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The map label for this gene is nrfB [H]

Identifier: 15601889

GI number: 15601889

Start: 21141

End: 21836

Strand: Direct

Name: nrfB [H]

Synonym: PM0024

Alternate gene names: 15601889

Gene position: 21141-21836 (Clockwise)

Preceding gene: 15601888

Following gene: 15601890

Centisome position: 0.94

GC content: 42.1

Gene sequence:

>696_bases
ATGACGATCAGTGCGGTGCGTTTAACACTACATTTTGAGGTAATAAACATGCGCTTACATTCCCTAATCAAACAAGGAAT
CGCTATCACAGCAGGATTGATTGCGTTTAGTGTGGCGACTTTCGCGCAGGCAGAGAAAGCCAACAGTAACGAAGTCAGTC
AATTAATGTATGAGCCACAATTGGAACATCAACGTGATCCGAACCAATATTGTGCGAAATGCCATAAATTAGAAGCTAAT
GATAGCCAATCTGGTGGGGTGTTCCATTTTGGAAAATTCCACGGAACTCATTTATCGGAAAAAAATCCGAATACCGGTAA
ACCGATTACCTGCGTGAACTGTCATGGCAATATTTCTGAAGATCACCGCCGTGGTGTGAAAGATGTGATGCGTTTCCATG
GCAATATTTTCAGCGATGAAAAACCAATGTTCACCGTAGAGGAACAAAATCAGGTTTGCTTTGCGTGTCACCAACCGAAA
AAATTACGCGAAACACTTTGGGCGCATGATGTACATGCCATGAAGTTACCTTGTGCAAGTTGCCATACTTTGCATCCCGC
ACAAGATAAAATGTCAGGTATTGCGAAGAAAGACCAAGTGAAACTTTGCGTGGATTGTCACGGTGAACAACAAAAACGTA
AGGCAAATTTACAACCACCGCATATTTATGCTCCGCAACAAAAGGATAAACAATGA

Upstream 100 bases:

>100_bases
ACTGTTGTTCCTCAATGGGATAAAGAAGCCCGTGAAAAAGGCTTACTTCCTGCGGAGAAATAAACAGAAACTTTGTCAAA
AATGACCGCACTTTGAGGTT

Downstream 100 bases:

>100_bases
CAGCTTGCTCACGCCGAAACTTTGTTTCCGGCATGGGAGCGTTAATCCTCATGACGGGAACATCAATAAAATCAGTGGGA
CAGAACGCAAGCGAGGCGAA

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTISAVRLTLHFEVINMRLHSLIKQGIAITAGLIAFSVATFAQAEKANSNEVSQLMYEPQLEHQRDPNQYCAKCHKLEAN
DSQSGGVFHFGKFHGTHLSEKNPNTGKPITCVNCHGNISEDHRRGVKDVMRFHGNIFSDEKPMFTVEEQNQVCFACHQPK
KLRETLWAHDVHAMKLPCASCHTLHPAQDKMSGIAKKDQVKLCVDCHGEQQKRKANLQPPHIYAPQQKDKQ

Sequences:

>Translated_231_residues
MTISAVRLTLHFEVINMRLHSLIKQGIAITAGLIAFSVATFAQAEKANSNEVSQLMYEPQLEHQRDPNQYCAKCHKLEAN
DSQSGGVFHFGKFHGTHLSEKNPNTGKPITCVNCHGNISEDHRRGVKDVMRFHGNIFSDEKPMFTVEEQNQVCFACHQPK
KLRETLWAHDVHAMKLPCASCHTLHPAQDKMSGIAKKDQVKLCVDCHGEQQKRKANLQPPHIYAPQQKDKQ
>Mature_230_residues
TISAVRLTLHFEVINMRLHSLIKQGIAITAGLIAFSVATFAQAEKANSNEVSQLMYEPQLEHQRDPNQYCAKCHKLEAND
SQSGGVFHFGKFHGTHLSEKNPNTGKPITCVNCHGNISEDHRRGVKDVMRFHGNIFSDEKPMFTVEEQNQVCFACHQPKK
LRETLWAHDVHAMKLPCASCHTLHPAQDKMSGIAKKDQVKLCVDCHGEQQKRKANLQPPHIYAPQQKDKQ

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=204, Percent_Identity=39.7058823529412, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 26084; Mature: 25953

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAVRLTLHFEVINMRLHSLIKQGIAITAGLIAFSVATFAQAEKANSNEVSQLMYEPQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
LEHQRDPNQYCAKCHKLEANDSQSGGVFHFGKFHGTHLSEKNPNTGKPITCVNCHGNISE
HHCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCH
DHRRGVKDVMRFHGNIFSDEKPMFTVEEQNQVCFACHQPKKLRETLWAHDVHAMKLPCAS
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCHHHCCCCCC
CHTLHPAQDKMSGIAKKDQVKLCVDCHGEQQKRKANLQPPHIYAPQQKDKQ
CCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TISAVRLTLHFEVINMRLHSLIKQGIAITAGLIAFSVATFAQAEKANSNEVSQLMYEPQ
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
LEHQRDPNQYCAKCHKLEANDSQSGGVFHFGKFHGTHLSEKNPNTGKPITCVNCHGNISE
HHCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCH
DHRRGVKDVMRFHGNIFSDEKPMFTVEEQNQVCFACHQPKKLRETLWAHDVHAMKLPCAS
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCHHHCCCCCC
CHTLHPAQDKMSGIAKKDQVKLCVDCHGEQQKRKANLQPPHIYAPQQKDKQ
CCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]