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The map label for this gene is glyA

Identifier: 15602090

GI number: 15602090

Start: 251089

End: 252351

Strand: Direct

Name: glyA

Synonym: PM0225

Alternate gene names: 15602090

Gene position: 251089-252351 (Clockwise)

Preceding gene: 15602089

Following gene: 15602100

Centisome position: 11.12

GC content: 44.18

Gene sequence:

>1263_bases
ATGTTAAAAAGAAGTATGAACATCGCTGATTACGATCCGGTTTTATGGCAGGCAATTCAAGACGAAAATCGCCGTCAAGA
AGAACACATTGAATTAATTGCATCAGAAAACTATGCCAGCCCACGCGTGATGGAAGCACAGGGTTCTCAATTTACCAATA
AATATGCGGAAGGTTACCCGGGTAAACGTTATTATGGTGGTTGTGAATATGCAGATATCGTTGAGCAATTAGCGATTGAT
CGTGCAAAAGAGTTATTTCATGCGGATTATGTGAACGTACAACCCCACTCGGGTTCGCAAGCTAACGCGGCAGTGTATGG
TGCCTTATTACAGCCACATGACACTATTCTTGGTATGAGCCTTGCACACGGGGGACACTTAACGCACGGTGCATCAGTGA
GCTTTTCGGGTAAAATCTATAATGCAGTGCAATATGGAATTACTGCCGAAGGTTTAATTGATTATGAAGATGTGCGTCAA
AAAGCCTTAGAGTGCAAGCCAAAAATGATCGTGGCGGGTTTCTCTGCCTATTCGCAAGTAGTGGATTGGGCGAAAATGCG
TGAAATCGCTGATGAAGTGGGAGCGTATCTTTTCGTGGATATGGCGCACGTGGCAGGGCTGATTGCAGCAGGTGTTTACC
CAAGTCCGTTACCACATGCACATGTTGTGACTACTACCACACACAAAACCTTAGGGGGACCTCGTGGTGGTTTAATTCTA
TCGGCTGCGAAAGATGAAGATCTTTATAAAAAATTACAAAGTTCTGTTTTCCCTGCCAACCAAGGTGGTCCGTTAGTGCA
TGTGATCGCCGCAAAAGCGGTCTGTTTTAAAGAAGCGCTTGAGCCTGAATACAAAGTGTATCAACAACAAGTGGTGAAAA
ATGCGAAAGCCATGGTGGACGTGTTTAAACAACGTGGTTATAACGTGGTATCCAATGGCACAGAAAACCATTTATTCTTA
GTAGATTTAGTTAGCCACGGTTTAACCGGTAAAGCAGCAGATGCTGCGTTAGGCAGTGCAAACATTACCGTCAACAAAAA
TGCGGTACCAAACGATCCACAAAAACCATTTGTGACCTCAGGTATCCGTGTGGGCACACCATCTATCACCCGTCGTGGCT
TTAAAGAAGCTGAATCGGCAGAATTAGCCGGTTGGATGTGTGATGTGTTGGATGCGATGGGTAAAGACAACGAAGCACAA
GTGATTGCACAGACAAAAGAGAAAGTCTTAGCAATTTGTAAACGTCTTCCTGTTTATGCTTAA

Upstream 100 bases:

>100_bases
AAAATGAAAAAATTCCCACCACATTTTCATTAAAAAATAGCGTGTAAACGAGTACACTAAACAAATTCACCATCATCTTT
ACTTAAAAGAGGACATCATA

Downstream 100 bases:

>100_bases
TTGACTTTTACTTGAGAACGACATAAGCGGTGTTTATCACCGCTTATTTTTTTGCCTTAATCTTTTGATAGCCGTAAATA
GACAAAATAAAAAAACGGTC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLKRSMNIADYDPVLWQAIQDENRRQEEHIELIASENYASPRVMEAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKELFHADYVNVQPHSGSQANAAVYGALLQPHDTILGMSLAHGGHLTHGASVSFSGKIYNAVQYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQVVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTTTTHKTLGGPRGGLIL
SAAKDEDLYKKLQSSVFPANQGGPLVHVIAAKAVCFKEALEPEYKVYQQQVVKNAKAMVDVFKQRGYNVVSNGTENHLFL
VDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTSGIRVGTPSITRRGFKEAESAELAGWMCDVLDAMGKDNEAQ
VIAQTKEKVLAICKRLPVYA

Sequences:

>Translated_420_residues
MLKRSMNIADYDPVLWQAIQDENRRQEEHIELIASENYASPRVMEAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKELFHADYVNVQPHSGSQANAAVYGALLQPHDTILGMSLAHGGHLTHGASVSFSGKIYNAVQYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQVVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTTTTHKTLGGPRGGLIL
SAAKDEDLYKKLQSSVFPANQGGPLVHVIAAKAVCFKEALEPEYKVYQQQVVKNAKAMVDVFKQRGYNVVSNGTENHLFL
VDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTSGIRVGTPSITRRGFKEAESAELAGWMCDVLDAMGKDNEAQ
VIAQTKEKVLAICKRLPVYA
>Mature_420_residues
MLKRSMNIADYDPVLWQAIQDENRRQEEHIELIASENYASPRVMEAQGSQFTNKYAEGYPGKRYYGGCEYADIVEQLAID
RAKELFHADYVNVQPHSGSQANAAVYGALLQPHDTILGMSLAHGGHLTHGASVSFSGKIYNAVQYGITAEGLIDYEDVRQ
KALECKPKMIVAGFSAYSQVVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTTTTHKTLGGPRGGLIL
SAAKDEDLYKKLQSSVFPANQGGPLVHVIAAKAVCFKEALEPEYKVYQQQVVKNAKAMVDVFKQRGYNVVSNGTENHLFL
VDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTSGIRVGTPSITRRGFKEAESAELAGWMCDVLDAMGKDNEAQ
VIAQTKEKVLAICKRLPVYA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=392, Percent_Identity=47.7040816326531, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862350, Length=392, Percent_Identity=47.7040816326531, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862348, Length=392, Percent_Identity=47.7040816326531, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI19923315, Length=392, Percent_Identity=47.7040816326531, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862346, Length=391, Percent_Identity=46.5473145780051, Blast_Score=341, Evalue=9e-94,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=45.1371571072319, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=43.4447300771208, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1788902, Length=420, Percent_Identity=77.3809523809524, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=44.4444444444444, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=44.4444444444444, Blast_Score=324, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=44.5273631840796, Blast_Score=336, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=445, Percent_Identity=41.123595505618, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=47, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=47, Blast_Score=358, Evalue=5e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PASMU (P57830)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245162.1
- ProteinModelPortal:   P57830
- SMR:   P57830
- GeneID:   1243572
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0225
- NMPDR:   fig|272843.1.peg.225
- HOGENOM:   HBG301263
- OMA:   ADVEANV
- ProtClustDB:   PRK00011
- BioCyc:   PMUL272843:PM0225-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45684; Mature: 45684

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRSMNIADYDPVLWQAIQDENRRQEEHIELIASENYASPRVMEAQGSQFTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCC
GKRYYGGCEYADIVEQLAIDRAKELFHADYVNVQPHSGSQANAAVYGALLQPHDTILGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCHHHHEEH
LAHGGHLTHGASVSFSGKIYNAVQYGITAEGLIDYEDVRQKALECKPKMIVAGFSAYSQV
HCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTTTTHKTLGGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHCCCCCCCEEE
SAAKDEDLYKKLQSSVFPANQGGPLVHVIAAKAVCFKEALEPEYKVYQQQVVKNAKAMVD
EECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFKQRGYNVVSNGTENHLFLVDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTS
HHHHCCCEEECCCCCCCEEEEEHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
GIRVGTPSITRRGFKEAESAELAGWMCDVLDAMGKDNEAQVIAQTKEKVLAICKRLPVYA
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKRSMNIADYDPVLWQAIQDENRRQEEHIELIASENYASPRVMEAQGSQFTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCC
GKRYYGGCEYADIVEQLAIDRAKELFHADYVNVQPHSGSQANAAVYGALLQPHDTILGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCHHHHEEH
LAHGGHLTHGASVSFSGKIYNAVQYGITAEGLIDYEDVRQKALECKPKMIVAGFSAYSQV
HCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTTTTHKTLGGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHCCCCCCCEEE
SAAKDEDLYKKLQSSVFPANQGGPLVHVIAAKAVCFKEALEPEYKVYQQQVVKNAKAMVD
EECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFKQRGYNVVSNGTENHLFLVDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTS
HHHHCCCEEECCCCCCCEEEEEHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
GIRVGTPSITRRGFKEAESAELAGWMCDVLDAMGKDNEAQVIAQTKEKVLAICKRLPVYA
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100