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The map label for this gene is purN [H]

Identifier: 15601885

GI number: 15601885

Start: 16766

End: 17407

Strand: Reverse

Name: purN [H]

Synonym: PM0020

Alternate gene names: 15601885

Gene position: 17407-16766 (Counterclockwise)

Preceding gene: 15601886

Following gene: 15601884

Centisome position: 0.77

GC content: 42.83

Gene sequence:

>642_bases
ATGAAAAAAATTGTTGTTTTAGTGTCAGGACATGGCAGTAATTTACAGGCATTAATCGATGCCTGTCATAGCGGACAGAT
TGCAGGTAAAATCGTTGCTGTCATCAGTAATCAAGCGGAGGCTTATGCCTTAGAGCGAGCTCAAAGCGCGTCCATTCCAA
GTAAAGTGTTTTTACGCAAAGATTTTGCTAATAACCGTGCTATGGATGAGCAAATTGGTCATTATATTGAATCTGTCCAA
GCCGATTTGATCGTACTCGCAGGCTATATGAAAATTTTAAGTCCTGCGTTTACTCAACGTTTTGCTGGCAAAATTCTCAA
TATTCATCCTTCGCTTCTCCCTAAATACCCGGGTCTCAATACTTATCAGCAAGCCCTCGATGCAGGTGAGCGCGAACATG
GTACCTCAGTCCATTTTGTGAATGAAGAGGTGGATGCAGGTGCCGTCATTTTACAAGCGAAAGTGCCTATCTTTGCTGAA
GACCGTATTGAAGACATTGAGCAACGTGTCAAAGCGCAAGAGTTACGCATTTATCCTTTAGTCGTCAAATGGTTTGTAGA
GGAGCGTTTAACGTTGATCGGTGAACATGCTTTCCTTGATGGCAAACAATTGCCCCCACAAGGGTATGCGATGGAAGAAT
AA

Upstream 100 bases:

>100_bases
ATACGGCACTTGGCTTATTAAAACAAACAGGTGAAAAAGCTTGGGTCATTGGTCAAATTGAACACGCCACTGATGGCGAA
GAACAAGTGATTATTCGCTA

Downstream 100 bases:

>100_bases
ATTGTTTAAAAAAAGTGCGGTCTGTTTTCAGACCGTTTTTTTATGCTTAAAAAATATAAAATTAGCCAAGTCTCTAACTA
TCAATTTTTTAGATTATGTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRKDFANNRAMDEQIGHYIESVQ
ADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLNTYQQALDAGEREHGTSVHFVNEEVDAGAVILQAKVPIFAE
DRIEDIEQRVKAQELRIYPLVVKWFVEERLTLIGEHAFLDGKQLPPQGYAMEE

Sequences:

>Translated_213_residues
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRKDFANNRAMDEQIGHYIESVQ
ADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLNTYQQALDAGEREHGTSVHFVNEEVDAGAVILQAKVPIFAE
DRIEDIEQRVKAQELRIYPLVVKWFVEERLTLIGEHAFLDGKQLPPQGYAMEE
>Mature_213_residues
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRKDFANNRAMDEQIGHYIESVQ
ADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLNTYQQALDAGEREHGTSVHFVNEEVDAGAVILQAKVPIFAE
DRIEDIEQRVKAQELRIYPLVVKWFVEERLTLIGEHAFLDGKQLPPQGYAMEE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=44.6327683615819, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=44.6327683615819, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=44.6327683615819, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788846, Length=211, Percent_Identity=55.9241706161137, Blast_Score=253, Evalue=6e-69,
Organism=Escherichia coli, GI1787483, Length=181, Percent_Identity=32.0441988950276, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=37.2881355932203, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=36.0406091370558, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=46.524064171123, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23605; Mature: 23605

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRK
CCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHEEHHCCHHHHHHHHHHHCCCCHHHHHHH
DFANNRAMDEQIGHYIESVQADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCC
TYQQALDAGEREHGTSVHFVNEEVDAGAVILQAKVPIFAEDRIEDIEQRVKAQELRIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
VVKWFVEERLTLIGEHAFLDGKQLPPQGYAMEE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRK
CCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHEEHHCCHHHHHHHHHHHCCCCHHHHHHH
DFANNRAMDEQIGHYIESVQADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCC
TYQQALDAGEREHGTSVHFVNEEVDAGAVILQAKVPIFAEDRIEDIEQRVKAQELRIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
VVKWFVEERLTLIGEHAFLDGKQLPPQGYAMEE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]