAGCTTGTCGCCTGTTT
TTACAAGGTTTGCAAGCAATTCCTGTGCAACAACGTCAGCTCGCGGCACAACTCAATTTACGTGCTTGGCATTTTATACG
TCTGATTGAGTGGCCCTATTTACGCCAGCAATTGTTACCTGCATTTACTTTGATTTTCATGCTGTGTTTTACCAGTTTTG
CGATTGTGCTCACTTTAGGTGGCGGACCGAAATATACCACGTTGGAAGTGGCTATCTATCAAGCGATTTTATTTGAGTTT
GATGTACCGAAAGCCGGCTTATTTGCGTTATTACAATTTGTTTTTTGTTTTCTGTTATTCACGCTGAGTAGCTTTTTTTC
TCCAGCCCCCGCCACGACATTACACAGTCAACCTACTTGGTTTGCGCCACAATCGTATTGGGTTAAATTATGGCAACGTA
TGATCATTGTGTGTGCGACAGTATTTATCTTATTACCGCTACTCAATACGCTAGTTTCTGCTTTGCTTTCGCCTCAGTTT
TTTACCTTGTGGTTACAACCTCAATTATGGAAAGCATTAGGTTACTCGCTCACCATTGCCCCCACTTCTGCATTGCTCGC
TTTAGTACTGTCTTTTGCCGTATTATTGCTTGCTAGAGAATTACATTGGCGACATTATCGCAGCTTATCCCATGTGATTT
TAAATATCGGTGCGACCATTTTAGCCATTCCAACGTTAGTGTTAGCTATTGGTTTATTCATTTTATTACGTGAGATCGAT
TTTTCTCCATACCATCTTTTTGGGGTTGTGGTATGCTGTAACGCGTTAGCTGCAATGCCTTTTGTGTTGCGTATTTTGGC
TTTACCGATGCATAACAACATGATTTATTATGAAAAATTATGCCAATCACTTAACCTGCGTGGTTGGCAACGTTTTCGAT
TGATTGAATGGCACAAGCTTCGTGCGCCAATGAAATATGCCTTTGCACTGGCTTGTGCGTTATCATTAGGCGATTTCACC
GCAATCGCGTTATTTGGTCAGGCTGACTTCACATCGTTACCGCATTTGTTGTATCAACAATTGGGGCATTATCGTAGTCA
GGAAGCAGCAGTAACAGCGTTTATTTTATTGGTTTTTTGTTTGAGTGTTTTTATGATTATTGAACGACATCAGGAACCGC
GTGATGATTAA

Upstream 100 bases:

>100_bases
GAACACGAAAGTGCTCGATACCTCAAAAGTAAATGCCGAACAAGTCAAAAAATGGATTGCTGTTTGGCAAACGACCCTAA
CCCAATAATTGTTTGCCTTG

Downstream 100 bases:

>100_bases
TTTAAACGGTGTTCAGTTTTCCTATAATACCTTTACTTTTGAGCTGGATTTGCAGATTCCTGCTCAACAAAAAGTTGCTA
TTATTGGCGCCAGTGGCGCA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MFKRFRAFTYRPASYLGGMLVIVFLIAFYAFALGAVFSLPFVRSWTALLSDQYLQHVIIFSFWQAFLSAVLAVLFGVIVA
RAFFYQPFVGKKLILKLFSLTFVLPALVVIFGLLGVYGASGWLAMLSQFFAWDWTPNIYGLTGILLAHLFFNVPLACRLF
LQGLQAIPVQQRQLAAQLNLRAWHFIRLIEWPYLRQQLLPAFTLIFMLCFTSFAIVLTLGGGPKYTTLEVAIYQAILFEF
DVPKAGLFALLQFVFCFLLFTLSSFFSPAPATTLHSQPTWFAPQSYWVKLWQRMIIVCATVFILLPLLNTLVSALLSPQF
FTLWLQPQLWKALGYSLTIAPTSALLALVLSFAVLLLARELHWRHYRSLSHVILNIGATILAIPTLVLAIGLFILLREID
FSPYHLFGVVVCCNALAAMPFVLRILALPMHNNMIYYEKLCQSLNLRGWQRFRLIEWHKLRAPMKYAFALACALSLGDFT
AIALFGQADFTSLPHLLYQQLGHYRSQEAAVTAFILLVFCLSVFMIIERHQEPRDD

Sequences:

>Translated_536_residues
MFKRFRAFTYRPASYLGGMLVIVFLIAFYAFALGAVFSLPFVRSWTALLSDQYLQHVIIFSFWQAFLSAVLAVLFGVIVA
RAFFYQPFVGKKLILKLFSLTFVLPALVVIFGLLGVYGASGWLAMLSQFFAWDWTPNIYGLTGILLAHLFFNVPLACRLF
LQGLQAIPVQQRQLAAQLNLRAWHFIRLIEWPYLRQQLLPAFTLIFMLCFTSFAIVLTLGGGPKYTTLEVAIYQAILFEF
DVPKAGLFALLQFVFCFLLFTLSSFFSPAPATTLHSQPTWFAPQSYWVKLWQRMIIVCATVFILLPLLNTLVSALLSPQF
FTLWLQPQLWKALGYSLTIAPTSALLALVLSFAVLLLARELHWRHYRSLSHVILNIGATILAIPTLVLAIGLFILLREID
FSPYHLFGVVVCCNALAAMPFVLRILALPMHNNMIYYEKLCQSLNLRGWQRFRLIEWHKLRAPMKYAFALACALSLGDFT
AIALFGQADFTSLPHLLYQQLGHYRSQEAAVTAFILLVFCLSVFMIIERHQEPRDD
>Mature_536_residues
MFKRFRAFTYRPASYLGGMLVIVFLIAFYAFALGAVFSLPFVRSWTALLSDQYLQHVIIFSFWQAFLSAVLAVLFGVIVA
RAFFYQPFVGKKLILKLFSLTFVLPALVVIFGLLGVYGASGWLAMLSQFFAWDWTPNIYGLTGILLAHLFFNVPLACRLF
LQGLQAIPVQQRQLAAQLNLRAWHFIRLIEWPYLRQQLLPAFTLIFMLCFTSFAIVLTLGGGPKYTTLEVAIYQAILFEF
DVPKAGLFALLQFVFCFLLFTLSSFFSPAPATTLHSQPTWFAPQSYWVKLWQRMIIVCATVFILLPLLNTLVSALLSPQF
FTLWLQPQLWKALGYSLTIAPTSALLALVLSFAVLLLARELHWRHYRSLSHVILNIGATILAIPTLVLAIGLFILLREID
FSPYHLFGVVVCCNALAAMPFVLRILALPMHNNMIYYEKLCQSLNLRGWQRFRLIEWHKLRAPMKYAFALACALSLGDFT
AIALFGQADFTSLPHLLYQQLGHYRSQEAAVTAFILLVFCLSVFMIIERHQEPRDD

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=494, Percent_Identity=47.3684210526316, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 61042; Mature: 61042

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]