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The map label for this gene is metF [H]

Identifier: 15602100

GI number: 15602100

Start: 264664

End: 265551

Strand: Direct

Name: metF [H]

Synonym: PM0235

Alternate gene names: 15602100

Gene position: 264664-265551 (Clockwise)

Preceding gene: 15602090

Following gene: 15602101

Centisome position: 11.72

GC content: 41.44

Gene sequence:

>888_bases
ATGAGTTACGCAAAAGAGATTGATACACTCAATCAACATATTGCAGATTTGAACGGTAAGATAAATGTGTCCTTTGAATT
TTTCCCGCCGAAAAATGAAAAAATGGAAAGTATTTTATGGGATTCGATTCATCGTTTAAAAGTGCTGAAACCCAAGTTTG
TCTCCGTGACATATGGCGCAAATTCTGGTGAGCGCGATCGCACTCATTCCGTAGTAAAAAATATTAAACAGCAAACGGGG
TTAGAAGCCGCACCACATTTAACGGGGATTGATGCCACACCGGAAGAACTCATTGAAATTGCGAAAGATTATTGGAACAG
TGGGATTCGTCGTATTGTGGCATTACGTGGTGATGAGCCAAAAGGCTATGCGAAAAAGCCCTTTTACGCTTCAGATCTTG
TTGCGCTGTTACGCTCAGTGGCGGATTTTGATATCTCTGTGGCGGCTTATCCAGAAGTCCATCCAGAAGCCAAATCAGCA
CAAGCGGATTTGATCAATTTAAAGCGTAAAATTGATGCAGGTGCTAACCATGTAATTACCCAATTCTTTTTTGATATTGA
CAGTTATCTGCGTTTCCGTGATCGCTGTGCTTCGATTGGGATTGACGCAGAAATTGTGCCGGGTATTTTACCCGTGACGA
ATTTTAAGCAATTGGTCAAAATGGCAGCAATTACCAATGTTAAAATTCCCGCTTGGTTAGCGAAGAATTATGAGGGATTA
GATGATGATCCTACCACACGTAATTTAGTGGCGGCGAGTGTGGCGATGGATATGGTCAGAGTTCTTTCTCGCGAAGGTGT
TAATGACTTCCATTTTTATACTTTGAATCGGAGTGAGTTGACTTATGCCATTTGCCATACTTTAGGTATTCGTCCGAGTA
TTGAATAG

Upstream 100 bases:

>100_bases
ATTCATTTTTGTAATGAAATTTTTGAAATTGACACATATACCGTTTCTCATTACTTTAGCCATATAGACATCTAAATCAG
TTTTGTATATGAGGTAAGTC

Downstream 100 bases:

>100_bases
CCAACTGACTCGTCAATGGTAAGAAAAAGACAAATGCAACACAAAAAACGAATGATTTTGTATAGTATGCTGTGAAATTT
GCGAAAAAGAGAGAATCATC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSYAKEIDTLNQHIADLNGKINVSFEFFPPKNEKMESILWDSIHRLKVLKPKFVSVTYGANSGERDRTHSVVKNIKQQTG
LEAAPHLTGIDATPEELIEIAKDYWNSGIRRIVALRGDEPKGYAKKPFYASDLVALLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIDSYLRFRDRCASIGIDAEIVPGILPVTNFKQLVKMAAITNVKIPAWLAKNYEGL
DDDPTTRNLVAASVAMDMVRVLSREGVNDFHFYTLNRSELTYAICHTLGIRPSIE

Sequences:

>Translated_295_residues
MSYAKEIDTLNQHIADLNGKINVSFEFFPPKNEKMESILWDSIHRLKVLKPKFVSVTYGANSGERDRTHSVVKNIKQQTG
LEAAPHLTGIDATPEELIEIAKDYWNSGIRRIVALRGDEPKGYAKKPFYASDLVALLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIDSYLRFRDRCASIGIDAEIVPGILPVTNFKQLVKMAAITNVKIPAWLAKNYEGL
DDDPTTRNLVAASVAMDMVRVLSREGVNDFHFYTLNRSELTYAICHTLGIRPSIE
>Mature_294_residues
SYAKEIDTLNQHIADLNGKINVSFEFFPPKNEKMESILWDSIHRLKVLKPKFVSVTYGANSGERDRTHSVVKNIKQQTGL
EAAPHLTGIDATPEELIEIAKDYWNSGIRRIVALRGDEPKGYAKKPFYASDLVALLRSVADFDISVAAYPEVHPEAKSAQ
ADLINLKRKIDAGANHVITQFFFDIDSYLRFRDRCASIGIDAEIVPGILPVTNFKQLVKMAAITNVKIPAWLAKNYEGLD
DDPTTRNLVAASVAMDMVRVLSREGVNDFHFYTLNRSELTYAICHTLGIRPSIE

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=289, Percent_Identity=33.9100346020761, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1790377, Length=292, Percent_Identity=72.6027397260274, Blast_Score=462, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=30.2158273381295, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=30.2158273381295, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6325234, Length=279, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=29.4701986754967, Blast_Score=132, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33065; Mature: 32934

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAKEIDTLNQHIADLNGKINVSFEFFPPKNEKMESILWDSIHRLKVLKPKFVSVTYGA
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHSVVKNIKQQTGLEAAPHLTGIDATPEELIEIAKDYWNSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHEECCCCC
KGYAKKPFYASDLVALLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIDSYLRFRDRCASIGIDAEIVPGILPVTNFKQLVKMAAITNVKIPAWLAKNYEGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
DDDPTTRNLVAASVAMDMVRVLSREGVNDFHFYTLNRSELTYAICHTLGIRPSIE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SYAKEIDTLNQHIADLNGKINVSFEFFPPKNEKMESILWDSIHRLKVLKPKFVSVTYGA
CHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHSVVKNIKQQTGLEAAPHLTGIDATPEELIEIAKDYWNSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHEECCCCC
KGYAKKPFYASDLVALLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIDSYLRFRDRCASIGIDAEIVPGILPVTNFKQLVKMAAITNVKIPAWLAKNYEGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
DDDPTTRNLVAASVAMDMVRVLSREGVNDFHFYTLNRSELTYAICHTLGIRPSIE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]