Click here to switch to the map view.

The map label for this gene is nrfA

Identifier: 15601888

GI number: 15601888

Start: 19571

End: 21103

Strand: Direct

Name: nrfA

Synonym: PM0023

Alternate gene names: 15601888

Gene position: 19571-21103 (Clockwise)

Preceding gene: 15601887

Following gene: 15601889

Centisome position: 0.87

GC content: 44.03

Gene sequence:

>1533_bases
CTGGTCGTTTTTTTATTCATTTTATATAGGCAAAGTGATTTAAAGTTTAAATCTATGAATGGAGTGATTATCGTGAACAC
ATTGAAAAAACGCTTATTCGTTGCAACGACAATGATCTGGGGACTCTCAGTGACATTGCCCGTTCTAGCGGAATATAAAC
CGATTGAACAACCGGTTGAGCCAGCAAATCCAAGTTTAAAAATTGAATCGCGTAACGATTTGTTCAAAGAAAAATATCCA
AAGCAATATCAAACTTGGGCGGATACCTCAAAATCGACGGATCTTGATTCGGTTAACTATGAAGATCCTCGCGTGATTGT
ACTATGGGCAGGCTATGCGTTTGCCAAAGATTATAAAAAGCCACGTGGACATTTCTACGCGGTAACCGATGTGAGAGAAA
TTTTACGTACGGCAGCGCCTATGACGCCTGATGCCGGTCCAATGCCAATGGCATGTTGGTCTTGTAAAAGTCCCGATGTG
CCTCGCTTAATTGCTGAGCGCGGTGAAGCGGGTTATTTTGGGGCAACATGGGCAAGTGGTGGCTCAGAAGTCGTGAACCC
GATTGGCTGTGCAGATTGTCATGACACCACCTCAAAAGACTTTGCGGAAGGCAAACCCGCCTTACGTATTGCGCGTCCTT
ACGTGTTACGTGCCTTAGAAAAAATTGATCATAAATTTGATACCTCAGACCGGACAGATCAGCGTGCTGCATTGTGTGCA
AACTGTCATGTGGAATACTATTTCGCGGGTGATTTAAAACAAGTGACGTTCCCATGGGATAATGGTATCACCGTGGACGC
CATGGAGAAATATTACGATGACATCGGTTTTGTGGATTGGACACATGCTGTTTCGAAAGCACCAATGTTAAAAGCACAAC
ACCCTGATTATGAAACCTGGATGCTCGGTGTGCATGGTAAAAATGGAGTGACTTGTATTGACTGCCATATGCCAAAAGTA
CAGGGCGCAGACGGTAAAGTCTATACTGACCATCAAATCGGTAACCCTTTCAATGCCTTTGAACACACTTGTGCAAACTG
CCATGACCAAAGCAAAGAAAAATTACAAGCAATGGTCAAATCACGTAAAACCGAAATTAAAGATGTGATGTTACGTCTTG
AAGATCAATTGGTTGCCGCACACTTTGAAGCGAAAGCAGCTTGGGAGGCCGGCGCGACCAAAGAAGAAATGAAAGAGGCA
TTGCAAGATATCCGTCATGCGCAATGGCGTTGGGATTATGCGGCAGCAGGACATGGCGGACATATTCATGCACCAGATGT
GTTGTTGAAAGTGATTGGGACAGGCTTGGATAAATCCTCTGATGCCAGAACTAAATTAGTGCGTGTCTTAGCCAAACATG
GCATTACCGATCCTGTGCAATTACCAGATATTTCAACGGCAGAAAATGCGTGGAAAGCGACAGGCGTAGATATTGAAAAA
GAACGTAAGGCAAAAGCCGAATTCCTCAAAACTGTTGTTCCTCAATGGGATAAAGAAGCCCGTGAAAAAGGCTTACTTCC
TGCGGAGAAATAA

Upstream 100 bases:

>100_bases
TATGGTTATTTGCTATTTTTCGACATTAAGGAACTCTCTTTTTATCAGCTAGCTAGTTGTTTTTAATGATAAAAATTATC
ATTTAGGATGATGAGCAGGT

Downstream 100 bases:

>100_bases
ACAGAAACTTTGTCAAAAATGACCGCACTTTGAGGTTATGACGATCAGTGCGGTGCGTTTAACACTACATTTTGAGGTAA
TAAACATGCGCTTACATTCC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVVFLFILYRQSDLKFKSMNGVIIVNTLKKRLFVATTMIWGLSVTLPVLAEYKPIEQPVEPANPSLKIESRNDLFKEKYP
KQYQTWADTSKSTDLDSVNYEDPRVIVLWAGYAFAKDYKKPRGHFYAVTDVREILRTAAPMTPDAGPMPMACWSCKSPDV
PRLIAERGEAGYFGATWASGGSEVVNPIGCADCHDTTSKDFAEGKPALRIARPYVLRALEKIDHKFDTSDRTDQRAALCA
NCHVEYYFAGDLKQVTFPWDNGITVDAMEKYYDDIGFVDWTHAVSKAPMLKAQHPDYETWMLGVHGKNGVTCIDCHMPKV
QGADGKVYTDHQIGNPFNAFEHTCANCHDQSKEKLQAMVKSRKTEIKDVMLRLEDQLVAAHFEAKAAWEAGATKEEMKEA
LQDIRHAQWRWDYAAAGHGGHIHAPDVLLKVIGTGLDKSSDARTKLVRVLAKHGITDPVQLPDISTAENAWKATGVDIEK
ERKAKAEFLKTVVPQWDKEAREKGLLPAEK

Sequences:

>Translated_510_residues
MVVFLFILYRQSDLKFKSMNGVIIVNTLKKRLFVATTMIWGLSVTLPVLAEYKPIEQPVEPANPSLKIESRNDLFKEKYP
KQYQTWADTSKSTDLDSVNYEDPRVIVLWAGYAFAKDYKKPRGHFYAVTDVREILRTAAPMTPDAGPMPMACWSCKSPDV
PRLIAERGEAGYFGATWASGGSEVVNPIGCADCHDTTSKDFAEGKPALRIARPYVLRALEKIDHKFDTSDRTDQRAALCA
NCHVEYYFAGDLKQVTFPWDNGITVDAMEKYYDDIGFVDWTHAVSKAPMLKAQHPDYETWMLGVHGKNGVTCIDCHMPKV
QGADGKVYTDHQIGNPFNAFEHTCANCHDQSKEKLQAMVKSRKTEIKDVMLRLEDQLVAAHFEAKAAWEAGATKEEMKEA
LQDIRHAQWRWDYAAAGHGGHIHAPDVLLKVIGTGLDKSSDARTKLVRVLAKHGITDPVQLPDISTAENAWKATGVDIEK
ERKAKAEFLKTVVPQWDKEAREKGLLPAEK
>Mature_510_residues
MVVFLFILYRQSDLKFKSMNGVIIVNTLKKRLFVATTMIWGLSVTLPVLAEYKPIEQPVEPANPSLKIESRNDLFKEKYP
KQYQTWADTSKSTDLDSVNYEDPRVIVLWAGYAFAKDYKKPRGHFYAVTDVREILRTAAPMTPDAGPMPMACWSCKSPDV
PRLIAERGEAGYFGATWASGGSEVVNPIGCADCHDTTSKDFAEGKPALRIARPYVLRALEKIDHKFDTSDRTDQRAALCA
NCHVEYYFAGDLKQVTFPWDNGITVDAMEKYYDDIGFVDWTHAVSKAPMLKAQHPDYETWMLGVHGKNGVTCIDCHMPKV
QGADGKVYTDHQIGNPFNAFEHTCANCHDQSKEKLQAMVKSRKTEIKDVMLRLEDQLVAAHFEAKAAWEAGATKEEMKEA
LQDIRHAQWRWDYAAAGHGGHIHAPDVLLKVIGTGLDKSSDARTKLVRVLAKHGITDPVQLPDISTAENAWKATGVDIEK
ERKAKAEFLKTVVPQWDKEAREKGLLPAEK

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=451, Percent_Identity=58.0931263858093, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_PASMU (Q9CPL4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244960.1
- ProteinModelPortal:   Q9CPL4
- SMR:   Q9CPL4
- GeneID:   1243370
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0023
- NMPDR:   fig|272843.1.peg.23
- HOGENOM:   HBG488281
- OMA:   PWDMGTT
- ProtClustDB:   PRK11125
- BioCyc:   PMUL272843:PM0023-MONOMER
- BRENDA:   1.7.2.2
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243
- TIGRFAMs:   TIGR03152

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 57261; Mature: 57261

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 152-152 BINDING 155-155 BINDING 190-190 BINDING 193-193 BINDING 239-239 BINDING 242-242 BINDING 246-246 BINDING 294-294 BINDING 312-312 BINDING 315-315 BINDING 344-344 BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVFLFILYRQSDLKFKSMNGVIIVNTLKKRLFVATTMIWGLSVTLPVLAEYKPIEQPVE
CEEEEEEEECCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PANPSLKIESRNDLFKEKYPKQYQTWADTSKSTDLDSVNYEDPRVIVLWAGYAFAKDYKK
CCCCCEEECCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCC
PRGHFYAVTDVREILRTAAPMTPDAGPMPMACWSCKSPDVPRLIAERGEAGYFGATWASG
CCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCCCCC
GSEVVNPIGCADCHDTTSKDFAEGKPALRIARPYVLRALEKIDHKFDTSDRTDQRAALCA
CHHHCCCCCCCCCCCCCCCHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NCHVEYYFAGDLKQVTFPWDNGITVDAMEKYYDDIGFVDWTHAVSKAPMLKAQHPDYETW
CCEEEEEEECCCEEEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEE
MLGVHGKNGVTCIDCHMPKVQGADGKVYTDHQIGNPFNAFEHTCANCHDQSKEKLQAMVK
EEEEECCCCCEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
SRKTEIKDVMLRLEDQLVAAHFEAKAAWEAGATKEEMKEALQDIRHAQWRWDYAAAGHGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
HIHAPDVLLKVIGTGLDKSSDARTKLVRVLAKHGITDPVQLPDISTAENAWKATGVDIEK
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHCCCCHHH
ERKAKAEFLKTVVPQWDKEAREKGLLPAEK
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MVVFLFILYRQSDLKFKSMNGVIIVNTLKKRLFVATTMIWGLSVTLPVLAEYKPIEQPVE
CEEEEEEEECCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PANPSLKIESRNDLFKEKYPKQYQTWADTSKSTDLDSVNYEDPRVIVLWAGYAFAKDYKK
CCCCCEEECCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCC
PRGHFYAVTDVREILRTAAPMTPDAGPMPMACWSCKSPDVPRLIAERGEAGYFGATWASG
CCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCCCCC
GSEVVNPIGCADCHDTTSKDFAEGKPALRIARPYVLRALEKIDHKFDTSDRTDQRAALCA
CHHHCCCCCCCCCCCCCCCHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NCHVEYYFAGDLKQVTFPWDNGITVDAMEKYYDDIGFVDWTHAVSKAPMLKAQHPDYETW
CCEEEEEEECCCEEEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEE
MLGVHGKNGVTCIDCHMPKVQGADGKVYTDHQIGNPFNAFEHTCANCHDQSKEKLQAMVK
EEEEECCCCCEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
SRKTEIKDVMLRLEDQLVAAHFEAKAAWEAGATKEEMKEALQDIRHAQWRWDYAAAGHGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
HIHAPDVLLKVIGTGLDKSSDARTKLVRVLAKHGITDPVQLPDISTAENAWKATGVDIEK
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHCCCCHHH
ERKAKAEFLKTVVPQWDKEAREKGLLPAEK
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100