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The map label for this gene is mltF

Identifier: 15601910

GI number: 15601910

Start: 48229

End: 49647

Strand: Direct

Name: mltF

Synonym: PM0045

Alternate gene names: 15601910

Gene position: 48229-49647 (Clockwise)

Preceding gene: 15601907

Following gene: 15601913

Centisome position: 2.14

GC content: 40.87

Gene sequence:

>1419_bases
ATGGTGTTTCCTTGGCAACAAATCATGCGTTCTGAAGAGAACCGCTATACTGCGATTCAAAGTCGCGGTAAGTTGGTGGT
GGGAACAATTAATAATCCGATTTCTTATTTTATTGATCATGCAGGACAAGCGGCGGGACTTGAGTATGAGTTGAGTAAGG
CGTTTGCTGACTATCTTAATGTGGATCTCGAAATTCTCGCGATGTCAAATGGTGATGAACTCTTTAGTGCGCTGAAAAAT
AATAAAATTGATATTGCGGCAGCGAATTTGTTGTATCAACCGAATAAATTAGATATGTTCCAAGTAGGTCCCACTTATAA
TTCGGCTTCGTGGCAGTTAGTGTACCGTAAGGGAACAGAACGTCCACGTAGTCTAGGGGAATTGAAAGGCAGTTTAAGTA
TTGCCTCTGGATCAGAGCTCACGGAATTATTAAAAGTAGAAAAAGAAAAATACCCTGACTTAATCTGGCGTGTAAATGAA
AAACTTACTCCGGAAGAACTGCTGATTCAAATGGCAGATGGCAAAATTGACTATGTGATCGCGAATTCTATTGATGTGTC
TGCGGTGCAACAAATTAAGCCAAATGTAGCGGTCGCGTTTGATGTGACGGATGAATCGACGGTGCATTGGTATTTACCAA
GTAATTCGTATAGTGAATTGCAAGCCTCATTATTAGATTTTATGAATAGCGCTATTGAAACAGGCTTGATTGCGCGTATT
GAAGAAAAATATTTTAATCATTTAGCGCAATTTGACTATGTTGATACCCGTAGCTATCTACGGGCAATAGAAACGGTTTT
ACCGAAATTTGCCCCGCTCTTTGAAAAATATAAAGGCGATCTTGATTGGTGTTTATTGGCGGCAATTGCTTATCAAGAGT
CCCATTGGAATCCTGATGCAACCTCACCGACCGGGGTACGAGGTATGATGATGTTAACGAAAGCCACAGCAGAGCGGATG
CGAATTCAAGACAGAACGGATCCGGAACAAAGTATTAAAGCCGGTTCAGAATATTTACATTGGCTTATTACGCAAATGCC
TGATACGATTAAAGAAGATGAGCGTATTTGGTTTGCCTTAGCGGCGTATAATATGGGATTAGGGCATTTGTTAGATGCGC
GCCGTTTAACCAAGAGCCTAGGTGGTGATCCCGATAATTGGTTAGATGTGAAGAAGAATTTGCCACTATTAGCAGAAAAA
CGCTATTACACGGGGTTAAAATACGGGTATGCGCGAGGCTATGAAGCATATCACTATGTGGAAAATATCCGTCGTTATAT
GAACAGCATTGTGCATTATTATCGGGTACAGCAAGCACAAATTGAAGAAACATTAGATACACAGGCCGCCGAAATAGAAA
ACATCAACGAAATCAAGAAAGAAACGGATACGTTAGCGACAACCGTAACGACACAATAA

Upstream 100 bases:

>100_bases
AAATGCGTCAATTTTCAGTTATCGAGTCAATAGAGGTCATTTGAAAGGTTTATTTCTACGTATTATTGCGATTGTAGCCT
TATTGCTATGGGCAATAGAT

Downstream 100 bases:

>100_bases
GAGGAAGAGTATGGCGAGACGTAAAAGCTTTTTGAAGCCGACAAAAAAATTATATCGTTTATCACAAGGACGCGCATTAC
GTTTAAAACGCTTAAAGCAG

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVFPWQQIMRSEENRYTAIQSRGKLVVGTINNPISYFIDHAGQAAGLEYELSKAFADYLNVDLEILAMSNGDELFSALKN
NKIDIAAANLLYQPNKLDMFQVGPTYNSASWQLVYRKGTERPRSLGELKGSLSIASGSELTELLKVEKEKYPDLIWRVNE
KLTPEELLIQMADGKIDYVIANSIDVSAVQQIKPNVAVAFDVTDESTVHWYLPSNSYSELQASLLDFMNSAIETGLIARI
EEKYFNHLAQFDYVDTRSYLRAIETVLPKFAPLFEKYKGDLDWCLLAAIAYQESHWNPDATSPTGVRGMMMLTKATAERM
RIQDRTDPEQSIKAGSEYLHWLITQMPDTIKEDERIWFALAAYNMGLGHLLDARRLTKSLGGDPDNWLDVKKNLPLLAEK
RYYTGLKYGYARGYEAYHYVENIRRYMNSIVHYYRVQQAQIEETLDTQAAEIENINEIKKETDTLATTVTTQ

Sequences:

>Translated_472_residues
MVFPWQQIMRSEENRYTAIQSRGKLVVGTINNPISYFIDHAGQAAGLEYELSKAFADYLNVDLEILAMSNGDELFSALKN
NKIDIAAANLLYQPNKLDMFQVGPTYNSASWQLVYRKGTERPRSLGELKGSLSIASGSELTELLKVEKEKYPDLIWRVNE
KLTPEELLIQMADGKIDYVIANSIDVSAVQQIKPNVAVAFDVTDESTVHWYLPSNSYSELQASLLDFMNSAIETGLIARI
EEKYFNHLAQFDYVDTRSYLRAIETVLPKFAPLFEKYKGDLDWCLLAAIAYQESHWNPDATSPTGVRGMMMLTKATAERM
RIQDRTDPEQSIKAGSEYLHWLITQMPDTIKEDERIWFALAAYNMGLGHLLDARRLTKSLGGDPDNWLDVKKNLPLLAEK
RYYTGLKYGYARGYEAYHYVENIRRYMNSIVHYYRVQQAQIEETLDTQAAEIENINEIKKETDTLATTVTTQ
>Mature_472_residues
MVFPWQQIMRSEENRYTAIQSRGKLVVGTINNPISYFIDHAGQAAGLEYELSKAFADYLNVDLEILAMSNGDELFSALKN
NKIDIAAANLLYQPNKLDMFQVGPTYNSASWQLVYRKGTERPRSLGELKGSLSIASGSELTELLKVEKEKYPDLIWRVNE
KLTPEELLIQMADGKIDYVIANSIDVSAVQQIKPNVAVAFDVTDESTVHWYLPSNSYSELQASLLDFMNSAIETGLIARI
EEKYFNHLAQFDYVDTRSYLRAIETVLPKFAPLFEKYKGDLDWCLLAAIAYQESHWNPDATSPTGVRGMMMLTKATAERM
RIQDRTDPEQSIKAGSEYLHWLITQMPDTIKEDERIWFALAAYNMGLGHLLDARRLTKSLGGDPDNWLDVKKNLPLLAEK
RYYTGLKYGYARGYEAYHYVENIRRYMNSIVHYYRVQQAQIEETLDTQAAEIENINEIKKETDTLATTVTTQ

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=443, Percent_Identity=51.4672686230248, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_PASMU (Q9CPJ2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244982.1
- ProteinModelPortal:   Q9CPJ2
- GeneID:   1243392
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0045
- NMPDR:   fig|272843.1.peg.45
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   PMUL272843:PM0045-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 53925; Mature: 53925

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFPWQQIMRSEENRYTAIQSRGKLVVGTINNPISYFIDHAGQAAGLEYELSKAFADYLN
CCCCHHHHHCCCCCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
VDLEILAMSNGDELFSALKNNKIDIAAANLLYQPNKLDMFQVGPTYNSASWQLVYRKGTE
CCEEEEEECCCHHHHHHHHCCCEEEEEHHHEECCCCCCEEEECCCCCCCCEEEEEECCCC
RPRSLGELKGSLSIASGSELTELLKVEKEKYPDLIWRVNEKLTPEELLIQMADGKIDYVI
CHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCEEEEE
ANSIDVSAVQQIKPNVAVAFDVTDESTVHWYLPSNSYSELQASLLDFMNSAIETGLIARI
ECCCCHHHHHHCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEKYFNHLAQFDYVDTRSYLRAIETVLPKFAPLFEKYKGDLDWCLLAAIAYQESHWNPDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
TSPTGVRGMMMLTKATAERMRIQDRTDPEQSIKAGSEYLHWLITQMPDTIKEDERIWFAL
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEE
AAYNMGLGHLLDARRLTKSLGGDPDNWLDVKKNLPLLAEKRYYTGLKYGYARGYEAYHYV
EHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCHHHCCCHHHHHCCCHHHHHH
ENIRRYMNSIVHYYRVQQAQIEETLDTQAAEIENINEIKKETDTLATTVTTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MVFPWQQIMRSEENRYTAIQSRGKLVVGTINNPISYFIDHAGQAAGLEYELSKAFADYLN
CCCCHHHHHCCCCCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
VDLEILAMSNGDELFSALKNNKIDIAAANLLYQPNKLDMFQVGPTYNSASWQLVYRKGTE
CCEEEEEECCCHHHHHHHHCCCEEEEEHHHEECCCCCCEEEECCCCCCCCEEEEEECCCC
RPRSLGELKGSLSIASGSELTELLKVEKEKYPDLIWRVNEKLTPEELLIQMADGKIDYVI
CHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCEEEEE
ANSIDVSAVQQIKPNVAVAFDVTDESTVHWYLPSNSYSELQASLLDFMNSAIETGLIARI
ECCCCHHHHHHCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEKYFNHLAQFDYVDTRSYLRAIETVLPKFAPLFEKYKGDLDWCLLAAIAYQESHWNPDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
TSPTGVRGMMMLTKATAERMRIQDRTDPEQSIKAGSEYLHWLITQMPDTIKEDERIWFAL
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEE
AAYNMGLGHLLDARRLTKSLGGDPDNWLDVKKNLPLLAEKRYYTGLKYGYARGYEAYHYV
EHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCHHHCCCHHHHHCCCHHHHHH
ENIRRYMNSIVHYYRVQQAQIEETLDTQAAEIENINEIKKETDTLATTVTTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100