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The map label for this gene is bcr_1 [H]

Identifier: 15601869

GI number: 15601869

Start: 4170

End: 5366

Strand: Direct

Name: bcr_1 [H]

Synonym: PM0004

Alternate gene names: 15601869

Gene position: 4170-5366 (Clockwise)

Preceding gene: 15601868

Following gene: 15601870

Centisome position: 0.18

GC content: 41.77

Gene sequence:

>1197_bases
TTGAATCAACATAAACGCAGTACAGTCAGTGGGCTGTTCATTATGACATTAGCCCTTTTATCCATGTTACCGCCATTGGG
CGTGGATATGTATTTACCCGCATTCTTATTGATCGCTCAGGATTTATCGATTACACCTGAACAAGTTCAACACACCTTAA
CGCTTTTTGCCTTGGGTATGGCTGTGGGGCAATTGTTTTGGGGACCTTTTGGGGATAGTTATGGTCGCAAACCGATTATT
TTGCTCGGTGTGAGTATCAGTGCGATTGCTGCGTTGATGTTAACTGCTACTTATTCTATTGGGCACTTTACCACTTTGCG
CTTTTTACAAGGTTTCTTTGGTGCAGCCCCTGTTGTGCTGGTTGGGGCAATTTTGCGTGATTTATTTCATAAAAATGAAT
TGTCTAAAATCATGTCAACTATTACGTTGGTATTTATGATCGCACCACTTTTAGCCCCTATTATTGGTGGTTATCTGGTG
TTGTGGTTTCATTGGCATACGATTTTCTATGTCATTGCGGTAATGGGGATCTTGTGTTGCCTCTTAACCTTTTTTGTTGT
GCCAGAAACGCACCAAAAAGGGAATCGACAACCCTTACACTTACGGATTATTAGCCGAAACTTTTTCAGTTTATGGCAAC
AAAAAGAAGTTTTAGGTTATATGTTTTCCTCCGCGTTTGGTTTTGGTGGACTGTTTGCTTTTATTACCGCAGGGTCGATT
GTATATATCGGATTATACGGTGTCGCTGTGGAAAATTTTGGCTATTTCTTCATGCTAAATATTGGTGTCATGATTTTCAG
TTCTTTTTTAAATCGCAGATGGGTGACAAAACTGGGAGCAGAAACGATGTTACGTTTGGGGCTCAGTGTACAGTTTATTG
CGGCGGTTGCCTTGGTGTGCATTTCACTTTTTGACCTCGGTTTTTGGCCAATGGCGTTGTGTTGTGCTATTTTTGTCGGG
CAAAATCCCTTGATCTCCTCGAATGCGATGGCATCAATTTTAGAAAAATTTCCGACCATTGCCGGTACCGCAAACTCAAT
GGTGGGAAGCGTCCGTTTTGGTACTGCCTCCCTCGTTGGTTCTTTAGTGGCTGTAATGGAAATGAAAACGGCAGCACCCA
TGTTATTTACCATGGCATTTTGTAGTGTCATGGCAATTGGGTGTTATTACTTTTTGACTTATCGCTCGCTGAAATAA

Upstream 100 bases:

>100_bases
AAAATCGGTCAGATTGAACTCGATGATCGGTTAGCAGAAGGAGAATACCGTTCTTTATCTGCCGAAGAAATTGCAACATT
TAATAAATAAGGATAAGTAT

Downstream 100 bases:

>100_bases
CGTTCGCTTTTTCTCCTTTTATTTAATTTACCTATCAAAGAAATAGGTCATTTTTTAGTTAAAAATCACAAGATTTGTTC
TAAATCAATAAACCCTATAA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNQHKRSTVSGLFIMTLALLSMLPPLGVDMYLPAFLLIAQDLSITPEQVQHTLTLFALGMAVGQLFWGPFGDSYGRKPII
LLGVSISAIAALMLTATYSIGHFTTLRFLQGFFGAAPVVLVGAILRDLFHKNELSKIMSTITLVFMIAPLLAPIIGGYLV
LWFHWHTIFYVIAVMGILCCLLTFFVVPETHQKGNRQPLHLRIISRNFFSLWQQKEVLGYMFSSAFGFGGLFAFITAGSI
VYIGLYGVAVENFGYFFMLNIGVMIFSSFLNRRWVTKLGAETMLRLGLSVQFIAAVALVCISLFDLGFWPMALCCAIFVG
QNPLISSNAMASILEKFPTIAGTANSMVGSVRFGTASLVGSLVAVMEMKTAAPMLFTMAFCSVMAIGCYYFLTYRSLK

Sequences:

>Translated_398_residues
MNQHKRSTVSGLFIMTLALLSMLPPLGVDMYLPAFLLIAQDLSITPEQVQHTLTLFALGMAVGQLFWGPFGDSYGRKPII
LLGVSISAIAALMLTATYSIGHFTTLRFLQGFFGAAPVVLVGAILRDLFHKNELSKIMSTITLVFMIAPLLAPIIGGYLV
LWFHWHTIFYVIAVMGILCCLLTFFVVPETHQKGNRQPLHLRIISRNFFSLWQQKEVLGYMFSSAFGFGGLFAFITAGSI
VYIGLYGVAVENFGYFFMLNIGVMIFSSFLNRRWVTKLGAETMLRLGLSVQFIAAVALVCISLFDLGFWPMALCCAIFVG
QNPLISSNAMASILEKFPTIAGTANSMVGSVRFGTASLVGSLVAVMEMKTAAPMLFTMAFCSVMAIGCYYFLTYRSLK
>Mature_398_residues
MNQHKRSTVSGLFIMTLALLSMLPPLGVDMYLPAFLLIAQDLSITPEQVQHTLTLFALGMAVGQLFWGPFGDSYGRKPII
LLGVSISAIAALMLTATYSIGHFTTLRFLQGFFGAAPVVLVGAILRDLFHKNELSKIMSTITLVFMIAPLLAPIIGGYLV
LWFHWHTIFYVIAVMGILCCLLTFFVVPETHQKGNRQPLHLRIISRNFFSLWQQKEVLGYMFSSAFGFGGLFAFITAGSI
VYIGLYGVAVENFGYFFMLNIGVMIFSSFLNRRWVTKLGAETMLRLGLSVQFIAAVALVCISLFDLGFWPMALCCAIFVG
QNPLISSNAMASILEKFPTIAGTANSMVGSVRFGTASLVGSLVAVMEMKTAAPMLFTMAFCSVMAIGCYYFLTYRSLK

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=386, Percent_Identity=42.7461139896373, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI48994889, Length=368, Percent_Identity=28.5326086956522, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=34.3023255813954, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082312, Length=346, Percent_Identity=26.3005780346821, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790794, Length=305, Percent_Identity=21.6393442622951, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787065, Length=281, Percent_Identity=24.9110320284698, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790195, Length=181, Percent_Identity=29.2817679558011, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=193, Percent_Identity=29.0155440414508, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=184, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324847, Length=308, Percent_Identity=26.2987012987013, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=194, Percent_Identity=24.2268041237113, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43763; Mature: 43763

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]