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The map label for this gene is rsuA_1

Identifier: 15601868

GI number: 15601868

Start: 3461

End: 4159

Strand: Direct

Name: rsuA_1

Synonym: PM0003

Alternate gene names: 15601868

Gene position: 3461-4159 (Clockwise)

Preceding gene: NA

Following gene: 15601869

Centisome position: 0.15

GC content: 41.34

Gene sequence:

>699_bases
ATGCGATTAGATAAATTTTTAGCCGAAAATACAGGACTAACCCGCTCACAAGCGAATAAAGTCTTAAAGCAAAGTGCGGT
CACGGTAAATGGACACGTTGAGAAAAATGGTGCACAAAAAGTCAGTCAAACAGATGAAATTTGTTTAGAAGGCGAGCATT
TACCTTGGGTATCTGCAGGACAATATTTGATGTTATACAAGCCGCAAGGCTATGTTTGCTCTCATGAGGATGGGGATTAT
CCAACGATTTATCAATTCTTTGATTATCCGTTGGCAGGTAAATTGCATAGTGCTGGACGATTAGATGTGGACACGACCGG
GCTCGTGTTATTAACTGATGATGGAAAATGGTCACATCGTATTACGTCCCCAAAACACCATTGTGAGAAAACCTATCTGG
TGACACTTGCCGATCCTGTGGAAAGCCATTATCAACAAGCCTGTGCAGAAGGTATGCTATTGCGTGGTGAGAAAACCCCA
ACGAAGCCTGCAACGTTAGAAATTCTTGATGATTATAATGTGAATTTAACGATTAGTGAAGGACGTTATCATCAAGTCAA
ACGGATGTTTGCGGCGTTGGGGAATAAAGTAGTGGGCTTACACCGCTGGAAAATCGGTCAGATTGAACTCGATGATCGGT
TAGCAGAAGGAGAATACCGTTCTTTATCTGCCGAAGAAATTGCAACATTTAATAAATAA

Upstream 100 bases:

>100_bases
GTTTTTTTCGTCAACTTGCTTATTTTTTAATAATTCCCCCTCTTGTGCTGAAACATAGTAGAATGCGTAAGTTAATGAAT
GAAGGACAACGTAAATTAGC

Downstream 100 bases:

>100_bases
GGATAAGTATTTGAATCAACATAAACGCAGTACAGTCAGTGGGCTGTTCATTATGACATTAGCCCTTTTATCCATGTTAC
CGCCATTGGGCGTGGATATG

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAGQYLMLYKPQGYVCSHEDGDY
PTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTP
TKPATLEILDDYNVNLTISEGRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK

Sequences:

>Translated_232_residues
MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAGQYLMLYKPQGYVCSHEDGDY
PTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTP
TKPATLEILDDYNVNLTISEGRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK
>Mature_232_residues
MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAGQYLMLYKPQGYVCSHEDGDY
PTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTP
TKPATLEILDDYNVNLTISEGRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=56.7685589519651, Blast_Score=265, Evalue=1e-72,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081838, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSUA_PASMU (Q9CPN4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244940.1
- ProteinModelPortal:   Q9CPN4
- SMR:   Q9CPN4
- GeneID:   1243350
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0003
- NMPDR:   fig|272843.1.peg.3
- HOGENOM:   HBG754478
- OMA:   GLHRWKI
- ProtClustDB:   CLSK869926
- BioCyc:   PMUL272843:PM0003-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26096; Mature: 26096

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAG
CCHHHHHHHCCCCCHHHHHHHHHHHCEEEECEECCCCHHHHCCCCHHEECCCCCCEECCC
QYLMLYKPQGYVCSHEDGDYPTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHR
CEEEEECCCCEEECCCCCCCCEEHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTPTKPATLEILDDYNVNLTISE
CCCCHHHCCEEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCEEEEECC
GRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK
CHHHHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCCHHCCCHHHHHCCCC
>Mature Secondary Structure
MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAG
CCHHHHHHHCCCCCHHHHHHHHHHHCEEEECEECCCCHHHHCCCCHHEECCCCCCEECCC
QYLMLYKPQGYVCSHEDGDYPTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHR
CEEEEECCCCEEECCCCCCCCEEHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTPTKPATLEILDDYNVNLTISE
CCCCHHHCCEEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCEEEEECC
GRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK
CHHHHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCCHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100