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Identifier: 15835513

GI number: 15835513

Start: 1042096

End: 1043376

Strand: Direct

Name: Not Available

Synonym: TC0899

Alternate gene names: 15835513

Gene position: 1042096-1043376 (Clockwise)

Preceding gene: 15835512

Following gene: 161579043

Centisome position: 97.12

GC content: 40.75

Gene sequence:

>1281_bases
ATGTTGCATCAGCATCAAACAGCATCTGTAGCTCTTTGTCCTGCTTTACATATTCAACAAAGTTTGGACATGCTACAGAT
GCCGGTTGCTGAACTCTCGGAATTCTTATCTCAACAAATCACTATCAATCCTTGTTTCGATCTCGATGCCCTGGACGATC
TTCCAGACACATGTTCCTTTTTCCCTATTAGTGAGCACCATCCTTTCACAGAGACCCTATTATCCCATCTACTTCACCAA
ATAGAGGTACATTTTTCTCTCCCTCAAGACCGTGCTATAGCCCAATACATCGTAGGGAATCTTTCTCCTGAAGGACTCTT
TTTGGAAGATCCCTCTCTCTCGGCTTCAAATTTAGGAGTTTCTGATGTCCTTTTTCAAAAAATATGGCTACGCATCCAAC
AATTTCATCCCCTAGGAATTGCCTCATCTTCTCTTCAATCCTATTGGCTATCTTTACTAGATCCTTCTTCGCATAAAGAA
GCTTTAATGATTATCCGACAGCACTTTAATCGATTAGCTCGTTGTGATTTTGCCGGCATTTCTAAGAAAATTCAGCTGCC
CATAGCAAAGATTCTTGTATTTCTTAGACAAGCTCTAGCTTCCATCCCCTGGTGTCCAGCAGCTGGATTTCCTAATTCAC
TTAAAACTCCTTCTCCAGCGCTTCCTGATGCCTATCTTTCTTTCTCACACGAAAAATCTTGGGAAATCTTTATGAACAAA
GATTCTCTTCCAGCTATTAGACTTAATAGTACGGTGCTTCATATTTACCACACCCTCCCCCGTGATGATAAAGACCACTT
ATCACAACAAATTCGTGCTGCAAAACAGCTGCTTCGCAATATAAAAAAACGTGAAGAAACTTTATTAGCAGTCCTTCGCG
TTTTAATTCCTTATCAGGAGGAATTCCTTCTTAAAAAACGTTCTGTTCCGAAAGCTTTTTCAGTAAAACAACTAGCTCGC
GAACTCTCTCTTCATGAAACCACTATTTGTCGGGCAATCAATAATAAGACGCTCGCAACACCTATCGGATTGATTTCTAT
GCGGTCGCTATTTCCACAAGCTGTTGGAGCTTGTCCTGATCAATCTAAAGCTACTATTTTACATTGGATACATCAGTGGA
TATCTACAGAAAAAAATCCTCTATCTGACGAGGCTATTAGCCAAAAAATTATTGCGAAAGGAATTCCTTGTGCACGCCGT
ACAGTAGCAAAATATCGGTCTCAACTAAACATCCCCCCAGCGCATCAGCGTAAACATGTGAATGCTTCTCTAGTGCCATA
A

Upstream 100 bases:

>100_bases
TCGAGCAAAAACCCGATTCCTTTGGGGGACGGAACGCACCATGGTCCCAAGCAGATTTATTAGCGAGCTGCCTAGGGCTC
TTTTAAAATTTGTTTAACCT

Downstream 100 bases:

>100_bases
TAGAGCCGTTTCGAAATCTAATCTAAATGTAAAGCAGCTTTACATACCTTCACAGCACATAAAAAAGCCCCGGGATAAGT
TTTTTATCCTGGGGCAAATA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLHQHQTASVALCPALHIQQSLDMLQMPVAELSEFLSQQITINPCFDLDALDDLPDTCSFFPISEHHPFTETLLSHLLHQ
IEVHFSLPQDRAIAQYIVGNLSPEGLFLEDPSLSASNLGVSDVLFQKIWLRIQQFHPLGIASSSLQSYWLSLLDPSSHKE
ALMIIRQHFNRLARCDFAGISKKIQLPIAKILVFLRQALASIPWCPAAGFPNSLKTPSPALPDAYLSFSHEKSWEIFMNK
DSLPAIRLNSTVLHIYHTLPRDDKDHLSQQIRAAKQLLRNIKKREETLLAVLRVLIPYQEEFLLKKRSVPKAFSVKQLAR
ELSLHETTICRAINNKTLATPIGLISMRSLFPQAVGACPDQSKATILHWIHQWISTEKNPLSDEAISQKIIAKGIPCARR
TVAKYRSQLNIPPAHQRKHVNASLVP

Sequences:

>Translated_426_residues
MLHQHQTASVALCPALHIQQSLDMLQMPVAELSEFLSQQITINPCFDLDALDDLPDTCSFFPISEHHPFTETLLSHLLHQ
IEVHFSLPQDRAIAQYIVGNLSPEGLFLEDPSLSASNLGVSDVLFQKIWLRIQQFHPLGIASSSLQSYWLSLLDPSSHKE
ALMIIRQHFNRLARCDFAGISKKIQLPIAKILVFLRQALASIPWCPAAGFPNSLKTPSPALPDAYLSFSHEKSWEIFMNK
DSLPAIRLNSTVLHIYHTLPRDDKDHLSQQIRAAKQLLRNIKKREETLLAVLRVLIPYQEEFLLKKRSVPKAFSVKQLAR
ELSLHETTICRAINNKTLATPIGLISMRSLFPQAVGACPDQSKATILHWIHQWISTEKNPLSDEAISQKIIAKGIPCARR
TVAKYRSQLNIPPAHQRKHVNASLVP
>Mature_426_residues
MLHQHQTASVALCPALHIQQSLDMLQMPVAELSEFLSQQITINPCFDLDALDDLPDTCSFFPISEHHPFTETLLSHLLHQ
IEVHFSLPQDRAIAQYIVGNLSPEGLFLEDPSLSASNLGVSDVLFQKIWLRIQQFHPLGIASSSLQSYWLSLLDPSSHKE
ALMIIRQHFNRLARCDFAGISKKIQLPIAKILVFLRQALASIPWCPAAGFPNSLKTPSPALPDAYLSFSHEKSWEIFMNK
DSLPAIRLNSTVLHIYHTLPRDDKDHLSQQIRAAKQLLRNIKKREETLLAVLRVLIPYQEEFLLKKRSVPKAFSVKQLAR
ELSLHETTICRAINNKTLATPIGLISMRSLFPQAVGACPDQSKATILHWIHQWISTEKNPLSDEAISQKIIAKGIPCARR
TVAKYRSQLNIPPAHQRKHVNASLVP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=28.3018867924528, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 48054; Mature: 48054

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHQHQTASVALCPALHIQQSLDMLQMPVAELSEFLSQQITINPCFDLDALDDLPDTCSF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCCCCCCHHCCCCCCCC
FPISEHHPFTETLLSHLLHQIEVHFSLPQDRAIAQYIVGNLSPEGLFLEDPSLSASNLGV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCH
SDVLFQKIWLRIQQFHPLGIASSSLQSYWLSLLDPSSHKEALMIIRQHFNRLARCDFAGI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCC
SKKIQLPIAKILVFLRQALASIPWCPAAGFPNSLKTPSPALPDAYLSFSHEKSWEIFMNK
CCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECC
DSLPAIRLNSTVLHIYHTLPRDDKDHLSQQIRAAKQLLRNIKKREETLLAVLRVLIPYQE
CCCCEEEECCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EFLLKKRSVPKAFSVKQLARELSLHETTICRAINNKTLATPIGLISMRSLFPQAVGACPD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHCCCCC
QSKATILHWIHQWISTEKNPLSDEAISQKIIAKGIPCARRTVAKYRSQLNIPPAHQRKHV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCCC
NASLVP
CCCCCC
>Mature Secondary Structure
MLHQHQTASVALCPALHIQQSLDMLQMPVAELSEFLSQQITINPCFDLDALDDLPDTCSF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCCCCCCHHCCCCCCCC
FPISEHHPFTETLLSHLLHQIEVHFSLPQDRAIAQYIVGNLSPEGLFLEDPSLSASNLGV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCH
SDVLFQKIWLRIQQFHPLGIASSSLQSYWLSLLDPSSHKEALMIIRQHFNRLARCDFAGI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCC
SKKIQLPIAKILVFLRQALASIPWCPAAGFPNSLKTPSPALPDAYLSFSHEKSWEIFMNK
CCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECC
DSLPAIRLNSTVLHIYHTLPRDDKDHLSQQIRAAKQLLRNIKKREETLLAVLRVLIPYQE
CCCCEEEECCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EFLLKKRSVPKAFSVKQLARELSLHETTICRAINNKTLATPIGLISMRSLFPQAVGACPD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHCCCCC
QSKATILHWIHQWISTEKNPLSDEAISQKIIAKGIPCARRTVAKYRSQLNIPPAHQRKHV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCCC
NASLVP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]