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The map label for this gene is purD

Identifier: 15602089

GI number: 15602089

Start: 249672

End: 250961

Strand: Direct

Name: purD

Synonym: PM0224

Alternate gene names: 15602089

Gene position: 249672-250961 (Clockwise)

Preceding gene: 15602088

Following gene: 15602090

Centisome position: 11.06

GC content: 46.36

Gene sequence:

>1290_bases
ATGAATATTTTGATTATTGGAAATGGCGGTCGTGAACATGCTTTGGCTTGGAAAGTCGCGCAGTCACCAATGGCAGAGAA
AGTGTTTGTTGCACCGGGCAATGCAGGAACTGCATTAGAAGAGAAAGTGGAAAATGTTGCGATTTCCGCTACCGATGTGG
AAAAATTAGTGGCATTTGCACAAGCAAACCATATTGGCTTAACCATTGTTGGACCGGAAGCCCCATTGGTCATTGGTGTA
GTTGATGCATTCCGTGCGGCAGGGTTGAAAATTTTCGGACCAACCAAAGCGGCCGCACAATTAGAAGGTTCAAAAGCTTT
TACGAAAGATTTCTTAGCTCGTCATCACATTCCAACGGCGGAATACCAGAACTTTACAGAAGTGGAACCTGCGCTTGCCT
ATTTACGTGAAAAAGGTGCACCAATCGTGGTGAAAGCCGATGGACTTGCTGCTGGGAAAGGGGTGATTGTGGCAATGACC
TTAGACGAAGCAGAAGCCGCAGTGAAAGAGATGCTTTCAGGCAATGCCTTTGGTGAGGCGGGAAGTCGAGTAGTCATCGA
AGAATTTTTAGACGGCGAAGAAGCCAGTTTCATCGTGATGGTGGACGGTAAAAATGTGGAGCCGATGGCAACCAGTCAAG
ACCATAAACGTGTTGGCGAGAATGACACAGGTTTAAATACAGGTGGCATGGGCGCCTATTCGCCTGCACCAGTCGTGACG
CCAGAGATTCATGAGCGTATTATGCAGCAAGTGATTTATCCAACGGTAAATGGTATGGCAGCCGAAGGGAATATTTATAC
CGGATTCCTTTATGCTGGCTTGATGATTATGCCAAATGGTCAACCAAAGGTTATCGAATTTAACTGCCGTTTTGGTGATC
CTGAAACTCAACCAATTATGCTACGTTTAGAATCTGATTTAGTGGAACTTTGTCTTGCGGCATGTGACGGTAAATTAGAC
CAGAAAAAATCGCAATGGTGTGAGCAAGCGTCACTGGGTATCGTGCTTGCAGCGGAAGGTTACCCAGGCGATTATCGCAA
AGGTGATGAAATCACGGGCATTGAAAGTGCGGTCGAAAATCAAAAAGTTTTTTTAGCCGGCGTGGAAAATAAAGACGGCA
AATTAGTCACCAATGGGGGACGTGTCGTGTGCGTGACGGCATTAGGCGACACCGTTTACGATGCGCAACAACAAGCACTG
GCACTGGCTGAACAAGTGAAATGGACAGGACGTTTTTATCGCCGTGATATTGGTTATCGTGCGGTGGCAAGAGAGAAAAA
TAACGCTTAA

Upstream 100 bases:

>100_bases
ATTCATTGATTTTAACGGGTGATTCAGGTTATTTAGAAGTACAAGCCAATCAGCCACCTGCATTTTTTATACCTAAAAAT
CGTTTCAAAAAGGAATAAAT

Downstream 100 bases:

>100_bases
TTGGCATGATGAATTTTTTTCATTATAAAAATGAAAAAATTCCCACCACATTTTCATTAAAAAATAGCGTGTAAACGAGT
ACACTAAACAAATTCACCAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNILIIGNGGREHALAWKVAQSPMAEKVFVAPGNAGTALEEKVENVAISATDVEKLVAFAQANHIGLTIVGPEAPLVIGV
VDAFRAAGLKIFGPTKAAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LDEAEAAVKEMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGENDTGLNTGGMGAYSPAPVVT
PEIHERIMQQVIYPTVNGMAAEGNIYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLESDLVELCLAACDGKLD
QKKSQWCEQASLGIVLAAEGYPGDYRKGDEITGIESAVENQKVFLAGVENKDGKLVTNGGRVVCVTALGDTVYDAQQQAL
ALAEQVKWTGRFYRRDIGYRAVAREKNNA

Sequences:

>Translated_429_residues
MNILIIGNGGREHALAWKVAQSPMAEKVFVAPGNAGTALEEKVENVAISATDVEKLVAFAQANHIGLTIVGPEAPLVIGV
VDAFRAAGLKIFGPTKAAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LDEAEAAVKEMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGENDTGLNTGGMGAYSPAPVVT
PEIHERIMQQVIYPTVNGMAAEGNIYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLESDLVELCLAACDGKLD
QKKSQWCEQASLGIVLAAEGYPGDYRKGDEITGIESAVENQKVFLAGVENKDGKLVTNGGRVVCVTALGDTVYDAQQQAL
ALAEQVKWTGRFYRRDIGYRAVAREKNNA
>Mature_429_residues
MNILIIGNGGREHALAWKVAQSPMAEKVFVAPGNAGTALEEKVENVAISATDVEKLVAFAQANHIGLTIVGPEAPLVIGV
VDAFRAAGLKIFGPTKAAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LDEAEAAVKEMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGENDTGLNTGGMGAYSPAPVVT
PEIHERIMQQVIYPTVNGMAAEGNIYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLESDLVELCLAACDGKLD
QKKSQWCEQASLGIVLAAEGYPGDYRKGDEITGIESAVENQKVFLAGVENKDGKLVTNGGRVVCVTALGDTVYDAQQQAL
ALAEQVKWTGRFYRRDIGYRAVAREKNNA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=51.7647058823529, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=51.7647058823529, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=51.7647058823529, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=51.7647058823529, Blast_Score=418, Evalue=1e-117,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=74.5920745920746, Blast_Score=651, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=42.1176470588235, Blast_Score=320, Evalue=9e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=41.123595505618, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=44.988344988345, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=44.988344988345, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_PASMU (P57829)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245161.1
- ProteinModelPortal:   P57829
- PRIDE:   P57829
- GeneID:   1243571
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0224
- NMPDR:   fig|272843.1.peg.224
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   PMUL272843:PM0224-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45873; Mature: 45873

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHALAWKVAQSPMAEKVFVAPGNAGTALEEKVENVAISATDVEKLVAFA
CEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHEEEEHHHHHHHHHHH
QANHIGLTIVGPEAPLVIGVVDAFRAAGLKIFGPTKAAAQLEGSKAFTKDFLARHHIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCEEEECCCCHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMTLDEAEAAVKEMLSGNAFGEA
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGENDTGLNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHERIMQQVIYPTVNGMAAEGNIYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEEECCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCEEE
LRLESDLVELCLAACDGKLDQKKSQWCEQASLGIVLAAEGYPGDYRKGDEITGIESAVEN
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCC
QKVFLAGVENKDGKLVTNGGRVVCVTALGDTVYDAQQQALALAEQVKWTGRFYRRDIGYR
CEEEEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVAREKNNA
EECCCCCCC
>Mature Secondary Structure
MNILIIGNGGREHALAWKVAQSPMAEKVFVAPGNAGTALEEKVENVAISATDVEKLVAFA
CEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHEEEEHHHHHHHHHHH
QANHIGLTIVGPEAPLVIGVVDAFRAAGLKIFGPTKAAAQLEGSKAFTKDFLARHHIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCEEEECCCCHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMTLDEAEAAVKEMLSGNAFGEA
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRVGENDTGLNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHERIMQQVIYPTVNGMAAEGNIYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEEECCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCEEE
LRLESDLVELCLAACDGKLDQKKSQWCEQASLGIVLAAEGYPGDYRKGDEITGIESAVEN
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCC
QKVFLAGVENKDGKLVTNGGRVVCVTALGDTVYDAQQQALALAEQVKWTGRFYRRDIGYR
CEEEEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVAREKNNA
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100