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The map label for this gene is folA

Identifier: 15835516

GI number: 15835516

Start: 1044892

End: 1045371

Strand: Reverse

Name: folA

Synonym: TC0902

Alternate gene names: 15835516

Gene position: 1045371-1044892 (Counterclockwise)

Preceding gene: 15835517

Following gene: 15835515

Centisome position: 97.43

GC content: 41.25

Gene sequence:

>480_bases
ATGATTCAAGCGACAGGAATCGTTGCTATAGATCCTCGAGGAGTTATGGGGGGGGCTGGAAAGCTCCCATGGAACTATCC
AGAGGATTTGCGTTTCTTTTCAGAGACCATTCAGGATCACCCGATCATTATGGGTAGAAAGACTTGGGAGTCTCTTCCTG
ATAGATATAAGTGTGGTCGAACCGTTATTGTTTTTTCTCGACAGCATAGCTGTGCTCAAGGTATTTGGATCTCTTCCTTA
GTAGAGTACGAGAAGTTATCTCTTAACTCTCCATTCTTAATTGGCGGAGCCGAGCTCTTTGACTGGTTTTTTCAATACAA
TCTTCTGAAATCTTGTTTTGTGACGCATATTAAAAGAGAATACCAGGGAGATACTTTTTTCCCTGTGGAGCGTTTGTCGG
GATGGAAAAGGGAGAGTGTTCTTAAAACGGAGGATTTTAATATTTATCATTATGAAAATTACGCCAATCAAAACCCGTAA

Upstream 100 bases:

>100_bases
CTGTGGCTCTTCATAATCAAGGTGTTGATTACTTGCGTGTACACCAAGTGGAAGGAAATAGACGCGTTCTAGCTGCTGCT
GCATGGTCTGGAATGCCTGT

Downstream 100 bases:

>100_bases
GGTATTTGCTCACGATTCTCTACAGGAGATTTTTCAAGAATCTTTGCCTCCTATTCAAGAACGCAGCATTGTGGTAGTCT
CTTCTAAGATTGTAAGCTTA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIQATGIVAIDPRGVMGGAGKLPWNYPEDLRFFSETIQDHPIIMGRKTWESLPDRYKCGRTVIVFSRQHSCAQGIWISSL
VEYEKLSLNSPFLIGGAELFDWFFQYNLLKSCFVTHIKREYQGDTFFPVERLSGWKRESVLKTEDFNIYHYENYANQNP

Sequences:

>Translated_159_residues
MIQATGIVAIDPRGVMGGAGKLPWNYPEDLRFFSETIQDHPIIMGRKTWESLPDRYKCGRTVIVFSRQHSCAQGIWISSL
VEYEKLSLNSPFLIGGAELFDWFFQYNLLKSCFVTHIKREYQGDTFFPVERLSGWKRESVLKTEDFNIYHYENYANQNP
>Mature_159_residues
MIQATGIVAIDPRGVMGGAGKLPWNYPEDLRFFSETIQDHPIIMGRKTWESLPDRYKCGRTVIVFSRQHSCAQGIWISSL
VEYEKLSLNSPFLIGGAELFDWFFQYNLLKSCFVTHIKREYQGDTFFPVERLSGWKRESVLKTEDFNIYHYENYANQNP

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=32.2981366459627, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17505899, Length=147, Percent_Identity=31.9727891156463, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18540; Mature: 18540

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQATGIVAIDPRGVMGGAGKLPWNYPEDLRFFSETIQDHPIIMGRKTWESLPDRYKCGR
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHCCHHHHCCC
TVIVFSRQHSCAQGIWISSLVEYEKLSLNSPFLIGGAELFDWFFQYNLLKSCFVTHIKRE
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
YQGDTFFPVERLSGWKRESVLKTEDFNIYHYENYANQNP
HCCCCCCCHHHHCCCCHHCCEECCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIQATGIVAIDPRGVMGGAGKLPWNYPEDLRFFSETIQDHPIIMGRKTWESLPDRYKCGR
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHCCHHHHCCC
TVIVFSRQHSCAQGIWISSLVEYEKLSLNSPFLIGGAELFDWFFQYNLLKSCFVTHIKRE
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
YQGDTFFPVERLSGWKRESVLKTEDFNIYHYENYANQNP
HCCCCCCCHHHHCCCCHHCCEECCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA