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The map label for this gene is dut

Identifier: 161579045

GI number: 161579045

Start: 676014

End: 676451

Strand: Reverse

Name: dut

Synonym: TC0565

Alternate gene names: 161579045

Gene position: 676451-676014 (Counterclockwise)

Preceding gene: 15835183

Following gene: 15835181

Centisome position: 63.05

GC content: 45.89

Gene sequence:

>438_bases
ATGAAATTTTTCTGTAAATTAGAGTCGGGTAGCTCTTTACCTGAATACGCTACTTCCGGGGCTTCTGGTGCTGATGTCCG
CGCAAATATTAGTGAGCCAATAGCCATTTTACCTGGACAACGAGCTTTAATTCCTACTGGGATTTCTGTGGATATCCCCC
ACGGGTATGAGATTCAAGTGCGTTCTCGTAGCGGGTTGGCCGTCAAGTATGGCGTTATAGTTTTGCAGTCTCCAGGAACT
GTAGATGCGGATTATAGAGGAGAAATCCGAGTTATTTTAGCTAATCTTGGGGAGTCCACATTTATTGTTGAACCAGGGAT
GCGCATAGCACAACTTGTTGTGGCAAAAGTTGAACAGGTATCTTTTGTCGAAACTCAGGAAGAGTTGACTGCCACAGCGC
GGGGAACCGGTGGATTTGGGCATACTGGGGAATGTTAA

Upstream 100 bases:

>100_bases
CCTCTAGAAGTAGCTCTAAGACCTTTTAGGGGATTTTTTTTGTTGACAGATGACAAGCGAATAAAAGATCATGGGCGGCT
CTTCAAAAATAGGGCCTTCT

Downstream 100 bases:

>100_bases
GATGTCTTGCATTTCCGACCATGAGCCAGCTTTTACTCTAAGTTCATTACTCTCATCGGATTTAGTCGCTTTTTTGCATT
CAGATACTCGGGAAGACATT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKFFCKLESGSSLPEYATSGASGADVRANISEPIAILPGQRALIPTGISVDIPHGYEIQVRSRSGLAVKYGVIVLQSPGT
VDADYRGEIRVILANLGESTFIVEPGMRIAQLVVAKVEQVSFVETQEELTATARGTGGFGHTGEC

Sequences:

>Translated_145_residues
MKFFCKLESGSSLPEYATSGASGADVRANISEPIAILPGQRALIPTGISVDIPHGYEIQVRSRSGLAVKYGVIVLQSPGT
VDADYRGEIRVILANLGESTFIVEPGMRIAQLVVAKVEQVSFVETQEELTATARGTGGFGHTGEC
>Mature_145_residues
MKFFCKLESGSSLPEYATSGASGADVRANISEPIAILPGQRALIPTGISVDIPHGYEIQVRSRSGLAVKYGVIVLQSPGT
VDADYRGEIRVILANLGESTFIVEPGMRIAQLVVAKVEQVSFVETQEELTATARGTGGFGHTGEC

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=141, Percent_Identity=36.1702127659575, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI4503423, Length=141, Percent_Identity=36.1702127659575, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=36.3636363636364, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790071, Length=132, Percent_Identity=39.3939393939394, Blast_Score=107, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=141, Percent_Identity=39.0070921985816, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319729, Length=141, Percent_Identity=36.1702127659575, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921126, Length=141, Percent_Identity=35.4609929078014, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583610, Length=141, Percent_Identity=35.4609929078014, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CHLMU (Q9PKA2)

Other databases:

- EMBL:   AE002160
- PIR:   G81687
- RefSeq:   NP_296941.2
- ProteinModelPortal:   Q9PKA2
- SMR:   Q9PKA2
- GeneID:   1245924
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0565
- TIGR:   TC_0565
- HOGENOM:   HBG436079
- PhylomeDB:   Q9PKA2
- ProtClustDB:   PRK00601
- BioCyc:   CMUR243161:TC_0565-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15325; Mature: 15325

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFCKLESGSSLPEYATSGASGADVRANISEPIAILPGQRALIPTGISVDIPHGYEIQV
CEEEEEECCCCCCCCHHCCCCCCCEEEECCCCCEEECCCCCEEEECCCEEECCCCEEEEE
RSRSGLAVKYGVIVLQSPGTVDADYRGEIRVILANLGESTFIVEPGMRIAQLVVAKVEQV
ECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHH
SFVETQEELTATARGTGGFGHTGEC
HHHHCHHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKFFCKLESGSSLPEYATSGASGADVRANISEPIAILPGQRALIPTGISVDIPHGYEIQV
CEEEEEECCCCCCCCHHCCCCCCCEEEECCCCCEEECCCCCEEEECCCEEECCCCEEEEE
RSRSGLAVKYGVIVLQSPGTVDADYRGEIRVILANLGESTFIVEPGMRIAQLVVAKVEQV
ECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHH
SFVETQEELTATARGTGGFGHTGEC
HHHHCHHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935