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Identifier: 15835515

GI number: 15835515

Start: 1044189

End: 1044920

Strand: Reverse

Name: Not Available

Synonym: TC0901

Alternate gene names: 15835515

Gene position: 1044920-1044189 (Counterclockwise)

Preceding gene: 15835516

Following gene: 15835514

Centisome position: 97.39

GC content: 40.3

Gene sequence:

>732_bases
ATGAAAATTACGCCAATCAAAACCCGTAAGGTATTTGCTCACGATTCTCTACAGGAGATTTTTCAAGAATCTTTGCCTCC
TATTCAAGAACGCAGCATTGTGGTAGTCTCTTCTAAGATTGTAAGCTTATGTGAAGGAGCTGTTGCTGATGCACAGATAA
GTAAGGCTGACTTAATCAAAAAGGAAGCGGATGCGTATTTATTCTGTGAGGGAAGCGGTATATACCTAACAAAAAAAGAG
GGAATTTTAATTCCCTCTGCAGGAATTGATGAATCCAATACAGATCAAGCCTTTGTTTTGTATCCTAAAGATCTTTTGAG
ATCGTGTAATCAAATCGGGGAATGGTTGAAAAATTATTTCCGAGTGAAAGAGTTGGGTGTAATTATCACAGATAGCCATA
CTACGCCTATGCGTCGAGGAGTAGTGGGGATAGGATTATGTTGGTATGGCTTTTCTCCATTGCATAATTATGTAGGATCT
CTGGATTGTTTTGATCGGCCACTGAAGATGACGCAGAGTAATCTTTTGGATTCTTTAGCAGCAGCAGCGGTTGTTTGTAT
GGGGGAAGGGAATGAGCAAACGCCTTTAGCGGTGATTGAACAGGCGCCTAAGGTCGTGTATCATCAACATCCTACATCTC
AAGAAGAATACTTTTCCTTACGCATAGATGAAACAGAAGATTTATATGGGCCGCTTTTGCATGCGGTTTTGTGGAATCAA
GAAAAGGAATAA

Upstream 100 bases:

>100_bases
AAAAGAGAATACCAGGGAGATACTTTTTTCCCTGTGGAGCGTTTGTCGGGATGGAAAAGGGAGAGTGTTCTTAAAACGGA
GGATTTTAATATTTATCATT

Downstream 100 bases:

>100_bases
AAGAGGTGAGCATGAATTTTTTAGATCAGCTAGATGCAATTATTCAAAACAAACATATGTTAGAACACCCTTTTTACATG
AAGTGGTCAAAAGGAGAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKITPIKTRKVFAHDSLQEIFQESLPPIQERSIVVVSSKIVSLCEGAVADAQISKADLIKKEADAYLFCEGSGIYLTKKE
GILIPSAGIDESNTDQAFVLYPKDLLRSCNQIGEWLKNYFRVKELGVIITDSHTTPMRRGVVGIGLCWYGFSPLHNYVGS
LDCFDRPLKMTQSNLLDSLAAAAVVCMGEGNEQTPLAVIEQAPKVVYHQHPTSQEEYFSLRIDETEDLYGPLLHAVLWNQ
EKE

Sequences:

>Translated_243_residues
MKITPIKTRKVFAHDSLQEIFQESLPPIQERSIVVVSSKIVSLCEGAVADAQISKADLIKKEADAYLFCEGSGIYLTKKE
GILIPSAGIDESNTDQAFVLYPKDLLRSCNQIGEWLKNYFRVKELGVIITDSHTTPMRRGVVGIGLCWYGFSPLHNYVGS
LDCFDRPLKMTQSNLLDSLAAAAVVCMGEGNEQTPLAVIEQAPKVVYHQHPTSQEEYFSLRIDETEDLYGPLLHAVLWNQ
EKE
>Mature_243_residues
MKITPIKTRKVFAHDSLQEIFQESLPPIQERSIVVVSSKIVSLCEGAVADAQISKADLIKKEADAYLFCEGSGIYLTKKE
GILIPSAGIDESNTDQAFVLYPKDLLRSCNQIGEWLKNYFRVKELGVIITDSHTTPMRRGVVGIGLCWYGFSPLHNYVGS
LDCFDRPLKMTQSNLLDSLAAAAVVCMGEGNEQTPLAVIEQAPKVVYHQHPTSQEEYFSLRIDETEDLYGPLLHAVLWNQ
EKE

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27135; Mature: 27135

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITPIKTRKVFAHDSLQEIFQESLPPIQERSIVVVSSKIVSLCEGAVADAQISKADLIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEHHHHHHHHCCCHHHHHHHHHHHHH
KEADAYLFCEGSGIYLTKKEGILIPSAGIDESNTDQAFVLYPKDLLRSCNQIGEWLKNYF
HCCCEEEEECCCCEEEECCCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
RVKELGVIITDSHTTPMRRGVVGIGLCWYGFSPLHNYVGSLDCFDRPLKMTQSNLLDSLA
HHHHCCEEEECCCCCHHHHCCEEEEHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAAVVCMGEGNEQTPLAVIEQAPKVVYHQHPTSQEEYFSLRIDETEDLYGPLLHAVLWNQ
HHHEEEECCCCCCCCHHHHHHCCHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
EKE
CCC
>Mature Secondary Structure
MKITPIKTRKVFAHDSLQEIFQESLPPIQERSIVVVSSKIVSLCEGAVADAQISKADLIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEHHHHHHHHCCCHHHHHHHHHHHHH
KEADAYLFCEGSGIYLTKKEGILIPSAGIDESNTDQAFVLYPKDLLRSCNQIGEWLKNYF
HCCCEEEEECCCCEEEECCCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
RVKELGVIITDSHTTPMRRGVVGIGLCWYGFSPLHNYVGSLDCFDRPLKMTQSNLLDSLA
HHHHCCEEEECCCCCHHHHCCEEEEHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAAVVCMGEGNEQTPLAVIEQAPKVVYHQHPTSQEEYFSLRIDETEDLYGPLLHAVLWNQ
HHHEEEECCCCCCCCHHHHHHCCHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
EKE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]