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The map label for this gene is menF

Identifier: 15601918

GI number: 15601918

Start: 58288

End: 59583

Strand: Direct

Name: menF

Synonym: PM0053

Alternate gene names: 15601918

Gene position: 58288-59583 (Clockwise)

Preceding gene: 15601913

Following gene: 15601919

Centisome position: 2.58

GC content: 42.59

Gene sequence:

>1296_bases
ATGGATAATTTGCAGTCAATCAAAGCACAATTTATTGCACAAATCAATGCTTATCAACCGGAGAAAGATCGCGATATTGT
GGTATTTCAGTGCAATACTGAAACAACCTTTTCTTTGTTAGCTTGGCTGAAAGCACAGCAATATTATCCTCAGTTTTATT
TGCATGGGCGAGATGGTGCGACAAAATGGGCGTCGATTGGTCAGGTTCGTCAGTTCTCTGATGTCAGTGCAGCGGCACAC
TTTATCCAAGAACAGCAGTTGGCTTTGCTCGGTGGATTACAGTTTTATGGTGACGCGTTGTTTGTGCTACCGCGTCTTTT
ATTACAGCAACGTCAAGATGGCATGACGATAACGTTATTCATTGATGGAAAGCAATTCGAGCAAGATAAGCTTGTGGCGC
TCGCTTGTTTAAGCACATTTGAAAAACAAACCGCACTTCAAACGGTGAAGCAAGAGATAAGCCTCATTTCGCAAAAAGCC
GATCAGGCAGAATGGTGCCGTTGGGTTGAACAAGGCTTGCAAAAAATTAAACAAGGCGAGTTAAGTAAAATTGTTTTAGC
GAATGAGCGTTGCTTTAAAACAGCGGCGCCGTTAGCCGCAACGGATTTGTTAGCGGAAAGTGAGAAATATAATCTAGGTT
GTTATCACTTTATGTTAGCAGAAAGTGAACAGCGTGCCTTTATTGGTTCTAGCCCAGAATTATTGTATCGTCGTCACGGT
TTACAATTAAAAACGGAAGCCTTAGCGGGCACCGCGTTTATGGGGGAAGATGAACAACAAAATCAACAGCAAAGTGATTG
GTTGTTGCACGATAAGAAAAACGAATATGAAAATCAATTGGTTGTGGATGGCATTTGCCAAAATTTACAGCCCTTTGTGC
AACAAATTACGATAGAAAAGGTCGAACTTAAAAAACTCCGTAAAGTACAACATTTACGACGCCGAATTTCGGCACAACTA
AAGGCGGGTTGTGGGGATAAAGACATCTTACTTGCGATGCACCCGACTGCAGCGGTGGCGGGTTTACCTCAATTGGAAGC
TAAACAGGCGTTACGAAAATTAGAAAATTTCGACCGCACTTGGTATGCTGGGACATTGGGCGTTATGGGCCCCGCGTATG
CGGATTTTTGTGTCACGATACGCTCTGCTTTTATTGAACAGGCAGAAAATGACAGTCAATTATGTGTTTTTGCTGGGGCT
GGGATTGTTGAAGGCTCTATTCCATTGTTAGAATGGCGAGAGATTGAGCGTAAAGCTATGGGCTTAGTGTCGTTATTGCA
ACAGAATCAATTATAA

Upstream 100 bases:

>100_bases
AAGGCGGGAGAAAAGATTTTGTAAAAATTTCTATTAAAGTGCGGTAGAATTAACGCTTATTTTTAACTTATTTTTTTATC
GAAAACAATGCTTTTTCAAT

Downstream 100 bases:

>100_bases
AAGGGAACAGATATGTCAATAAGCGTATTTAATCGTTGTTGGTCAAAAGTGATTTTGGAAACCTTGGTTCGCCAAGGTGT
TGCGCATTTTTGTATTGCAC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MDNLQSIKAQFIAQINAYQPEKDRDIVVFQCNTETTFSLLAWLKAQQYYPQFYLHGRDGATKWASIGQVRQFSDVSAAAH
FIQEQQLALLGGLQFYGDALFVLPRLLLQQRQDGMTITLFIDGKQFEQDKLVALACLSTFEKQTALQTVKQEISLISQKA
DQAEWCRWVEQGLQKIKQGELSKIVLANERCFKTAAPLAATDLLAESEKYNLGCYHFMLAESEQRAFIGSSPELLYRRHG
LQLKTEALAGTAFMGEDEQQNQQQSDWLLHDKKNEYENQLVVDGICQNLQPFVQQITIEKVELKKLRKVQHLRRRISAQL
KAGCGDKDILLAMHPTAAVAGLPQLEAKQALRKLENFDRTWYAGTLGVMGPAYADFCVTIRSAFIEQAENDSQLCVFAGA
GIVEGSIPLLEWREIERKAMGLVSLLQQNQL

Sequences:

>Translated_431_residues
MDNLQSIKAQFIAQINAYQPEKDRDIVVFQCNTETTFSLLAWLKAQQYYPQFYLHGRDGATKWASIGQVRQFSDVSAAAH
FIQEQQLALLGGLQFYGDALFVLPRLLLQQRQDGMTITLFIDGKQFEQDKLVALACLSTFEKQTALQTVKQEISLISQKA
DQAEWCRWVEQGLQKIKQGELSKIVLANERCFKTAAPLAATDLLAESEKYNLGCYHFMLAESEQRAFIGSSPELLYRRHG
LQLKTEALAGTAFMGEDEQQNQQQSDWLLHDKKNEYENQLVVDGICQNLQPFVQQITIEKVELKKLRKVQHLRRRISAQL
KAGCGDKDILLAMHPTAAVAGLPQLEAKQALRKLENFDRTWYAGTLGVMGPAYADFCVTIRSAFIEQAENDSQLCVFAGA
GIVEGSIPLLEWREIERKAMGLVSLLQQNQL
>Mature_431_residues
MDNLQSIKAQFIAQINAYQPEKDRDIVVFQCNTETTFSLLAWLKAQQYYPQFYLHGRDGATKWASIGQVRQFSDVSAAAH
FIQEQQLALLGGLQFYGDALFVLPRLLLQQRQDGMTITLFIDGKQFEQDKLVALACLSTFEKQTALQTVKQEISLISQKA
DQAEWCRWVEQGLQKIKQGELSKIVLANERCFKTAAPLAATDLLAESEKYNLGCYHFMLAESEQRAFIGSSPELLYRRHG
LQLKTEALAGTAFMGEDEQQNQQQSDWLLHDKKNEYENQLVVDGICQNLQPFVQQITIEKVELKKLRKVQHLRRRISAQL
KAGCGDKDILLAMHPTAAVAGLPQLEAKQALRKLENFDRTWYAGTLGVMGPAYADFCVTIRSAFIEQAENDSQLCVFAGA
GIVEGSIPLLEWREIERKAMGLVSLLQQNQL

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family

Homologues:

Organism=Escherichia coli, GI87082077, Length=402, Percent_Identity=38.0597014925373, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786809, Length=282, Percent_Identity=28.7234042553192, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787518, Length=269, Percent_Identity=27.1375464684015, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788114, Length=215, Percent_Identity=26.9767441860465, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENF_PASMU (Q9CPI5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244990.1
- ProteinModelPortal:   Q9CPI5
- SMR:   Q9CPI5
- GeneID:   1243400
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0053
- NMPDR:   fig|272843.1.peg.53
- HOGENOM:   HBG405459
- OMA:   WYAGTLG
- ProtClustDB:   CLSK869946
- BioCyc:   PMUL272843:PM0053-MONOMER
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236:SF3
- PANTHER:   PTHR11236
- TIGRFAMs:   TIGR00543

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 48727; Mature: 48727

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLQSIKAQFIAQINAYQPEKDRDIVVFQCNTETTFSLLAWLKAQQYYPQFYLHGRDGA
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCC
TKWASIGQVRQFSDVSAAAHFIQEQQLALLGGLQFYGDALFVLPRLLLQQRQDGMTITLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IDGKQFEQDKLVALACLSTFEKQTALQTVKQEISLISQKADQAEWCRWVEQGLQKIKQGE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LSKIVLANERCFKTAAPLAATDLLAESEKYNLGCYHFMLAESEQRAFIGSSPELLYRRHG
CCEEEEECCHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCC
LQLKTEALAGTAFMGEDEQQNQQQSDWLLHDKKNEYENQLVVDGICQNLQPFVQQITIEK
CCEEHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VELKKLRKVQHLRRRISAQLKAGCGDKDILLAMHPTAAVAGLPQLEAKQALRKLENFDRT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCC
WYAGTLGVMGPAYADFCVTIRSAFIEQAENDSQLCVFAGAGIVEGSIPLLEWREIERKAM
EECCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHH
GLVSLLQQNQL
HHHHHHHHCCC
>Mature Secondary Structure
MDNLQSIKAQFIAQINAYQPEKDRDIVVFQCNTETTFSLLAWLKAQQYYPQFYLHGRDGA
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCC
TKWASIGQVRQFSDVSAAAHFIQEQQLALLGGLQFYGDALFVLPRLLLQQRQDGMTITLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IDGKQFEQDKLVALACLSTFEKQTALQTVKQEISLISQKADQAEWCRWVEQGLQKIKQGE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LSKIVLANERCFKTAAPLAATDLLAESEKYNLGCYHFMLAESEQRAFIGSSPELLYRRHG
CCEEEEECCHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCC
LQLKTEALAGTAFMGEDEQQNQQQSDWLLHDKKNEYENQLVVDGICQNLQPFVQQITIEK
CCEEHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VELKKLRKVQHLRRRISAQLKAGCGDKDILLAMHPTAAVAGLPQLEAKQALRKLENFDRT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCC
WYAGTLGVMGPAYADFCVTIRSAFIEQAENDSQLCVFAGAGIVEGSIPLLEWREIERKAM
EECCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHH
GLVSLLQQNQL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100