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The map label for this gene is nfo

Identifier: 15835527

GI number: 15835527

Start: 1065425

End: 1066291

Strand: Direct

Name: nfo

Synonym: TC0914

Alternate gene names: 15835527

Gene position: 1065425-1066291 (Clockwise)

Preceding gene: 15835524

Following gene: 15835529

Centisome position: 99.3

GC content: 43.83

Gene sequence:

>867_bases
ATGTTTGTACTCCCCCCCCCACAAGAACCTCTTTTAGGAGCCCACACGTCAGCTGCTGGAGGACTCCATAACGCGCTTTA
TGAAGGACGAGACATCGGAGCTACCACTATTCAGCTGTTTACTGCCAACCAACGCCAGTGGAAAAGGCGTTCTCTAACTC
AGAATATGATTGAACAATTCCAAACAGCTTTAGATGAGACCTCTTTGTCCTACATAATGAGTCATGCAGGATATTTAAAT
AATCCTGGAGCTCCCAATCCTGAGATACTAGAGAAGACGCGCATTTGCATGTATCAAGAAATAGCTGATTGCATCGCGCT
AGGTATTTCTTTCGTTAACTTTCATCCAGGAGCCGCTCTTTCTGATTCGAAAGAAAGCTGCCTAGATAGAGCGATTACTA
GTTTTTCACAAATGGCACCGCTTTTCGAAACAAATCCCCCACTTGTTGTTCTCCTTGAAACTACTGCGGGCCAAGGCTCC
TTAATAGGAAGCTCTTTCGAAGAACTGGCTTATCTGATTCAAGGAATCAAAGCTCATATACCCGTAGGAGTTTGTTTAGA
CACCTGCCATATCTTTGCCGCTGGGTATGATATCTCTTCCCCCGAAGGGTGGGAACAAGTGTTAAAACATTTTGACGAAG
TAATTGGGCTTTCTTTTTTACGAGCCATCCATTTGAATGACTCTATTTTTCCTCTTGGAAAGAATAAGGATCGCCATGCC
CCTATCGGAGAAGGTTGTATAGGTTCAGAAAGCTTTTGCTTTCTTATGCGAGATGAACGTACACGCAAGCTTCCAAAGTA
TCTAGAGACTCCAGGAGGTCCCGATTTATGGACAAAAGAGATTCGCTATCTACAGAAGGTTAGCTAA

Upstream 100 bases:

>100_bases
AATGCAATAACTCCCACTATTCTCGCTATTTATAGTTTCTTTAATCGAGAGCCTCTGGCAATCTTCTTTTATCACAAATG
ATTGGATAGAGCACCGAGCA

Downstream 100 bases:

>100_bases
AATTAGCTAACCTCTTAAAAACCCATTCTTTTTACAAGCAAGAAAAAGCCCCGCAAAAGCGGGGCAATCAAAAACCTTAC
CGTTTCAATTCACATGAACT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MFVLPPPQEPLLGAHTSAAGGLHNALYEGRDIGATTIQLFTANQRQWKRRSLTQNMIEQFQTALDETSLSYIMSHAGYLN
NPGAPNPEILEKTRICMYQEIADCIALGISFVNFHPGAALSDSKESCLDRAITSFSQMAPLFETNPPLVVLLETTAGQGS
LIGSSFEELAYLIQGIKAHIPVGVCLDTCHIFAAGYDISSPEGWEQVLKHFDEVIGLSFLRAIHLNDSIFPLGKNKDRHA
PIGEGCIGSESFCFLMRDERTRKLPKYLETPGGPDLWTKEIRYLQKVS

Sequences:

>Translated_288_residues
MFVLPPPQEPLLGAHTSAAGGLHNALYEGRDIGATTIQLFTANQRQWKRRSLTQNMIEQFQTALDETSLSYIMSHAGYLN
NPGAPNPEILEKTRICMYQEIADCIALGISFVNFHPGAALSDSKESCLDRAITSFSQMAPLFETNPPLVVLLETTAGQGS
LIGSSFEELAYLIQGIKAHIPVGVCLDTCHIFAAGYDISSPEGWEQVLKHFDEVIGLSFLRAIHLNDSIFPLGKNKDRHA
PIGEGCIGSESFCFLMRDERTRKLPKYLETPGGPDLWTKEIRYLQKVS
>Mature_288_residues
MFVLPPPQEPLLGAHTSAAGGLHNALYEGRDIGATTIQLFTANQRQWKRRSLTQNMIEQFQTALDETSLSYIMSHAGYLN
NPGAPNPEILEKTRICMYQEIADCIALGISFVNFHPGAALSDSKESCLDRAITSFSQMAPLFETNPPLVVLLETTAGQGS
LIGSSFEELAYLIQGIKAHIPVGVCLDTCHIFAAGYDISSPEGWEQVLKHFDEVIGLSFLRAIHLNDSIFPLGKNKDRHA
PIGEGCIGSESFCFLMRDERTRKLPKYLETPGGPDLWTKEIRYLQKVS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=277, Percent_Identity=42.2382671480144, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17531193, Length=266, Percent_Identity=42.8571428571429, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322735, Length=288, Percent_Identity=33.6805555555556, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CHLMU (Q9PJB8)

Other databases:

- EMBL:   AE002160
- PIR:   B81650
- RefSeq:   NP_297286.1
- ProteinModelPortal:   Q9PJB8
- SMR:   Q9PJB8
- GeneID:   1246283
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0914
- TIGR:   TC_0914
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q9PJB8
- ProtClustDB:   PRK01060
- BioCyc:   CMUR243161:TC_0914-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31814; Mature: 31814

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLPPPQEPLLGAHTSAAGGLHNALYEGRDIGATTIQLFTANQRQWKRRSLTQNMIEQF
CCCCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
QTALDETSLSYIMSHAGYLNNPGAPNPEILEKTRICMYQEIADCIALGISFVNFHPGAAL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SDSKESCLDRAITSFSQMAPLFETNPPLVVLLETTAGQGSLIGSSFEELAYLIQGIKAHI
CCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
PVGVCLDTCHIFAAGYDISSPEGWEQVLKHFDEVIGLSFLRAIHLNDSIFPLGKNKDRHA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
PIGEGCIGSESFCFLMRDERTRKLPKYLETPGGPDLWTKEIRYLQKVS
CCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFVLPPPQEPLLGAHTSAAGGLHNALYEGRDIGATTIQLFTANQRQWKRRSLTQNMIEQF
CCCCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
QTALDETSLSYIMSHAGYLNNPGAPNPEILEKTRICMYQEIADCIALGISFVNFHPGAAL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SDSKESCLDRAITSFSQMAPLFETNPPLVVLLETTAGQGSLIGSSFEELAYLIQGIKAHI
CCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
PVGVCLDTCHIFAAGYDISSPEGWEQVLKHFDEVIGLSFLRAIHLNDSIFPLGKNKDRHA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
PIGEGCIGSESFCFLMRDERTRKLPKYLETPGGPDLWTKEIRYLQKVS
CCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935