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The map label for this gene is serS

Identifier: 15602123

GI number: 15602123

Start: 290295

End: 291581

Strand: Direct

Name: serS

Synonym: PM0258

Alternate gene names: 15602123

Gene position: 290295-291581 (Clockwise)

Preceding gene: 15602122

Following gene: 15602130

Centisome position: 12.86

GC content: 40.33

Gene sequence:

>1287_bases
ATGATCGATCCAAATCTATTGCGTAATAATCTTGAGGAAGTGGCTGAAAAATTAAAAGTAAAACGTAATTTTGTGCTAGA
TGTTGCCTTATTAAGTGAATTAGAAGAACAACGTAAAAGCTTGCAAGTCAAAACAGAATCACTTCAAGCAGAACGTAATG
CGCGTTCAAAAAATATCGGCCAAGCGAAAGCACGTGGTGAAGATATTTCAGCATTATTAAACGAAGTGGAACATATGGGC
ATTGAATTAAGTACCACAAAAGCACATTTGGATGAAGTGTTAGCTGAAATTAACCAGATCGTTCTCGCCATTCCAAATTT
ACCTGCTGATGAAGTTCCGCTTGGCAAAGATGAGAGCGAAAATCTCGAAGTGTTCCGTTGGGGAACACCAAAAACCTTTG
ATTTTGATGTGAAAGATCATGTGTCATTAGGTGAAGGTTTGAAAGGGCTAGATTTTGCGGCAGGAGTAAAATTAAGTGGC
TCTCGTTTTGTGGTGATGAAAGGACAGATCGCCAAATTACATCGTGCGCTGTCTCAATTTATGTTAGACCTCCATACTGA
GCAACATGGTTATACCGAAACCTATGTCCCTTATTTAGTTAATCACGCTACTTTATATGGTACCGGGCAATTACCAAAAT
TTGGTGAGGATTTATTTCACACCAATGCGTTAGAAGGCGAACAACCTTATGCATTAATTCCAACAGCAGAAGTACCCGTT
ACGAACTTAGTTCGTGATGAAATTCTCGATGAAGCGGATTTGCCTTTGAAAATGACAGCGCATACTCCTTGTTTCCGTTC
TGAAGCAGGATCCTATGGGCGTGATACCCGCGGTTTAATTCGTATGCATCAATTTGATAAAGTGGAGTTGGTTCAAATTG
TTGCACCTGAAACTTCAATGCAAGTGTTAGAAGAATTGACAGGACAAGCAGAAAAAGTCTTGCAATTATTAGAGTTACCT
TATCGTAAAGTCTTATTATGTACAGGCGATATGGGATTCGGCTCATGCAAGACGTATGACTTAGAAGTATGGTTACCGGC
TCAAAATACCTATCGTGAGATCTCTTCATGTTCAAATATGTGGGATTTCCAAGCTCGTCGCATGCAAGCGCGTTGCCGTT
CTAAAACAGATAAGAAAACACGTTTAGTGCATACTTTAAACGGCTCTGGCTTAGCGGTTGGACGCACTTTGGTCGCGATT
TTAGAAAATTACCAAAATGCAGATGGTACCATTACCGTTCCAAGCGTATTACGTCCATATATGGGCGGGTTAGCACAGAT
TGGTTAG

Upstream 100 bases:

>100_bases
TAAACTGGTTTATTAGGTTAATTCGATCTAAAATAGATGAATTATTTTTATGCAAAAGTGCGGTTAATTCCGTTTAAATT
TTAAGAAAAGTAGGTAAAAA

Downstream 100 bases:

>100_bases
TCATTTCGCGTTTAAAAAAGCGGTCCCTTGTTAAAGAGGTGACCGCTTTTTTCATTCTATTTTTGTGTGGTTTACAGCGC
AATATAATCAAGTGGGATAT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIDPNLLRNNLEEVAEKLKVKRNFVLDVALLSELEEQRKSLQVKTESLQAERNARSKNIGQAKARGEDISALLNEVEHMG
IELSTTKAHLDEVLAEINQIVLAIPNLPADEVPLGKDESENLEVFRWGTPKTFDFDVKDHVSLGEGLKGLDFAAGVKLSG
SRFVVMKGQIAKLHRALSQFMLDLHTEQHGYTETYVPYLVNHATLYGTGQLPKFGEDLFHTNALEGEQPYALIPTAEVPV
TNLVRDEILDEADLPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVELVQIVAPETSMQVLEELTGQAEKVLQLLELP
YRKVLLCTGDMGFGSCKTYDLEVWLPAQNTYREISSCSNMWDFQARRMQARCRSKTDKKTRLVHTLNGSGLAVGRTLVAI
LENYQNADGTITVPSVLRPYMGGLAQIG

Sequences:

>Translated_428_residues
MIDPNLLRNNLEEVAEKLKVKRNFVLDVALLSELEEQRKSLQVKTESLQAERNARSKNIGQAKARGEDISALLNEVEHMG
IELSTTKAHLDEVLAEINQIVLAIPNLPADEVPLGKDESENLEVFRWGTPKTFDFDVKDHVSLGEGLKGLDFAAGVKLSG
SRFVVMKGQIAKLHRALSQFMLDLHTEQHGYTETYVPYLVNHATLYGTGQLPKFGEDLFHTNALEGEQPYALIPTAEVPV
TNLVRDEILDEADLPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVELVQIVAPETSMQVLEELTGQAEKVLQLLELP
YRKVLLCTGDMGFGSCKTYDLEVWLPAQNTYREISSCSNMWDFQARRMQARCRSKTDKKTRLVHTLNGSGLAVGRTLVAI
LENYQNADGTITVPSVLRPYMGGLAQIG
>Mature_428_residues
MIDPNLLRNNLEEVAEKLKVKRNFVLDVALLSELEEQRKSLQVKTESLQAERNARSKNIGQAKARGEDISALLNEVEHMG
IELSTTKAHLDEVLAEINQIVLAIPNLPADEVPLGKDESENLEVFRWGTPKTFDFDVKDHVSLGEGLKGLDFAAGVKLSG
SRFVVMKGQIAKLHRALSQFMLDLHTEQHGYTETYVPYLVNHATLYGTGQLPKFGEDLFHTNALEGEQPYALIPTAEVPV
TNLVRDEILDEADLPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVELVQIVAPETSMQVLEELTGQAEKVLQLLELP
YRKVLLCTGDMGFGSCKTYDLEVWLPAQNTYREISSCSNMWDFQARRMQARCRSKTDKKTRLVHTLNGSGLAVGRTLVAI
LENYQNADGTITVPSVLRPYMGGLAQIG

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=333, Percent_Identity=36.9369369369369, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI8923421, Length=427, Percent_Identity=28.3372365339578, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI225579074, Length=324, Percent_Identity=30.8641975308642, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787120, Length=431, Percent_Identity=77.4941995359629, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17539260, Length=432, Percent_Identity=31.0185185185185, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17542084, Length=330, Percent_Identity=28.7878787878788, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320226, Length=397, Percent_Identity=31.7380352644836, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321799, Length=406, Percent_Identity=29.064039408867, Blast_Score=173, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24581334, Length=465, Percent_Identity=30.3225806451613, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17737985, Length=263, Percent_Identity=34.6007604562738, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_PASMU (P57836)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245195.1
- ProteinModelPortal:   P57836
- SMR:   P57836
- GeneID:   1243605
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0258
- NMPDR:   fig|272843.1.peg.258
- HOGENOM:   HBG629391
- OMA:   PKFADDM
- ProtClustDB:   PRK05431
- BioCyc:   PMUL272843:PM0258-MONOMER
- BRENDA:   6.1.1.11
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 47791; Mature: 47791

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 289-289 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPNLLRNNLEEVAEKLKVKRNFVLDVALLSELEEQRKSLQVKTESLQAERNARSKNIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QAKARGEDISALLNEVEHMGIELSTTKAHLDEVLAEINQIVLAIPNLPADEVPLGKDESE
HHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
NLEVFRWGTPKTFDFDVKDHVSLGEGLKGLDFAAGVKLSGSRFVVMKGQIAKLHRALSQF
CEEEEECCCCCEEECCCHHHHHHCCCCCCCCHHCCEEECCCEEEEECCHHHHHHHHHHHH
MLDLHTEQHGYTETYVPYLVNHATLYGTGQLPKFGEDLFHTNALEGEQPYALIPTAEVPV
HHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCH
TNLVRDEILDEADLPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVELVQIVAPETSM
HHHHHHHHHCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCHHH
QVLEELTGQAEKVLQLLELPYRKVLLCTGDMGFGSCKTYDLEVWLPAQNTYREISSCSNM
HHHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHH
WDFQARRMQARCRSKTDKKTRLVHTLNGSGLAVGRTLVAILENYQNADGTITVPSVLRPY
HHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHH
MGGLAQIG
HHHHHCCC
>Mature Secondary Structure
MIDPNLLRNNLEEVAEKLKVKRNFVLDVALLSELEEQRKSLQVKTESLQAERNARSKNIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QAKARGEDISALLNEVEHMGIELSTTKAHLDEVLAEINQIVLAIPNLPADEVPLGKDESE
HHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
NLEVFRWGTPKTFDFDVKDHVSLGEGLKGLDFAAGVKLSGSRFVVMKGQIAKLHRALSQF
CEEEEECCCCCEEECCCHHHHHHCCCCCCCCHHCCEEECCCEEEEECCHHHHHHHHHHHH
MLDLHTEQHGYTETYVPYLVNHATLYGTGQLPKFGEDLFHTNALEGEQPYALIPTAEVPV
HHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCH
TNLVRDEILDEADLPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVELVQIVAPETSM
HHHHHHHHHCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCHHH
QVLEELTGQAEKVLQLLELPYRKVLLCTGDMGFGSCKTYDLEVWLPAQNTYREISSCSNM
HHHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHH
WDFQARRMQARCRSKTDKKTRLVHTLNGSGLAVGRTLVAILENYQNADGTITVPSVLRPY
HHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHH
MGGLAQIG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100