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The map label for this gene is nrfD [H]

Identifier: 15601891

GI number: 15601891

Start: 22510

End: 23469

Strand: Direct

Name: nrfD [H]

Synonym: PM0026

Alternate gene names: 15601891

Gene position: 22510-23469 (Clockwise)

Preceding gene: 15601890

Following gene: 15601892

Centisome position: 1.0

GC content: 41.56

Gene sequence:

>960_bases
ATGAACAGTCCATTTCATTTCCCGTCTTTAGTATGGGATCATACCATCGCCGTTTATTTGTTCTTATTGGGGATCTCTGC
CGGTGCAACCATGTTAGCCGTTTTGTTAAAACGCGCAGGGCTAGCTGGCGCAGAACCGTCAAACAATCATGTGATACGTT
GTACGGCATTTTTGGCGCCATTAAGTCTGATTATTGGCTTGGTGATTTTGATTTTCCATTTAACTAAGCCATGGACCTTT
TGGTACTTGATGTTTAATTACAGTACCACCTCTATTATGTCGATGGGGGTTATGCTATTCCAGGCCTACATGTTGTTTTT
ACTGGTCTGGTTAGCGATTATTTTCCATCAGCTTGTGGCAACTTTAATTGAGCAATATGCCAAACCGTTACGATTTGTGA
ATAAATTGATTGCTTTTGCTGCCAAATTCACTGGATTAATTGAAATTATTTTGATAATTCTCTCTGTGTTATTGGGCGCC
TATACGGGCTTCTTATTATCGGCTTTGATTAGCTACCCAATGTTGAATAACCCAGTGTTACCAATTTTATTCTTGGCCTC
AGGGACATCCTCAGGTATTGCGGCATTAATTTTCTGTACCTTGCTCTTTACTAAAGAAGACACCCATTCACCTGCGTTAG
GCTTCTTACATAAGTTTGAATTGCCGGTAGTGTTGATGGAAATCTTTTTACTTGCCGCCTTCTTTATTGGCTTGTATTTA
GGGGGCGGTCAAAAAACGGTGGCATTTGAGACCGCACTTTTAGGTGGATTCTGGGCACATGTCTTCTGGATTGGGGTAGT
TGGTATTGGTTTAGTCTTGCCATTACTCCTCAATGGCATAGCCACTCCGCAGTTAAAACATCGCAAAGGCTTTATGCTAC
TGGTGGCTTGCTTAGGCTTAATTGGCGTGTTATGTTTGCGTTTCTTTATCCTCTATGCAGGACAAATGACGCTAGCATAA

Upstream 100 bases:

>100_bases
CACAAAAAGTCAAATCGCAGCAAGTGTATCGGACGAAGGTGGAATTAGGCACCGATCCACAGCTGTATCATGTACCAGCT
AAAAGAGGGGAGCTAAGACG

Downstream 100 bases:

>100_bases
ATGCTTCATTTTATGAGAAGGCGAACCTAAGTTCGCCTTTTTGATACAGCTTTTCAAATTTGTTTATATTCACTATGATC
CCAGAACTTGGTTTTTTGGC

Product: hypothetical protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNSPFHFPSLVWDHTIAVYLFLLGISAGATMLAVLLKRAGLAGAEPSNNHVIRCTAFLAPLSLIIGLVILIFHLTKPWTF
WYLMFNYSTTSIMSMGVMLFQAYMLFLLVWLAIIFHQLVATLIEQYAKPLRFVNKLIAFAAKFTGLIEIILIILSVLLGA
YTGFLLSALISYPMLNNPVLPILFLASGTSSGIAALIFCTLLFTKEDTHSPALGFLHKFELPVVLMEIFLLAAFFIGLYL
GGGQKTVAFETALLGGFWAHVFWIGVVGIGLVLPLLLNGIATPQLKHRKGFMLLVACLGLIGVLCLRFFILYAGQMTLA

Sequences:

>Translated_319_residues
MNSPFHFPSLVWDHTIAVYLFLLGISAGATMLAVLLKRAGLAGAEPSNNHVIRCTAFLAPLSLIIGLVILIFHLTKPWTF
WYLMFNYSTTSIMSMGVMLFQAYMLFLLVWLAIIFHQLVATLIEQYAKPLRFVNKLIAFAAKFTGLIEIILIILSVLLGA
YTGFLLSALISYPMLNNPVLPILFLASGTSSGIAALIFCTLLFTKEDTHSPALGFLHKFELPVVLMEIFLLAAFFIGLYL
GGGQKTVAFETALLGGFWAHVFWIGVVGIGLVLPLLLNGIATPQLKHRKGFMLLVACLGLIGVLCLRFFILYAGQMTLA
>Mature_319_residues
MNSPFHFPSLVWDHTIAVYLFLLGISAGATMLAVLLKRAGLAGAEPSNNHVIRCTAFLAPLSLIIGLVILIFHLTKPWTF
WYLMFNYSTTSIMSMGVMLFQAYMLFLLVWLAIIFHQLVATLIEQYAKPLRFVNKLIAFAAKFTGLIEIILIILSVLLGA
YTGFLLSALISYPMLNNPVLPILFLASGTSSGIAALIFCTLLFTKEDTHSPALGFLHKFELPVVLMEIFLLAAFFIGLYL
GGGQKTVAFETALLGGFWAHVFWIGVVGIGLVLPLLLNGIATPQLKHRKGFMLLVACLGLIGVLCLRFFILYAGQMTLA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=320, Percent_Identity=51.25, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35049; Mature: 35049

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPFHFPSLVWDHTIAVYLFLLGISAGATMLAVLLKRAGLAGAEPSNNHVIRCTAFLAP
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHH
LSLIIGLVILIFHLTKPWTFWYLMFNYSTTSIMSMGVMLFQAYMLFLLVWLAIIFHQLVA
HHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIEQYAKPLRFVNKLIAFAAKFTGLIEIILIILSVLLGAYTGFLLSALISYPMLNNPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
PILFLASGTSSGIAALIFCTLLFTKEDTHSPALGFLHKFELPVVLMEIFLLAAFFIGLYL
HHHEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGQKTVAFETALLGGFWAHVFWIGVVGIGLVLPLLLNGIATPQLKHRKGFMLLVACLGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHH
IGVLCLRFFILYAGQMTLA
HHHHHHHHHHHHHCCHHCC
>Mature Secondary Structure
MNSPFHFPSLVWDHTIAVYLFLLGISAGATMLAVLLKRAGLAGAEPSNNHVIRCTAFLAP
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHH
LSLIIGLVILIFHLTKPWTFWYLMFNYSTTSIMSMGVMLFQAYMLFLLVWLAIIFHQLVA
HHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIEQYAKPLRFVNKLIAFAAKFTGLIEIILIILSVLLGAYTGFLLSALISYPMLNNPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
PILFLASGTSSGIAALIFCTLLFTKEDTHSPALGFLHKFELPVVLMEIFLLAAFFIGLYL
HHHEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGQKTVAFETALLGGFWAHVFWIGVVGIGLVLPLLLNGIATPQLKHRKGFMLLVACLGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHH
IGVLCLRFFILYAGQMTLA
HHHHHHHHHHHHHCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]