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The map label for this gene is parE [H]

Identifier: 15602235

GI number: 15602235

Start: 433699

End: 435597

Strand: Reverse

Name: parE [H]

Synonym: PM0370

Alternate gene names: 15602235

Gene position: 435597-433699 (Counterclockwise)

Preceding gene: 15602240

Following gene: 15602234

Centisome position: 19.3

GC content: 44.29

Gene sequence:

>1899_bases
ATGACAAGCAATTATTCCGCACAAGAAATTACTGTTCTAAAAGATTTGGAGCCAGTGCAACTTCGCCCCGGCATGTATAC
CGATACCACCCGTCCCAACCATTTAGGTCAAGAAGTCATTGACAACAGTGTTGATGAGGCATTAGCTGGCTATGCAAATA
AAATTGAAGTGGTCCTACACAAAGATCAATCCTTGGAAGTCATTGATAACGGGCGAGGCATGCCTGTGGATATTCATCCC
GTAGAAAAGCTATCGGGTGTTGAAGTGATTTTGACCAAATTACATGCTGGTGGAAAATTCTCAAATAAAAACTATGAATT
CTCCGGTGGTTTGCATGGTGTAGGTATTTCTGTGGTCAATGCGCTTTCTGAACGGGTTGACGTGACAGTAAAACGCAATG
GTGAGGTCTATAAAATCGCCTTTGAAAACGGTCGAAAAGTCGAAGAATTAGAGGTTATTGGTACTTGTGGCAGACGTAAC
ACAGGGACAACGGTACATTTCAAACCCAATCCGAAGTATTTTGACAGCGATAAATTTTCGGTTTCGCGTTTACGCCATTT
ATTGCGTGCTAAAGCAGTCCTCTGCTCCGGCTTAGAAATTAAGTTCATTGACAAAATCAATCAAACTGAAGACTGTTGGT
GCTACGAAGATGGGCTCGCGGACTATTTAAGTGAAGCAGTGAATGGTCTTGAAACCCTACCGGAAACGCCATTTATTGGT
GATTTTAAAGGCGATACAGAAGCGGTCAGTTGGGCATTATTATGGCTTCCGGAAGGCGGTGACCTATTGAGCGAAAGCTA
TGTCAACTTAATCCCAACGATCCAAGGCGGTACCCACGTCAATGGACTACGTCAAGGCTTACTGGACGCCATGCGTGAGT
TCTGTGAATTTCGTAATCTACTACCAAGAGGTGTTAAACTCAGCGCCGACGATATCTGGGAGCGTTGCGCCTATGTCCTT
TCGCTGAAAATGCAAGATCCGCAATTTGCTGGACAAACCAAAGAGCGCCTATCTTCGCGCCAAAGTGCGGTATTTGTTGC
TGGCGTTGTTAAAGATGCCTTCAGCTTATGGTTGAATCAACATGTACAACAAGCCGAACTTTTGGCTGAAATGGCAATTA
GCTCTGCACAACGTCGTTTACGCGCCGCCAAAAAAGTCGTACGTAAAAAATTAGTGAGTGGTCCTGCCTTACCCGGGAAA
TTAGCGGATTGTAGCCAACAAGATTTAGACAAAACCGAATTATTTTTAGTGGAAGGGGATTCTGCCGGTGGTTCTGCTAA
ACAAGCACGTGATCGCGAATACCAAGCAATTTTGCCGTTACGAGGGAAAATTCTCAATACTTGGGAAGTCTCTTCTGAGC
AAGTTCTCTCTTCAAGTGAAATCCATGATATTGCAGTGGCACTTGGGATTGATCCTGACAACGAAGACTTATCTCAACTG
CGCTATGGTAAAGTATGCATTCTTGCCGATGCCGACTCCGATGGGCTACATATTGCGACCCTACTTTGTGCCTTATTTTT
ACGCCACTTCCCGAAATTGGTACAAAAGGGACATGTTTATGTTGCGATGCCACCGCTTTACCGTATTGACTTAGGTAAAG
ACGTCTTTTACGCCCTTGATGAAAGCGAAAAAGACGCCATTTTAGATCGCTTAAAAGGCAAAAAAGGTAAACCGAACGTA
CAACGTTTTAAAGGGTTAGGTGAAATGAACCCACTACAATTACGCGAAACTACGATGGATCCCAATACGCGCCGGTTAGT
GCAATTGACCTATGCTCTTGAACAAGAAGAACAAGCCGCAACCCTTGAACTGATGGATATGCTCCTCGCCAAAAAGCGCG
CAGAAGATCGTAAAAATTGGTTGCAAACAAAAGGGGATCAGGTGGATTTAGCCGTATAA

Upstream 100 bases:

>100_bases
AATGAATGATTGCCCCCTTGAGTATAGTGCAAAAATACACATTTTTTGCTACACTCGTTGGAACTTTTGACACAAAAAAT
AAAATAATGAACAACGCATG

Downstream 100 bases:

>100_bases
AGATGATGGCGACTTGCTTTTCTTATGCAAATAAAGTCGCCACACACCGATTAAGTGGCTTCCTGATATCAAGAACATTT
TTTAACGGAAAATACCTTGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTSNYSAQEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALAGYANKIEVVLHKDQSLEVIDNGRGMPVDIHP
VEKLSGVEVILTKLHAGGKFSNKNYEFSGGLHGVGISVVNALSERVDVTVKRNGEVYKIAFENGRKVEELEVIGTCGRRN
TGTTVHFKPNPKYFDSDKFSVSRLRHLLRAKAVLCSGLEIKFIDKINQTEDCWCYEDGLADYLSEAVNGLETLPETPFIG
DFKGDTEAVSWALLWLPEGGDLLSESYVNLIPTIQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLSADDIWERCAYVL
SLKMQDPQFAGQTKERLSSRQSAVFVAGVVKDAFSLWLNQHVQQAELLAEMAISSAQRRLRAAKKVVRKKLVSGPALPGK
LADCSQQDLDKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSSEQVLSSSEIHDIAVALGIDPDNEDLSQL
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQKGHVYVAMPPLYRIDLGKDVFYALDESEKDAILDRLKGKKGKPNV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTYALEQEEQAATLELMDMLLAKKRAEDRKNWLQTKGDQVDLAV

Sequences:

>Translated_632_residues
MTSNYSAQEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALAGYANKIEVVLHKDQSLEVIDNGRGMPVDIHP
VEKLSGVEVILTKLHAGGKFSNKNYEFSGGLHGVGISVVNALSERVDVTVKRNGEVYKIAFENGRKVEELEVIGTCGRRN
TGTTVHFKPNPKYFDSDKFSVSRLRHLLRAKAVLCSGLEIKFIDKINQTEDCWCYEDGLADYLSEAVNGLETLPETPFIG
DFKGDTEAVSWALLWLPEGGDLLSESYVNLIPTIQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLSADDIWERCAYVL
SLKMQDPQFAGQTKERLSSRQSAVFVAGVVKDAFSLWLNQHVQQAELLAEMAISSAQRRLRAAKKVVRKKLVSGPALPGK
LADCSQQDLDKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSSEQVLSSSEIHDIAVALGIDPDNEDLSQL
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQKGHVYVAMPPLYRIDLGKDVFYALDESEKDAILDRLKGKKGKPNV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTYALEQEEQAATLELMDMLLAKKRAEDRKNWLQTKGDQVDLAV
>Mature_631_residues
TSNYSAQEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALAGYANKIEVVLHKDQSLEVIDNGRGMPVDIHPV
EKLSGVEVILTKLHAGGKFSNKNYEFSGGLHGVGISVVNALSERVDVTVKRNGEVYKIAFENGRKVEELEVIGTCGRRNT
GTTVHFKPNPKYFDSDKFSVSRLRHLLRAKAVLCSGLEIKFIDKINQTEDCWCYEDGLADYLSEAVNGLETLPETPFIGD
FKGDTEAVSWALLWLPEGGDLLSESYVNLIPTIQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLSADDIWERCAYVLS
LKMQDPQFAGQTKERLSSRQSAVFVAGVVKDAFSLWLNQHVQQAELLAEMAISSAQRRLRAAKKVVRKKLVSGPALPGKL
ADCSQQDLDKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSSEQVLSSSEIHDIAVALGIDPDNEDLSQLR
YGKVCILADADSDGLHIATLLCALFLRHFPKLVQKGHVYVAMPPLYRIDLGKDVFYALDESEKDAILDRLKGKKGKPNVQ
RFKGLGEMNPLQLRETTMDPNTRRLVQLTYALEQEEQAATLELMDMLLAKKRAEDRKNWLQTKGDQVDLAV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=579, Percent_Identity=26.2521588946459, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19913406, Length=643, Percent_Identity=25.194401244168, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=78.1645569620253, Blast_Score=1053, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=39.572192513369, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535065, Length=627, Percent_Identity=26.1562998405104, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=608, Percent_Identity=25.1644736842105, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=232, Percent_Identity=28.0172413793103, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=652, Percent_Identity=25.4601226993865, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17136538, Length=640, Percent_Identity=23.4375, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70365; Mature: 70234

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNYSAQEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALAGYANKIEVVLH
CCCCCCCCEEEEECCCCCCEECCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCEEEEEEE
KDQSLEVIDNGRGMPVDIHPVEKLSGVEVILTKLHAGGKFSNKNYEFSGGLHGVGISVVN
CCCCEEEEECCCCCCEECCHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSERVDVTVKRNGEVYKIAFENGRKVEELEVIGTCGRRNTGTTVHFKPNPKYFDSDKFS
HHHHHEEEEEECCCCEEEEEECCCCCEEHHHHEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLRHLLRAKAVLCSGLEIKFIDKINQTEDCWCYEDGLADYLSEAVNGLETLPETPFIG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
DFKGDTEAVSWALLWLPEGGDLLSESYVNLIPTIQGGTHVNGLRQGLLDAMREFCEFRNL
CCCCCCHHEEEEEEEECCCCCHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLSADDIWERCAYVLSLKMQDPQFAGQTKERLSSRQSAVFVAGVVKDAFSLWLNQ
CCCCCEECHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
HVQQAELLAEMAISSAQRRLRAAKKVVRKKLVSGPALPGKLADCSQQDLDKTELFLVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREYQAILPLRGKILNTWEVSSEQVLSSSEIHDIAVALGIDPDNEDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEECCCHHHHHCCCCCEEEEEEEECCCCCHHHHHH
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQKGHVYVAMPPLYRIDLGKDVFYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
ESEKDAILDRLKGKKGKPNVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTYALEQEEQAA
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
TLELMDMLLAKKRAEDRKNWLQTKGDQVDLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TSNYSAQEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALAGYANKIEVVLH
CCCCCCCEEEEECCCCCCEECCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCEEEEEEE
KDQSLEVIDNGRGMPVDIHPVEKLSGVEVILTKLHAGGKFSNKNYEFSGGLHGVGISVVN
CCCCEEEEECCCCCCEECCHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSERVDVTVKRNGEVYKIAFENGRKVEELEVIGTCGRRNTGTTVHFKPNPKYFDSDKFS
HHHHHEEEEEECCCCEEEEEECCCCCEEHHHHEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLRHLLRAKAVLCSGLEIKFIDKINQTEDCWCYEDGLADYLSEAVNGLETLPETPFIG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
DFKGDTEAVSWALLWLPEGGDLLSESYVNLIPTIQGGTHVNGLRQGLLDAMREFCEFRNL
CCCCCCHHEEEEEEEECCCCCHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLSADDIWERCAYVLSLKMQDPQFAGQTKERLSSRQSAVFVAGVVKDAFSLWLNQ
CCCCCEECHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
HVQQAELLAEMAISSAQRRLRAAKKVVRKKLVSGPALPGKLADCSQQDLDKTELFLVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREYQAILPLRGKILNTWEVSSEQVLSSSEIHDIAVALGIDPDNEDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEECCCHHHHHCCCCCEEEEEEEECCCCCHHHHHH
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQKGHVYVAMPPLYRIDLGKDVFYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
ESEKDAILDRLKGKKGKPNVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTYALEQEEQAA
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
TLELMDMLLAKKRAEDRKNWLQTKGDQVDLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]